Involvement of gicerin, a cell adhesion molecule, in the portal metastasis of rat colorectal adenocarcinoma cells

Abstract

Abstract. Gicerin, an Ig-superfamily cell adhesion molecule, has homophilic adhesion activity, thus leading to the formation of gicerin aggregates. Gicerin is highly expressed in various embryonic tissues, and it contributes to development through its adhesive activities. In contrast, the expression of the protein is limited to the muscular tissues and endothelial cells in the mature animals. In the liver, gicerin is constitutively expressed in sinusoidal endothelial cells. Interestingly, an overexpression of gicerin is found in a variety of tumors and may play a role in tumorigenesis. Previously, up-regulated expression of the gicerin protein was found in some sporadic cases of chicken colorectal adenocarcinomas and their hepatic metastasized lesions. In the present study, gicerin cDNA was introduced into endogenous gicerin negative ACL-15 cells, a rat colon adenocarcinoma cell line. The cells were subsequently evaluated for changes in their metastatic potentials in order to elucidate the possible role of gicerin in the hepatic metastasis of colorectal adenocarcinomas. The stable overexpression of gicerin in the cells enhanced the self-aggregation and migratory activities on the protein compared with the mock-transfectants. In addition, the gicerintransfectants had enhanced metastatic potential to the liver compared with mock-transfected cells after implantation into the ileocolic vein of the cognate rats. These results suggest that gicerin might promote the interaction of tumor cells with a hepatic endothelium, thus leading to the hepatic metastasis of colon adenocarcinomas.

Review and prospect of CD146 research

“…在肿瘤发生发展过程中, CD146既影响肿瘤细胞 [66] ; 促侵袭迁移 [202] ; 促EMT [80] ; 维持干细胞干性 [238] 可作为总体生存率低, 复发期短的指标 [39,219] ; 可作为肿瘤成像靶标 [219] 结直肠癌 促转移 [233] ; 抑制干细胞干性 [42] ; 抑制肿瘤的发生和耐药 [42] 与肿瘤组织学分级、杜克分期和肝转移线性相关, 可作为术后肝转移的标志物 [213] 胆囊腺癌 促侵袭转移 [200] 可作为总体生存率较低的预后指标 [200] 食管鳞癌 促淋巴结转移 [204] 与肿瘤临床分期正相关, 可作为不良预后的标志物 [204] 胃癌 促EMT [207] 与淋巴结受累和预后不良正相关 [207] 胰腺导管腺癌 促转移 [274] -腺样囊性癌 促增殖 [220] 与神经侵袭性相关 [220] 生殖系统 乳腺癌 促增殖、抗凋亡 [240,275] ; 促EMT(有争 议) [138,208,215,240] ; 促侵袭迁移(有争 议) [34,122,138,208,276~278] ; 维持三阴性表 型 [34,208] ; 促耐药 [118,240] 与乳腺癌的恶性程度和转移性正相关, 可作为肿瘤分期 高、预后差、生存期短的指标 [34,208,209,215] ; 可作为三阴 性乳腺癌的诊断指标 [34,215] 卵巢癌 促增殖(有争议) [36,226,279] ; 促EMT [67] ; 促进侵袭迁移(有争议) [36,205,280] 与卵巢癌恶性程度正相关, 是早期复发和预后较差的 指标 [67,205,...…”