GHRH-SST-GH-IGF axis regulates crosstalk between growth and immunity in rainbow trout (Oncorhynchus mykiss) infected with Vibrio anguillarum

Hou, Zhi-Shuai; Xin, Yuan-Ru; Zeng, Chu; Zhao, Hong-Kui; Tian, Yuan; Li, Jifang; Wen, Haishen

doi:10.1016/j.fsi.2020.08.037

Cited by 10 publications

(13 citation statements)

References 97 publications

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“…Previous studies showed 10 7 to 10 9 CFU/ml of V. anguillarum could cause vibriosis in rainbow trout and other teleosts (5,(41)(42)(43). Our published paper further showed that V. anguillarum of 10 7 CFU/ml at 20°C exhibited mild to moderate symptoms of vibriosis disease with a relatively lower mortality (33). Therefore, trout were challenged by 10 7 CFU/ml of V. anguillarum at 20°C.…”

Section: Experimental Designsupporting

confidence: 55%

“…The bacterial suspension was then centrifuged and resuspended with 0.01 M phosphatebuffered saline (PBS, pH = 7.2). V. anguillarum suspension density was adjusted to serial dilutions for preliminary testing: 10 9 , 10 8 , or 10 7 colony forming units (CFU)/ml (33).…”

Section: Anguillarummentioning

confidence: 99%

“…This manuscript used the same RNA-Seq samples previously described in two papers evaluating the growth hormone and insulin-like growth factor axes, as well as the caspase gene family in rainbow trout (33,34). Previous studies showed 10 7 to 10 9 CFU/ml of V. anguillarum could cause vibriosis in rainbow trout and other teleosts (5,(41)(42)(43).…”

Section: Experimental Designmentioning

confidence: 99%

“…A total of 27 libraries [3 tissues (brain, kidney, spleen) × 3 replicated samples (each sample contained three pooled individuals × 3 treatment groups] was constructed via the TruSeq ™ RNA Sample Prep Kit (Illumina, CA, USA). This study used the same RNA-Seq data with our previously published paper (33,34), but we focused on different functional genes and used various analyses. The sequence reads are available from the NCBI sequence read archive (SRA) with the accession number of PRJNA667799.…”

Section: Rna-seq Analysismentioning

confidence: 99%

“…Rainbow trout (Oncorhynchus mykiss) is one of the most common aquaculture fish species worldwide (Food and Agriculture Organization of the United Nations); however, the trout industry is severely affected by vibriosis (27). In this study, the RNA-Seq datasets were retrieved from our previous studies (33,34), and we analyzed a total of 27 RNA-Seq libraries. Briefly, we investigated control trout (0.9% saline-injection), asymptomatic trout (AT; surviving trout with minor or no symptoms after V. anguillarum injection), and symptomatic trout (ST; moribund trout with severe symptoms after V. anguillarum injection).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional Profiles of Genes Related to Stress and Immune Response in Rainbow Trout (Oncorhynchus mykiss) Symptomatically or Asymptomatically Infected With Vibrio anguillarum

et al. 2021

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Rainbow trout (Oncorhynchus mykiss) is one of the most common aquaculture fish species worldwide. Vibriosis disease outbreaks cause significant setbacks to aquaculture. The stress and immune responses are bidirectionally modulated in response to the health challenges. Therefore, an investigation into the regulatory mechanisms of the stress and immune responses in trout is invaluable for identifying potential vibriosis treatments. We investigated the transcriptional profiles of genes associated with stress and trout immune functions after Vibrio anguillarum infection. We compared the control trout (CT, 0.9% saline injection), asymptomatic trout (AT, surviving trout with minor or no symptoms after bacteria injection), and symptomatic trout (ST, moribund trout with severe symptoms after bacteria injection). Our results showed activated immunomodulatory genes in the cytokine network and downregulated glucocorticoid and mineralocorticoid receptors in both AT and ST, indicating activation of the proinflammatory cytokine cascade as a common response in AT and ST. Moreover, the AT specifically activated the complement- and TNF-associated immune defenses in response to V. anguillarum infection. However, the complement and coagulation cascades, as well as steroid hormone homeostasis in ST, were disturbed by V. anguillarum. Our studies provide new insights toward understanding regulatory mechanisms in stress and immune functions in response to diseases.

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