Gibberellin transport

Dayan, Jonathan

doi:10.1002/9781119210436.ch4

Cited by 10 publications

(9 citation statements)

References 96 publications

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“…The 2986 DEGs identified in this study represent a large number of enriched GO terms which overlapped in the three tissues in various degrees and response states, reflecting not only the breadth of GA signaling cascades [55] but also the dynamic coordination of biological functions within and between tissues [1, 60]. For example, one of the larger GO biological terms was related to meristem regulation and pattern-specification processes and largely enriched among the suppressed DEGs in the shoot and leaf.…”

Section: Discussionmentioning

confidence: 99%

“…Gibberellins arguably has the widest role to play in the whole life cycle of a plant. Plants tightly regulate the availability of GA by coordinating the activation and deactivation of multiple biosynthesis genes of GA in different tissues at various developmental stages [1]. The flow rate and availability level of bioactive GA in various tissues are regulated by a de-repressible system in which GA interacts with its receptor GA INSENSITIVE DWARF1 (GID1) and negative regulator, DELLA, to form a trimeric GA:GID1:DELLA complex [2].…”

Section: Introductionmentioning

confidence: 99%

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RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines

Arro¹,

Yang²,

Song

et al. 2019

BMC Plant Biol

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Background Gibberellins (GAs) and their regulator DELLA are involved in many aspects of plant growth and development and most of our current knowledge in the DELLA-facilitated GA signaling was obtained from the studies of annual species. To understand GA-DELLA signaling in perennial species, we created ten GA-insensitive transgenic grapevines carrying a DELLA mutant allele ( Vvgai1 ) in the background of Vitis vinifera ‘Thompson Seedless’ and conducted comprehensive analysis of their RNA expression profiles in the shoot, leaf and root tissues. Results The transgenic lines showed varying degrees of dwarf stature and other typical DELLA mutant phenotypes tightly correlated with the levels of Vvgai1 expression. A large number of differentially expressed genes (DEGs) were identified in the shoot, leaf and root tissues of the transgenic lines and these DEGs were involved in diverse biological processes; many of the DEGs showed strong tissue specificity and about 30% them carried a DELLA motif. We further discovered unexpected expression patterns of several key flowering induction genes VvCO, VvCOL1 and VvTFL1 . Conclusions Our results not only confirmed many previous DELLA study findings in annual species, but also revealed new DELLA targets and responses in grapevine, including the roles of homeodomain transcription factors as potential co-regulators with DELLA in controlling the development of grapevine which uniquely possess both vegetative and reproductive meristems at the same time. The contrasting responses of some key flowering induction pathway genes provides new insights into the divergence of GA-DELLA regulations between annual and perennial species in GA-DELLA signaling. Electronic supplementary material The online version of this article (10.1186/s12870-019-1675-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines

Arro¹,

Yang²,

Song

et al. 2019

BMC Plant Biol

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“…Email: pbngoc@ibt.ac.vn https://doi.org/10.25073/2588-1140/vnunst.4586 và có ảnh hưởng đến các quá trình phát triển khác nhau ở th c v t bao gồm s nảy mầm của h t sinh trưởng của thân, ra hoa và t o quả. GA ũng đượ biết đến với v i trò k h th h phân hi tế b o ở tầng phát hi n v tăng trưởng nhanh chóng ủ á sợi xylem kéo d i. Cho đến n y đã xá định được hơn 136 chất thuộc nhóm này, tuy nhiên chỉ một vài chất là có ho t tính sinh h c, ví dụ như GA1 v GA4 [1].…”

