GhBOP1 as a Key Factor of Ribosomal Biogenesis: Development of Wrinkled Leaves in Upland Cotton

Abstract

Block of proliferation 1 (BOP1) is a key protein that helps in the maturation of ribosomes and promotes the progression of the cell cycle. However, its role in the leaf morphogenesis of cotton remains unknown. Herein, we report and study the function of GhBOP1 isolated from Gossypium hirsutum. The sequence alignment revealed that BOP1 protein was highly conserved among different species. The yeast two-hybrid experiments, bimolecular fluorescence complementation, and luciferase complementation techniques revealed that GhBOP1 interact with GhPES and GhWDR12. Subcellular localization experiments revealed that GhBOP1, GhPES and GhWDR12 were localized at the nucleolus. Suppression of GhBOP1 transcripts resulted in the uneven bending of leaf margins and the presence of young wrinkled leaves by virus-induced gene silencing assay. Abnormal palisade arrangements and the presence of large upper epidermal cells were observed in the paraffin sections of the wrinkled leaves. Meanwhile, a jasmonic acid-related gene, GhOPR3, expression was increased. In addition, a negative effect was exerted on the cell cycle and the downregulation of the auxin-related genes was also observed. These results suggest that GhBOP1 plays a critical role in the development of wrinkled cotton leaves, and the process is potentially modulated through phytohormone signaling.

Identification of YUC genes associated with leaf wrinkling trait in Tacai variety of Chinese cabbage

“…Furthermore, a miRNA-targeted gene loss-of-function mutant, phb-6 (PHABULOSA, a member of the Class III HD-Zip family), displayed subtle bending phenotypes in leaves ( Liu et al, 2010 ). The GhBOP1 (Block of proliferation 1) gene, crucial in ribosome maturation and cell cycle progression, when silenced in upland cotton ( Gossypium hirsutum ) plants, resulted in the wrinkling phenotype of young leaves ( Wang et al, 2022b ). In Chinese cabbage, a stably inherited wrinkled leaf mutant named lcm was identified through EMS mutagenesis, with the candidate gene BraA01g007510.3C found to encode an H + ATPase 2 ( Zhang, 2020 ).…”

Comparative Genomics and Functional Genomics Analysis in Plants

“…Five papers included in the second portion are focused on plant growth and development. By studying gene expression data from upland cotton, Wang et al (2022) [ 8 ] identified a key factor in the growth and development of wrinkled cotton leaves and demonstrated that this process may be regulated by plant hormone signals. Gan et al (2022) [ 9 ] performed a genome-wide association study (GWAS) and a WGCNA of the genetic diversity of 91 wild bermudagrass resources.…”