Gga-miR-3525 Targets PDLIM3 through the MAPK Signaling Pathway to Regulate the Proliferation and Differentiation of Skeletal Muscle Satellite Cells

Abstract

MicroRNAs (miRNAs) are evolutionarily conserved, small noncoding RNAs that post-transcriptionally regulate expression of their target genes. Emerging evidence demonstrates that miRNAs are important regulators in the development of skeletal muscle satellite cells (SMSCs). Our previous research showed that gga-miR-3525 is differentially expressed in breast muscle of broilers (high growth rate) and layers (low growth rate). In this study, we report a new role for gga-miR-3525 as a myogenic miRNA that regulates skeletal muscle development in chickens. Exogenous increases in the expression of gga-miR-3525 significantly inhibited proliferation and differentiation of SMSCs, whereas the opposite effects were observed in gga-miR-3525 knockdown SMSCs. We confirmed that PDLIM3 (PDZ and LIM domain 3) is a target gene of gga-miR-3525 that can promote proliferation and differentiation of SMSCs. We found that PDLIM3 overexpression elevated the abundance of phosphorylated (p-)p38 protein but that the gga-miR-3525 mimic and p38-MAPK inhibitor (SB203580) weakened the activation of p-p38. Furthermore, treatment with SB203580 reduced the promoting effect of PDLIM3 on SMSC proliferation and differentiation. Overall, our results indicate that gga-miR-3525 regulates the proliferation and differentiation of SMSCs by targeting PDLIM3 via the p38/MAPK signaling pathway in chickens.

Exosomes Derived by SIRT1-Overexpressing Bone Marrow Mesenchymal Stem Cells Improve Pubococcygeus Muscle Injury in Rats

“…The molecular mechanisms underlying activation and proliferation of SCs involve interplay among multiple signaling pathways (35). Elia et al have reported that the activation and proliferation of SCs were mediated via ERK signaling pathway (36)(37)(38). Levels of ERK and p-ERK were examined in this study.…”

Comprehensive Analysis of PDLIM3 Expression Profile, Prognostic Value, and Correlations with Immune Infiltrates in Gastric Cancer

“…In spite of utilizing a variety of therapeutic options, gastric cancer patients are still suffering from unsatisfactory efficacies and high mortality rates. PDLIM3, a member of the ALP family, contributes to the formation of the Z line in mature muscle fibers and functions as a modulator of muscle cell activities such as cell differentiation, proliferation, signal transduction, stability, development, and cytoskeletal structural integration [ 13 ]. Previous research had revealed that PDLIM3 had carcinogenic potential, with higher expression levels being associated with a variety of malignancies and being relevant to poorer survival, chemotherapy resistance, and EMT [ 17 – 20 ].…”

“…It colocalizes with α -actinin participating in a component of the Z line of mature muscle fibers, highly expressed in cardiac, smooth, and skeletal muscle [ 12 ]. PDLIM3 not only has an essential role in cellular differentiation, proliferation, and signal transduction but also participates in muscle stability, development, and cytoskeletal structural integration [ 13 ]. In addition, it was reported that PDLIM3 is linked to myotonic dystrophy, muscular dystrophy, and DCM [ 11 , 14 , 15 ].…”

PDLIM5 Affects Chicken Skeletal Muscle Satellite Cell Proliferation and Differentiation via the p38-MAPK Pathway

“…In addition, PDZ and LIM domain 3 (PDLIM3) also belongs to the PDZ and LIM domain family as with PDLIM5. We found that PDLIM3 is the target gene of gga-miRNA-3525, and gga-miRNA-3525 can target PDLIM3 to inhibit the development of chicken SMSCs through the p38-MAPK pathway [ 32 ]. In addition, four and a half LIM domain protein 2 (FHL2) regulates the expression of interleukin-6 in muscle cells through the p38-MAPK signaling pathway [ 33 ].…”