Section: Mở đầU unclassified

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Nhung¹,

Thảo²,

Đoài³

et al. 2017

NST

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Tóm tắt: Nhiều ng trình nghiên cứu trên thế giới về chuyển gen mã hóa cho GA20-oxidase vào th c v t đã t o được các dòng chuyển genGA20ox có tác dụng khả năng kéo d i thân v gi tăng sinh kh i. Tuy nhiên, một s tác giả nh n thấy s biểu hi n quá mức của GA20ox dưới s điều khiển củ promoter ơ định mang l i những tính tr ng bất lợi ho ây như giảm k h thước lá và s phát triển của rễ. Trong nghiên cứu này, vector chuyển gen nhị thể pBI121 chứa gen GA20ox mã hóa cho GA20-oxid se dưới s điều khiển củ promoter CAD4 đặc hi u ở xylem (phân l p từ ây Dương l i (Populus trichocarpa) đã được thiết kế. Vector thiết kế này được chuyển gen vào gi ng Thu c lá (Nicotiana tabacum) K326 thông qua vi khuẩn Agrobacterium tumefaciens và thu được kết quả gen chuyển được biểu hi n đặc hi u ở vùng xylem qua phản ứng RT-PCR. Các dòng thu c lá chuyển gen đều có khả năng sinh trưởng t t hơn so với mẫu đ i chứng, chiều o ây tăng từ 20-60%, t độ sinh trưởng trung bình gấp đến 1,66 lần. Gen chuyển GA20ox dưới s điều khiển củ promoter CAD4 tăng khả năng bi t hó xylem độ rộng vùng xylem của các dòng chuyển gen tăng l n từ 17-59% so với ây đ i chứng. Vector chuyển gen mã hóa cho GA20-oxid se dưới s điều khiển củ promoter CAD4 được thiết kế và chuyển gen vào cây mô hình có thể được sử dụng trong t o gi ng cây trồng lâm nghi p có khả năng sinh trưởng nhanh và cho sinh kh i gỗ lớn.

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“…The last two decades have witnessed plant growth and biomass improvements by the transgenic expression of the GA20ox gene in different plant species [4][5][6][7][8][9][10][11][12][13]. GA20ox is a key enzyme involved in the gibberellin biosynthesis (GAs) pathway and it directly controls the content of bioactive Ga in plants [14][15][16][17][18]. Previous studies showed that overexpression of GA20-oxidase (GA20ox) conferred desirable phenotypes, including accelerated stem growth, increased biomass production, enhanced bre cell elongation, early owering, and decreased seed dormancy [4,12,19,20].…”

Section: Introductionmentioning

confidence: 99%

Demonstration of improved plant growth and biomass production by At1g73160 promoter-controlled root-preferential expression of the AtGA20ox gene

Bui

Tien

et al. 2020

Preprint

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Background: Overexpression of the GA20 oxidase gene has been a recent trend for improving plant growth and biomass. Constitutive expression of GA20ox has successfully improved plant growth and biomass in several plant species. However, the constitutive expression of this gene causes side-effects, such as reduced leaf size and stem diameters. To avoid these effects, different tissue-specific promoters were identified and employed for GA20ox overexpression. In this study, we demonstrated the potential of At1g, a root-preferential promoter, for GA20ox expression to enhance plant biomass in tobacco and Melia azedarach.Results: We examined the utility of the At1g promoter to drive the expression of a GUS (β-1,4-glucuronidase) reporter and the GA20ox gene in tobacco and Melia azedarach. Histochemical GUS assays in tobacco showed that At1g was a root-preferential promoter whose expression was particularly strong in root tips. The ectopic expression of the AtGA20ox gene under the control of the At1g promoter showed improved plant growth and biomass of both tobacco and M. azedarach transgenic plants compared to wild-type (WT) control plants. Stem length as well as stem and root fresh weights increased by up to 1.5-3 folds in transgenic tobacco and 2-fold in transgenic M. azedarach. Both tobacco and M. azedarach transgenic plants showed increases in the root xylem width and xylem over phloem ratio by 50%–100% compared to WT plants. Importantly, no significant differences in the leaf shape or size were observed between the At1g::AtGA20ox transgenic and WT plants. Moreover, transgenic M. azedarach showed a 135% increase in stem diameter although no change was found in transgenic tobacco.Conclusions: These results demonstrate the great utility of the At1g promoter for driving the AtGA20ox gene to induce growth and biomass improvements in woody plants and potentially some other plant species.

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Gibberellin transport

Cited by 10 publications

References 96 publications

RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines

RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines

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Demonstration of improved plant growth and biomass production by At1g73160 promoter-controlled root-preferential expression of the AtGA20ox gene

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