Identification and characterization of caspases genes in rainbow trout (Oncorhynchus mykiss) and their expression profiles after Aeromonas salmonicida and Vibrio anguillarum infection

Transcriptional Profiles of Genes Related to Stress and Immune Response in Rainbow Trout (Oncorhynchus mykiss) Symptomatically or Asymptomatically Infected With Vibrio anguillarum

“…Moreover, the RNA-seq and qPCR data consistently showed the ST and AT exerted different expressions of genes in caspase family (34). For example, ST showed higher up-regulated casp8, which is involved in apoptosis regulation, pathogen detection and immunomodulation (34). In this study, based on multivariate analysis of PCA, significant differences in the transcriptional profiles of stress and immunerelated genes were observed in trout between the pairwise comparisons of CT, AT, and ST (Figures 2D-4D and Figure S1).…”

“…In brief, trout challenged by 10 7 CFU/ml of V. anguillarum began to die within 24 h after challenge and the mortality is around 20% within 120 h after challenge (33). Moreover, the RNA-seq and qPCR data consistently showed the ST and AT exerted different expressions of genes in caspase family (34). For example, ST showed higher up-regulated casp8, which is involved in apoptosis regulation, pathogen detection and immunomodulation (34).…”

“…This manuscript used the same RNA-Seq samples previously described in two papers evaluating the growth hormone and insulin-like growth factor axes, as well as the caspase gene family in rainbow trout (33,34). Previous studies showed 10 7 to 10 9 CFU/ml of V. anguillarum could cause vibriosis in rainbow trout and other teleosts (5,(41)(42)(43).…”

“…A total of 27 libraries [3 tissues (brain, kidney, spleen) × 3 replicated samples (each sample contained three pooled individuals × 3 treatment groups] was constructed via the TruSeq ™ RNA Sample Prep Kit (Illumina, CA, USA). This study used the same RNA-Seq data with our previously published paper (33,34), but we focused on different functional genes and used various analyses. The sequence reads are available from the NCBI sequence read archive (SRA) with the accession number of PRJNA667799.…”

“…Rainbow trout (Oncorhynchus mykiss) is one of the most common aquaculture fish species worldwide (Food and Agriculture Organization of the United Nations); however, the trout industry is severely affected by vibriosis (27). In this study, the RNA-Seq datasets were retrieved from our previous studies (33,34), and we analyzed a total of 27 RNA-Seq libraries. Briefly, we investigated control trout (0.9% saline-injection), asymptomatic trout (AT; surviving trout with minor or no symptoms after V. anguillarum injection), and symptomatic trout (ST; moribund trout with severe symptoms after V. anguillarum injection).…”