Gibberellin-regulated expression of a myb gene in barley aleurone cells: evidence for Myb transactivation of a high-pI alpha-amylase gene promoter.

Gubler, Frank; Kalla, Roger; Roberts, James K.; Jacobsen, John V.

doi:10.1105/tpc.7.11.1879

Cited by 354 publications

(343 citation statements)

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“…Two known targets, DDF1 and At1g26960, bound by REF6-6HA 47 Second, the forepart of the NFYBE is similar to a GAresponsive cis-element (GARE), 5 0 -TAACAAA/G-3 0 , which has been identified from an a-amylase gene in barley aleurone layers 61 . This element is bound by GAMYB, a GA-inducible transcriptional factor, which activates the a-amylase gene expression in aleurone cells 62 . In Arabidopsis, the GARE is significantly enriched in the promoters of GA-responsive genes 63 .…”

Section: Discussionmentioning

confidence: 99%

Nuclear factor Y-mediated H3K27me3 demethylation of the SOC1 locus orchestrates flowering responses of Arabidopsis

et al. 2014

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Nuclear factor Y (NF-Y) is a conserved heterotrimeric transcription factor complex that binds to the CCAAT motifs within the promoter region of many genes. In plants, a large number of genes code for variants of each NF-YA, B or C subunit that can assemble in a combinatorial fashion. Here, we report the discovery of an Arabidopsis NF-Y complex that exerts epigenetic control over flowering time by integrating environmental and developmental signals. We show that NF-Y interacts with CONSTANS in the photoperiod pathway and DELLAs in the gibberellin pathway, to directly regulate the transcription of SOC1, a major floral pathway integrator. This NF-Y complex binds to a unique cis-element within the SOC1 promoter to modulate trimethylated H3K27 levels, partly through a H3K27 demethylase REF6. Our findings establish NF-Y complexes as critical mediators of epigenetic marks that regulate the response to environmental or intrinsic signals in plants.

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Section: Discussionmentioning

confidence: 99%

Nuclear factor Y-mediated H3K27me3 demethylation of the SOC1 locus orchestrates flowering responses of Arabidopsis

et al. 2014

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“…The experiment using the maize Myb suggests that competition between Mybs binding to the GARE may be an important aspect of the GA regulation of α-amylase expression. This is especially relevant when considering that, in contrast to GAMyb, two of the other proteins recognized by the Myb antibody in the experiments of Gubler et al (1995) were downregulated by GA.…”

Section: Transcriptional Regulationmentioning

confidence: 99%

“…The GAMyb protein binds specifically to the GARE sequence (Gubler et al, 1995). Aleurone cells transformed with GAMyb also show GA-like levels of expression of a high-pI α-amylase in the absence of GA. Interestingly, a parallel experiment using a maize Myb (C1) had the opposite effect, partially inhibiting α-amylase expression in the presence of GA.…”

Section: Transcriptional Regulationmentioning

confidence: 99%

“…Gubler et al (1995) noticed that the GARE of a high-pI α-amylase gene ( pHVa19) is similar to the consensus sequence YAACKGHH (Y l C\T: K l G\T; H l A\C\T) of the binding site of the Myb group of transcription factors. Mybs are rapidly becoming recognized as a ubiquitous class of transcriptional regulators of an array of diverse plant processes (Martin & Paz-Ares, 1997).…”

Section: Transcriptional Regulationmentioning

confidence: 99%

“…Mybs are rapidly becoming recognized as a ubiquitous class of transcriptional regulators of an array of diverse plant processes (Martin & Paz-Ares, 1997). Gubler et al (1995) therefore probed protein blots from barley aleurone with antibodies against maize Myb and found three cross-reacting proteins. One of these proteins was up-regulated in GA-treated tissue whereas the others were down-regulated.…”

Section: Transcriptional Regulationmentioning

confidence: 99%

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Gibberellins: regulating genes and germination

Ritchie

Gilroy

1998

New Phytologist

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The range of processes regulated by gibberellins (GAs) covers all aspects of the life history of the plant from seed germination to vegetative growth and flowering. In seeds there has been an intensive search, using the techniques of both biochemistry and cell biology, for the regulatory molecules linking GA perception to gene regulation and the events of germination. Although a GA receptor has yet to be identified, the site of perception has been localized to the plasma membrane. Calmodulin, Ca2+ and cGMP have also been identified as elements of the GA signal transduction pathway. These regulators parallel many of the signalling elements identified in the transduction of other signals such as phytochrome and ABA. Studies of GA‐regulated gene expression, principally of the α‐amylases of cereal aleurone, have identified core GA‐responsive promoter elements, such as the gibberellin response element (GARE), box‐1 and pyrimidine boxes, as well as elements that may lend specificity to GA‐regulated expression, such as the Opaque‐2‐similar element (O2S), and TRE and CRE motifs. One of the most striking features of all of these studies of the molecular basis of GA action is the interaction of GA‐dependent regulatory elements with those of other factors such as ABA. GA‐response elements also appear to be conserved between disparate GA‐response systems. For example, Myb transcription factors appear to regulate a multitude of GA‐induced genes in cereal aleurone as well as to alter GA responses when expressed in Arabidopsis. Thus the study of GA signal transduction and response systems is highlighting the conservation of regulatory elements used by plants. These common factors, used by distinct signal transduction systems, provide a molecular basis for the integration of the GA signal with other growth regulators that is the hallmark of plant growth and development.

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