Gibberellin is required for the formation of tension wood and stem gravitropism in Acacia mangium seedlings

Nugroho, Widyanto Dwi; Yamagishi, Yusuke; Nakaba, Satoshi; Fukuhara, Shiori; Begum, Shahanara; Marsoem, Sri Nugroho; Ko, Jae‐Heung; Jin, Hyun Seung; Funada, Ryo

doi:10.1093/aob/mcs148

Cited by 42 publications

(34 citation statements)

References 44 publications

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“…The formation of tension wood on the side upper of the stem occurs to support the gravity of woody angiosperms 26 . When self-weight exceeds the tension, the branches weep.…”

Section: Discussionmentioning

confidence: 99%

“…It has also been suggested that GA function in gravitropic responses, although GA1 asymmetry does not trigger gravitropic responses 25 . Nugroho et al 26 suggested that GA has a strong influence on the initial stages during the formation of tension wood and stem gravitropism in Acacia mangium. In peach, the pl phenotype may be associated with GA signaling and upright branch internodes are shorter than those of weeping branches in peach, implying that the upright type may have a lower GA content 27 .…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome profiles reveal that gibberellin-related genes regulate weeping traits in crape myrtle

Zheng

Zhuo

et al. 2020

Hortic Res

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Plant architecture includes vital traits that influence and benefit crops, and economically important trees. Different plant architectures provide natural beauty. Weeping ornamental plants are aesthetically appealing to people. The regulatory mechanism controlling the weeping trait is poorly understood in crape myrtle. To investigate the weeping trait mechanism, transcriptional profiling of different organs in weeping and upright crape myrtle was performed based on phenotype. Phenotypic and histological analyses demonstrated that endodermal cells were absent, and that new shoot phenotypes could be rescued by the GA3 treatment of weeping plants. The transcriptional analysis and coexpression network analysis (WGCNA) of differentially expressed genes indicated that GA synthesis and signal transduction pathways play a role in weeping traits. When the expression level of a negative element of GA signaling, LfiGRAS1, was reduced by virus-induced gene silencing (VIGS), new branches grew in infected plants in a negatively geotropic manner. An integrated analysis implied that GA had a strong influence on weeping crape myrtle by interacting with other factors. This study helps to elucidate the mechanism governing the weeping trait and can improve the efficiency of breeding in Lagerstroemia.

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“…The formation of tension wood on the side upper of the stem occurs to support the gravity of woody angiosperms 26 . When self-weight exceeds the tension, the branches weep.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptome profiles reveal that gibberellin-related genes regulate weeping traits in crape myrtle

Zheng

Zhuo

et al. 2020

Hortic Res

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“…Auxin has been suggested as a key factor for not only cambial meristem maintenance, but also xylogenesis (Aloni et al ., ; Mattsson et al ., ; Moyle et al ., ; Ruzicka et al ., ; Sundberg and Little, ; Uggla et al ., ). GAs are also known to be involved in wood formation (Digby and Wareing, ; Eriksson et al ., ; Israelsson et al ., , ; Little and Pharis, ; Nugroho et al ., ; Wareing, ). Elevated levels of GAs by the overexpression of either AtGA20ox1 or AtGA20ox2 induce an increase in the number of lignified vessels, xylem fibres and increased xylem fibre length in tobacco and poplar (Biemelt et al ., ; Israelsson et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Developing xylem‐preferential expression of PdGA20ox1, a gibberellin 20‐oxidase 1 from Pinus densiflora, improves woody biomass production in a hybrid poplar

Jeon

Cho

Park

et al. 2015

Plant Biotechnology Journal

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“…For example, transgenic approaches showed that the endogenous ethylene produced in leaning trees acts as a key regulator of the asymmetrical cambial growth in Populus TW [ 9 ]. Recent work also reported that the formation of TW and stem gravitropism in Acacia mangium seedlings requires gibberellins [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Transcript profiling of Populus tomentosa genes in normal, tension, and opposite wood by RNA-seq

Chen

Zhang

2015

BMC Genomics

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BackgroundWood formation affects the chemical and physical properties of wood, and thus affects its utility as a building material or a feedstock for biofuels, pulp and paper. To obtain genome-wide insights on the transcriptome changes and regulatory networks in wood formation, we used high-throughput RNA sequencing to characterize cDNA libraries of mature xylem from tension wood (TW), opposite wood (OW), and normal wood (NW), in the industrial tree species Populus tomentosa.ResultsOur sequencing generated 140,978,316 (TW), 128,972,228 (OW), and 117,672,362 (NW) reads, corresponding to 10,127 (TW), 10,129 (OW), and 10,129 (NW) unique genes. Of these, 361 genes were differentially transcribed between TW and OW (log2FC ≥ 1 or ≤ -1, FDR < 0.05), 2,658 differed between OW and NW, and 2,417 differed between TW and NW. This indicates that NW differs significantly from the wood in branches; GO term analysis also indicated that OW experienced more transcriptome remodeling. The differentially expressed genes included 97 encoding transcription factors (TFs), 40 involved in hormone signal transduction, 33 in lignin biosynthesis, 21 in flavonoid biosynthesis, and 43 in cell wall metabolism, including cellulose synthase, sucrose synthase, and COBRA. More than half of the differentially expressed TF showed more than 4-fold lower transcript levels in NW compared with TW or OW, indicating that TF abundances differed dramatically in different wood types and may have important roles in the formation of reaction wood. In addition, transcripts of most of the genes involved in lignin biosynthesis were more abundant in OW compared with TW, consistent with the higher lignin content of OW. We constructed two transcriptomic networks for the regulation of lignin and cellulose biosynthesis, including TFs, based on the co-expression patterns of different genes. Lastly, we used reverse transcription quantitative PCR to validate the differentially expressed genes identified.ConclusionsHere, we identified the global patterns and differences in gene expression among TW, OW, and NW, and constructed two transcriptomic regulatory networks involved in TW formation in P. tomentosa. We also identified candidate genes for molecular breeding of wood quality, and provided a starting point to decipher the molecular mechanisms of wood formation in Populus.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1390-y) contains supplementary material, which is available to authorized users.

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Gibberellin is required for the formation of tension wood and stem gravitropism in Acacia mangium seedlings

Abstract: The results suggest that gibberellin plays an important role at the initial stages of formation of tension wood and in stem gravitropism in A. mangium seedlings in response to a gravitational stimulus.

Cited by 42 publications

References 44 publications

Transcriptome profiles reveal that gibberellin-related genes regulate weeping traits in crape myrtle

Transcriptome profiles reveal that gibberellin-related genes regulate weeping traits in crape myrtle

Developing xylem‐preferential expression of PdGA20ox1, a gibberellin 20‐oxidase 1 from Pinus densiflora, improves woody biomass production in a hybrid poplar

Transcript profiling of Populus tomentosa genes in normal, tension, and opposite wood by RNA-seq

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