Giant cell formation: the way to cell death or cell survival?

Horbay, Rostyslav; Stoika, Rostyslav

doi:10.2478/s11535-011-0058-0

Cited by 9 publications

(9 citation statements)

References 67 publications

(79 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies showed that polyploid giant cells have the ability to chromatid exchange and probably the production of a fully repaired chromosome. Moreover, homologous chromosomes from the giant cell can be relocated and paired which is the step to depolyploidization ( 47 , 48 ). Silencing of LMNB1 affects DNA damage and repair pathway.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of lamin B1 induces mitotic catastrophe in colon cancer LoVo cells and is associated with worse clinical outcomes

2017

View full text Add to dashboard Cite

Lamins are the major components of the nuclear lamina and play important roles in many cellular processes. The role of lamins in cancer development and progression is still unclear but it is known that reduced expression of lamin B1 has been observed in colon cancer. Thus, the aim of the present study was to elucidate the influence of LMNB1 upregulation on colon cancer cell line after treatment with 5-FU. The results indicate, that overexpression of LMNB1 induced dose-dependent cell death mainly by mitotic catastrophe pathway. Furthermore, after upregulation of this intermediate protein, lower expression of lamin A/C was observed. Moreover, we observed an increase in fluorescence intensity of nuclear β-catenin and decrease in cell-cell interaction area, that was connected with inhibition of colon cancer cells migration. We present the reorganization of actin filament and β-tubulin, because these cytoskeletal proteins are directly or indirectly linked with lamins, and analyzing publicly available mRNA data we show that patients with overexpression of LMNB1 are characterized by lower survival rates within the first 30 months from diagnosis. Summarizing our results, upregulation of LMNB1 induce mitotic catastrophe and only small percentage of apoptosis. Moreover, we showed inhibition of cell migration and promotion of cell-cell contact as a results of direct and indirect regulation of β-catenin, lamin A/C, actin and tubulin. However, it is possible that mitotic catastrophe cells in patients with colorectal cancer may be a reservoir of the cells responsible for faster disease progression, and further investigations are necessary to confirm this hypothesis.

show abstract

Section: Discussionmentioning

confidence: 99%

Overexpression of lamin B1 induces mitotic catastrophe in colon cancer LoVo cells and is associated with worse clinical outcomes

2017

View full text Add to dashboard Cite

show abstract

“…The giant cells exhibiting pleomorphism of the nuclei predicts bad prognosis of the tumour. [ 15 16 17 ]…”

Section: Giant Cellsmentioning

confidence: 99%

Uncommon features in conventional oral squamous cell carcinoma

Sudhakara

Venugopal

Khan

et al. 2016

J Oral Maxillofac Pathol

View full text Add to dashboard Cite

“…The relationship between PGCCs and senescent cells is still a matter of discussion, whereby also a standardized nomenclature is missing. PGCCs have been described as nondividing flattened tumor cells that are irreversibly arrested either in the G0/G1 or G2/M state and express β -galactosidase activity [ 8 , 9 ]. On the contrary, PGCCs have also been characterized as not senescent due to the lack of β -galactosidase staining [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells

Glassmann¹,

Garcia

Janzen

et al. 2018

Analytical Cellular Pathology

View full text Add to dashboard Cite

Cultivation of A549 non-small-cell lung carcinoma (NSCLC) cells in the presence of staurosporine (SSP) leads to a reduction or a lack of proliferation in a concentration-dependent manner. This inhibition of proliferation is accompanied by the generation of polyploid giant cancer cells (PGCCs) that are characterized by cell flattening, increased cell size, polyploidy, and polynucleation as determined by crystal violet staining, BrdU and DiI labelling, and flow cytometry as well as video time-lapse analysis. Continuous SSP treatment of A549 cells can preserve PGCCs for at least two months in a resting state. Upon removal of SSP, A549 PGCCs restart to divide and exhibit a proliferation pattern and cellular morphology indistinguishable from cells where PGCCs originally derived from. Thus, SSP-treated A549 cells represent a simple and reliable experimental model for the reversible generation of PGCCs and their subsequent experimental analysis.

show abstract

Giant cell formation: the way to cell death or cell survival?

Cited by 9 publications

References 67 publications

Overexpression of lamin B1 induces mitotic catastrophe in colon cancer LoVo cells and is associated with worse clinical outcomes

Overexpression of lamin B1 induces mitotic catastrophe in colon cancer LoVo cells and is associated with worse clinical outcomes

Uncommon features in conventional oral squamous cell carcinoma

Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells

Contact Info

Product

Resources

About