GhMPK17, a Cotton Mitogen-Activated Protein Kinase, Is Involved in Plant Response to High Salinity and Osmotic Stresses and ABA Signaling

Abstract

Mitogen-activated protein kinase (MAPK) cascades play pivotal roles in mediating biotic and abiotic stress responses. Cotton (Gossypium hirsutum) is the most important textile crop in the world, and often encounters abiotic stress during its growth seasons. In this study, a gene encoding a mitogen-activated protein kinase (MAPK) was isolated from cotton, and designated as GhMPK17. The open reading frame (ORF) of GhMPK17 gene is 1494 bp in length and encodes a protein with 497 amino acids. Quantitative RT-PCR analysis indicated that GhMPK17 expression was up-regulated in cotton under NaCl, mannitol and ABA treatments. The transgenic Arabidopsis plants expressing GhMPK17 gene showed higher seed germination, root elongation and cotyledon greening/expansion rates than those of the wild type on MS medium containing NaCl, mannitol and exogenous ABA, suggesting that overexpression of GhMPK17 in Arabidopsis increased plant ABA-insensitivity, and enhanced plant tolerance to salt and osmotic stresses. Furthermore, overexpression of GhMPK17 in Arabidopsis reduced H2O2 level and altered expression of ABA- and abiotic stress-related genes in the transgenic plants. Collectively, these data suggested that GhMPK17 gene may be involved in plant response to high salinity and osmotic stresses and ABA signaling.

RiceSUMOproteaseOverly Tolerant to Salt 1targets the transcription factor, OsbZIP23 to promote drought tolerance in rice

“…Furthermore, we analysed the transcript levels of selected drought responsive genes, which are known to be activated by ABA and by OsbZIP23 by qRT‐PCR in the empty vector control and transgenic lines (Figure (b–e)). The dehydrin Rab21 gene, which encodes a basic glycine‐rich protein (Mundy and Chua, ) belongs to a group of genes whose expression is regulated by PEG and ABA and is a target gene controlled by OsbZIP23 (Zhang et al ., ). Similarly, a group of hydrophilic proteins encoded by Late Embryogenesis Abundant (LEA) genes accumulate in plants during various stress conditions including drought and salinity suggesting a function in stress protection (Tunnacliffe and Wise, ).…”

Genome-wide analysis of transcription and photosynthesis inhibition in the harmful dinoflagellate Prorocentrum minimum in response to the biocide copper sulfate

“…Effects of excess copper on signal transduction pathway related genes Microarray analysis detected some signal transduction pathway related genes, including RAS family members and MAPK family members. In general, RAS and MAPK signaling pathways are found in most organisms, and they play important roles in controlling various process like cell migration, cell shape, cell proliferation and differentiation, apoptosis, survival, and motility, and are involved in various environmental stress responses (Sinha et al, 2011;Rasmussen et al, 2012;Zhang et al, 2014b). Copper has been suggested to have a function in RAS/MAPK pathway activation (Turski et al, 2012).…”

Genome-wide association reveals genetic variation of lint yield components under salty field conditions in cotton (Gossypium hirsutum L.)

“…Gh_A05G3239 encodes a peroxidase superfamily protein (POD), which has been shown to play an important role in anti-oxidation under salt stress in cotton [33]. Gh_ A08G1183 encodes a mitogen-activated protein kinase (MAPK), which has been widely reported to be associated with salt tolerance in cotton [34]. In addition to the ion transport GO term, carbohydrate metabolism was active under salt stress, for example, 10 genes were identified in the enriched GO term "polysaccharide catabolic process" and 3 of them were differentially expressed under salt stress.…”