Developing xylem‐preferential expression of <i>PdGA20ox1</i>, a gibberellin 20‐oxidase 1 from <i>Pinus densiflora</i>, improves woody biomass production in a hybrid poplar

Jeon, Hyung‐Woo; Cho, Jin Seong; Park, Eung Jun; Han, Kyung Hwan; Choi, Young‐Im; Ko, Jae‐Heung

doi:10.1111/pbi.12484

Cited by 73 publications

(84 citation statements)

References 58 publications

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“…pGA2:GA20-transformed plants showed increased leaf mass fraction, and decreased stem mass and root mass fractions. This was consistent with previous results showing that although GAs are positive regulator of root growth, they significantly repress root development (Eriksson et al 2000;Busov et al 2006;Gou et al 2010Gou et al , 2011Jeon et al 2015). The increase in leaf mass fraction in our pGA2:GA20-intragenic plants was comparable to the slight increase shown by Eriksson et al (2000).…”

Section: Discussionsupporting

confidence: 93%

“…However, under current stringent laws and regulations pertaining to genetically modified crops, studies are either restricted to greenhouse testing alone (e.g., Eriksson et al 2000;Han et al 2010;Zawaski et al 2012;Jeon et al 2015), or confined to small-scale field trials for a minimum duration of time to minimize or avoid any flowering (e.g., 3 years in Jing et al 2004; only 1 year in Kim et al 2011). Policy changes are likely to be needed to provide a more conducive environment for field trials of transgenic trees if growth rate and plant architecture-modifying transgenes, especially cisgenes and intragenes whose effects are comparable to that from conventional breeding, are to be used in breeding programs (Strauss et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…GA20ox overexpression has been used to successfully modify plant stature in Arabidopsis thaliana (Arabidopsis) ( AtGA20o x1 ; Pd GA2 0ox 1), Sola nu m tube ro sum (StGA20ox1), tobacco (NtGA20ox1), hybrid aspen (PtGA20ox1; PdGA20ox1), Carrizo citrange (CcGA20ox1) ( reviewed by Bhattacharya et al 2009;Jeon et al 2015), tomato (CcGA20ox1) (García-Hurtado et al 2012), rice (OsGA20ox3; Qin et al 2013), and Tricyrtis sp. 'Shinonome' (TfGA20ox2; Otani et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Because GAs have prominent roles in the control of many aspects of vegetative development in plants, they are logical candidates as tools for achieving these modifications. Eriksson et al (2000), Han et al (2010) and Jeon et al (2015) reported modified properties and improved biomass of poplars by transgenic or cisgenic modification of GA levels. Eriksson et al (2000) overexpressed a modified GA20ox gene (lacking an upstream ATG codon) from Arabidopsis.…”

Section: Introductionmentioning

confidence: 99%

“…1), faster growth in height and diameter, and greater shoot vs. root biomass. Similarly, Jeon et al (2015) overexpressed a Pinus densiflora GA20ox (PdGA20ox1) in poplars using a constitutive 35S promoter and a xylem-specific promoter respectively. In both cases, they observed an up to three-fold increase in stem biomass, but noticeable reduction in leaf area (20 % to 40 %) and root biomass (up to 80 %) in transgenic plants.…”

Section: Introductionmentioning

confidence: 99%

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Recombinant DNA modification of gibberellin metabolism alters growth rate and biomass allocation in Populus

Viswanath

et al. 2015

Tree Genetics & Genomes

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Overexpression of genes that modify gibberellin (GA) metabolism and signaling have been previously shown to produce trees with improved biomass production but highly disturbed development. To examine if more subtle types of genetic modification of GA could improve growth rate and modify tree architecture, we transformed a model poplar genotype (Populus tremula × P. alba) with eight genes, including two cisgenes (intact copies of native genes), four intragenes (modified copies of native genes), and two transgenes (from sexually incompatible species), and studied their effects under greenhouse and field conditions. In the greenhouse, four out of the eight tested genes produced a significant and often striking improvement of stem volume, and two constructs significantly modified the proportion of root or shoot biomass. Characterization of GA concentrations in the cisgenic population that had an additional copy of a poplar GA20-oxidase gene showed elevated concentrations of 13-hydroxylated GAs compared to wild-type poplars. In the field, we observed growth improvement for three of the six tested constructs, but it was significantly greater for only one of the constructs, a pRGL:GA20-oxidase intragene. The greenhouse and field responses were highly variable, possibly to due to cross-talk among the GA pathway and other stress response pathways, or due to interactions between the cisgenes and intragenes with highly similar endogenes. Our results indicate that extensive field trials, similar to those required for conventional breeding, will be critical to evaluating the value and pleiotropic effects of GA-modifying genes.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Recombinant DNA modification of gibberellin metabolism alters growth rate and biomass allocation in Populus

