Giant meiotic spindles in males from <i>Drosophila</i> species with giant sperm tails

Lattao, Ramona; Bonaccorsi, Silvia; Gatti, Maurizio

doi:10.1242/jcs.101469

Cited by 17 publications

(8 citation statements)

References 36 publications

(46 reference statements)

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“…One gene has a spindle associated domain, and another a proteasome complex domain. These domains have been associated with sperm differentiation function 45 46 and although it is premature to speculate, the chromosomal proximity between genes, the presence of potential sperm differentiation genes among them and the large change in allelic expression ratio between hybrids highlights these genes as interesting targets for future gene function analysis in these species.…”

Section: Discussionmentioning

confidence: 99%

Hybrid male sterility and genome-wide misexpression of male reproductive proteases

Gomes

Civetta

2015

Sci Rep

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Hybrid male sterility is a common barrier to gene flow between species. Previous studies have posited a link between misregulation of spermatogenesis genes in interspecies hybrids and sterility. However, in the absence of fully fertile control hybrids, it is impossible to differentiate between misregulation associated with sterility vs. fast male gene regulatory evolution. Here, we differentiate between these two possibilities using a D. pseudoobscura species pair that experiences unidirectional hybrid sterility. We identify genes uniquely misexpressed in sterile hybrid male reproductive tracts via RNA-seq. The sterile male hybrids had more misregulated and more over or under expressed genes relative to parental species than the fertile male hybrids. Proteases were the only gene ontology class overrepresented among uniquely misexpressed genes, with four located within a previously identified hybrid male sterility locus. This result highlights the potential role of a previously unexplored class of genes in interspecific hybrid male sterility and speciation.

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Section: Discussionmentioning

confidence: 99%

Hybrid male sterility and genome-wide misexpression of male reproductive proteases

Gomes

Civetta

2015

Sci Rep

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“…The Drosophila male meiosis I spindle is some 23.3 ± 0.2 µm long compared to a NB mitotic spindle of 11.7 ± 3.3 µm. It has been proposed that these huge spindles of the primary spermatocytes provide effective stores of tubulin needed for subsequent sperm tail formation (Lattao et al 2012). Centrosomes and centrioles are essential for the assembly of these spindles (Bonaccorsi et al 1998; Rebollo et al 2004; Giansanti et al 2008) and axoneme formation during spermiogenesis (Bettencourt-Dias et al 2005), respectively.…”

Section: When the Centriole Becomes The Basal Body: Ciliogenesismentioning

confidence: 99%

The Centrioles,Centrosomes, Basal Bodies, and Cilia of Drosophila melanogaster

Lattao¹,

Kovács²,

Glover

2017

Genetics

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Centrioles play a key role in the development of the fly. They are needed for the correct formation of centrosomes, the organelles at the poles of the spindle that can persist as microtubule organizing centers (MTOCs) into interphase. The ability to nucleate cytoplasmic microtubules (MTs) is a property of the surrounding pericentriolar material (PCM). The centriole has a dual life, existing not only as the core of the centrosome but also as the basal body, the structure that templates the formation of cilia and flagellae. Thus the structure and functions of the centriole, the centrosome, and the basal body have an impact upon many aspects of development and physiology that can readily be modeled in Drosophila. Centrosomes are essential to give organization to the rapidly increasing numbers of nuclei in the syncytial embryo and for the spatially precise execution of cell division in numerous tissues, particularly during male meiosis. Although mitotic cell cycles can take place in the absence of centrosomes, this is an error-prone process that opens up the fly to developmental defects and the potential of tumor formation. Here, we review the structure and functions of the centriole, the centrosome, and the basal body in different tissues and cultured cells of Drosophila melanogaster, highlighting their contributions to different aspects of development and cell division.

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“…While multiple components could become limiting, we focused our attention on tubulin, the subunit of microtubules and the major structural component of the spindle whose levels have been implicated in regulating spindle size (23). Because cellular tubulin concentration and the number and length of microtubules in the egg extract spindle have been characterized (24, 25), it was possible to determine what fraction of soluble tubulin within a given volume remained in the cytoplasm after spindle assembly.…”

mentioning

confidence: 99%

Cytoplasmic Volume Modulates Spindle Size During Embryogenesis

Good

Vahey

Skandarajah

et al. 2013

Science

236

278

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Rapid and reductive cell divisions during embryogenesis require that intracellular structures adapt to a wide range of cell sizes. The mitotic spindle presents a central example of this flexibility, scaling with the dimensions of the cell to mediate accurate chromosome segregation. To determine whether spindle size regulation is achieved through a developmental program or is intrinsically specified by cell size or shape, we developed a system to encapsulate cytoplasm from Xenopus eggs and embryos inside cell-like compartments of defined sizes. Spindle size was observed to shrink with decreasing compartment size, similar to what occurs during early embryogenesis, and this scaling trend depended on compartment volume rather than shape. Thus, the amount of cytoplasmic material provides a mechanism for regulating the size of intracellular structures.

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Giant meiotic spindles in males from Drosophila species with giant sperm tails

Cited by 17 publications

References 36 publications

Hybrid male sterility and genome-wide misexpression of male reproductive proteases

Hybrid male sterility and genome-wide misexpression of male reproductive proteases

The Centrioles,Centrosomes, Basal Bodies, and Cilia of Drosophila melanogaster

Cytoplasmic Volume Modulates Spindle Size During Embryogenesis

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