Genome-wide analysis of transcription and photosynthesis inhibition in the harmful dinoflagellate Prorocentrum minimum in response to the biocide copper sulfate

Guo, Ruoyu; Lim, Weol‐Ae; Ki, Jang‐Seu

doi:10.1016/j.hal.2016.05.004

Cited by 27 publications

(2 citation statements)

References 85 publications

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“…The low percentage of DEGs in the assembled genes (4.92%) in this study was also consistent with previous research suggesting that regulation of gene expression may play a reduced role in dinoflagellates, which primarily rely on post-transcriptional regulation (Moustafa et al, 2010;Guo et al, 2016a;Guo et al, 2016b;Riaz et al, 2018;Wang et al, 2019). For instance, Wang et al ( 2019) demonstrated only 0.36% of total genes were differentially expressed in K. mikimotoi in responce to UV radiation.…”

Section: Transcriptomic Analysis Overviewsupporting

confidence: 92%

Transcriptome profiling reveals a global response in harmful dinoflagellate Karlodinium veneficum to naturally-occurring bacterial algicides

Wang

Coyne²

2023

Front. Mar. Sci.

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Dinoflagellates are among the most toxigenic phytoplankton that cause harmful algal blooms; they can produce toxins that accumulate through the aquatic food chains to cause illness and even death in marine animals and humans. Shewanella sp. IRI-160 is a naturally-occurring bacterium that secretes a suite of algicidal compounds (collectively designated as IRI-160AA) specifically targeting dinoflagellates. Studies revealed IRI-160AA inhibited photosynthesis, while inducing cell cycle arrest, DNA damage, and reactive oxygen species (ROS) production, as well as other markers associated with programmed cell death (PCD). Recent research indicated that IRI-160AA contains ammonium and other compounds as active ingredients for its algicidal activity, while impacts by ammonium differed from the algicide with respect to photobiology. Here, transcriptomic analysis was conducted on the toxic dinoflagellate Karlodinium veneficum exposed to IRI-160AA to investigate the effects of this algicide at the molecular level. Transcriptomic analysis was also conducted on K. veneficum treated with ammonium to further differentiate its effects from IRI-160AA. Results demonstrated differential impacts by IRI-160AA and ammonium on K. veneficum at the molecular level and revealed a global response of K. veneficum to algicide exposure, supported by the enriched biological processes involved in regulating gene expression, protein activity, and morphology. Differentially expressed genes associated with stress and ROS response, DNA damage response, cell cycle checkpoint activation, and PCD were also identified in K. veneficum exposed to IRI-160AA. The potential involvement of a recovery mechanism from photodamage in K. veneficum induced by IRI-160AA was identified. Overall, results of this study further differentiated the impacts of ammonium from IRI-160AA on K. veneficum and illustrated the cellular mechanisms behind the algicidal effect. This research provided insights on algal response to bacterial derivatives in nature.

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Section: Transcriptomic Analysis Overviewsupporting

confidence: 92%

Transcriptome profiling reveals a global response in harmful dinoflagellate Karlodinium veneficum to naturally-occurring bacterial algicides

Wang

Coyne²

2023

Front. Mar. Sci.

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“…The EC 50 of Korean C. ehren- the brown alga Padina boergesenii significantly declined in response to increasing Cu concentration and exposured time. Moreover, in chlorophytes Chlorella vulgaris, Pseudokirchneriella subcapitata, dinoflagellates Cochlodinium polykrikoides, and P. minimum exposed to 3 μM, 250 μM, 1.0 mg L -1 , and 0.5 mg L -1 of Cu, respectively, photosynthetic efficiency significantly declined in response to longer exposured times (Knauert and Knauer 2008, Guo et al 2016a, 2016b. Such results not only demonstrate that exposure of Cu has an adverse effect on photosynthetic efficiency in many organisms, but also that the Korean C. ehrenbergii is a particularly sensitive to exposure of Cu (see Table 1).…”

Section: Resultsmentioning

confidence: 99%

Physiological effects of copper on the freshwater alga Closterium ehrenbergii Meneghini (Conjugatophyceae) and its potential use in toxicity assessments

Wang

Sathasivam

2017

ALGAE

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Although green algae of the genus Closterium are considered ideal models for testing toxicity in aquatic ecosystems, little data about the effects of toxicity on these algal species is currently available. Here, Closterium ehrenbergii was used to assess the acute toxicity of copper (Cu). The median effective concentration (EC 50) of copper sulfate based on a dose response curve was 0.202 mg L-1 , and reductions in photosynthetic efficiency (F v / F m ratio) of cells were observed in cultures exposed to Cu for 6 h, with efficiency significantly reduced after 48 h (p < 0.01). In addition, production of reactive oxygen species significantly increased over time (p < 0.01), leading to damage to intracellular organelles. Our results indicate that Cu induces oxidative stress in cellular metabolic processes and causes severe physiological damage within C. ehrenbergii cells, and even cell death; moreover, they clearly suggest that C. ehrenbergii represents a potentially powerful test model for use in aquatic toxicity assessments.

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De novo transcriptome of the newly described phototrophic dinoflagellate Yihiella yeosuensis: comparison between vegetative cells and cysts

2019

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Genome-wide analysis of transcription and photosynthesis inhibition in the harmful dinoflagellate Prorocentrum minimum in response to the biocide copper sulfate

Cited by 27 publications

References 85 publications

Transcriptome profiling reveals a global response in harmful dinoflagellate Karlodinium veneficum to naturally-occurring bacterial algicides

Transcriptome profiling reveals a global response in harmful dinoflagellate Karlodinium veneficum to naturally-occurring bacterial algicides

Physiological effects of copper on the freshwater alga Closterium ehrenbergii Meneghini (Conjugatophyceae) and its potential use in toxicity assessments

De novo transcriptome of the newly described phototrophic dinoflagellate Yihiella yeosuensis: comparison between vegetative cells and cysts

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