Viswanath

et al. 2015

Tree Genetics & Genomes

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EliteTree™: an advanced biomass tree crop technology that features greater wood density and accelerated stem growth

Kim

Cho

et al. 2017

Biofuels Bioprod Bioref

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Due to wood's potential for large-scale commercial production of biofuels, a rapid increase in the use of wood as a source of energy is expected as policies promoting greater use of renewable energy are adopted globally. However, the economics of purpose-grown tree feedstocks for energy show that these production systems are not fi nancially viable without improvement in the base growth rate. Conventional breeding programs have produced willow and poplar clones that show potential for rapid growth, but current top-performing clones do not grow fast enough for profi table biofuel production. Genetic manipulation of secondary wall biosynthesis is the most direct path to resolving this growth barrier. To that end, we developed an innovative biomass tree crop technology, EliteTree TM , that results in greater wood density and accelerated growth of stems. This technology is built on overexpression of Gibberellin 20-oxidase to increase plant stem growth in both height and diameter and increase lignocellulosic biomass accumulation through overexpression of the transcription factor MYB46, which is a master regulator for secondary wall biosynthesis. EliteTree TM technology uses 2A-mediated bicistronic gene expression, with our proprietary utility promoter DX15, such that the genetic manipulation is limited to wood tissue, yielding transgenic poplars with xylem-specifi c coexpression of MYB46 and PdGA20ox1. The development of faster growing elite tree genotypes with increased wood density and growth rates will pave the way for truly sustainable and economically 522

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Genetic dissection of monosaccharides contents in rice whole grain using genome‐wide association study

2023

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The simplest form of carbohydrates are monosaccharides which are the building blocks for the synthesis of polymers or complex carbohydrates. Monosaccharide contents of 197 rice accessions were quantified by HPAEC‐PAD in rice (Oryza sativa L.) whole grain (RWG). A genome‐wide association study (GWAS) was carried out using 33,812 single nucleotide polymorphisms (SNPs) to identify corresponding genomic regions influencing neutral monosaccharides contents. In total, 49 GWAS signals contained in 17 genomic regions (quantitative trait loci [QTLs]) on seven chromosomes of rice were determined to be associated with monosaccharides contents of whole grain. The QTLs were found for fucose (1), mannose (1), xylose (2), arabinose (2), galactose (4), and rhamnose (7) contents, all of which are novel. Based on co‐location of annotated rice genes in the vicinity of GWAS signals, the constituents of the whole grain were associated with the following candidate genes: arabinose content with α‐N‐arabinofuranosidase, pectinesterase inhibitor, and glucosamine‐fructose‐6‐phosphate aminotransferase 1; xylose content with ZOS1‐10 (a C2H2 zinc finger transcription factor [TF]); mannose content with aldose 1‐epimerase‐like protein and a MYB family TF; galactose content with a GT8 family member (galacturonosyltransferase‐like 3), a GRAS family TF, and a GH16 family member (xyloglucan endotransglucosylase/hydrolase xyloglucan 23); fucose content with gibberellin 20 oxidase and a lysine‐rich arabinogalactan protein 19, and finally rhamnose content with myo‐inositol‐1‐phosphate synthase, UDP‐arabinopyranose mutase, and COBRA‐like protein precursor. The results of this study should improve our understanding of the genetic basis of the factors that might be involved in the biosynthesis, regulation, and turnover of monosaccharides in RWG, aiming to enhance the nutritional value of rice grain and impact the related industries.

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Developing xylem‐preferential expression of PdGA20ox1, a gibberellin 20‐oxidase 1 from Pinus densiflora, improves woody biomass production in a hybrid poplar

Abstract: These authors contributed equally and are considered co-first authors.

Cited by 73 publications

References 58 publications

Recombinant DNA modification of gibberellin metabolism alters growth rate and biomass allocation in Populus

Recombinant DNA modification of gibberellin metabolism alters growth rate and biomass allocation in Populus

EliteTree™: an advanced biomass tree crop technology that features greater wood density and accelerated stem growth

Genetic dissection of monosaccharides contents in rice whole grain using genome‐wide association study

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