Germline transmission of exogenous genes in chickens using helper-free ecotropic avian leukosis virus-based vectors

Thoraval, P.; Afanassieff, Marielle; Cosset, François‐Loïc; Lasserre, Frédèric; Verdier, Gérard; Coudert, F; Dambrine, Ginette

doi:10.1007/bf01973755

Cited by 53 publications

(29 citation statements)

References 40 publications

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“…Efforts have mainly involved either the injection of retroviral vectors into embryos at the blastodermal stage (1,7,8,24,26,32,35,36,42,43) or the microinjection of DNA into fertilized eggs at the single-cell stage (21, 39). Recently, lentiviral vectors were also used to generate transgenic chickens (3, 23).…”

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confidence: 99%

High-Level Expression of Single-Chain Fv-Fc Fusion Protein in Serum and Egg White of Genetically Manipulated Chickens by Using a Retroviral Vector

Kamihira

Ono

Esaka

et al. 2005

J Virol

122

112

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We report here the generation of transgenic chickens using a retroviral vector for the production of recombinant proteins. It was found that the transgene expression was suppressed when a Moloney murine leukemia virus-based retroviral vector was injected into chicken embryos at the blastodermal stage. When a concentrated viral solution was injected into the heart of developing embryos after 50 to 60 h of incubation, transgene expression was observed throughout the embryo, including the gonads. For practical production, a retroviral vector encoding an expression cassette of antiprion single-chain Fv fused with the Fc region of human immunoglobulin G1 (scFv-Fc) was injected into chicken embryos. The birds that hatched stably produced scFv-Fc in their serum and eggs at high levels (ϳ5.6 mg/ml). We obtained transgenic progeny from a transgenic chicken generated with this procedure. The transgene was stably integrated into the chromosomes of transgenic progeny. The transgenic progeny also expressed scFv-Fc in the serum and eggs.Transgenic bioreactors possess great potential for the production of recombinant pharmaceutical proteins (10,15,34). Transgenic animals have been generated for the production of recombinant proteins in the milk of livestock species such as goats, sheep, pigs, and cows. A high level of expression, more than 1 mg/ml of milk, has been reported with some proteins, and efforts toward a practical application for pharmaceutical use have continued (15). However, mammalian systems have several drawbacks in that they require a relatively large area for breeding and a long period for sexual maturation. As an alternative transgenic bioreactor, avian species such as chickens and quails have attracted a great deal of attention (11, 37). In particular, chickens have several advantages, including high protein productivity in eggs, ease of and small space requirements for breeding, similarity of the glycosylation pattern of proteins to that of humans (31), and absence of the prion problem.To date, many approaches have attempted to generate transgenic birds (38). Efforts have mainly involved either the injection of retroviral vectors into embryos at the blastodermal stage (1,7,8,24,26,32,35,36,42,43) or the microinjection of DNA into fertilized eggs at the single-cell stage (21, 39). Recently, lentiviral vectors were also used to generate transgenic chickens (3, 23). In most previous studies, a reporter gene such as lacZ and GPF has been used as the target, and expression was limited within cells. Harvey et al. reported that ␤-lactamase was produced in the serum and egg white of transgenic chickens generated with an avian leukosis virus-based retroviral vector (7,8). As a practical model for the production of a pharmaceutical protein, human alpha-2b interferon was produced using the same system (32). However, the expression levels in serum and eggs were not high enough compared with the mammalian transgenic bioreactor systems.Because of their availability, laid fertilized eggs at the blastodermal stage (stage X...

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confidence: 99%

High-Level Expression of Single-Chain Fv-Fc Fusion Protein in Serum and Egg White of Genetically Manipulated Chickens by Using a Retroviral Vector

Kamihira

Ono

Esaka

et al. 2005

J Virol

122

112

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“…However, the previous investigators (Vick et al, 1993) never demonstrated beta-galactosidase expression in the offspring. Transgenic chickens carrying the lacZ gene were also produced using a replication-defective avian leukosis based retroviral vector (Thoraval et al, 1995). Unfortunately, beta-galactosidase expression was only noted in cultures of embryonic fibroblasts from G2 progeny, and expression was not reported in the entire embryo.…”

Section: Retroviral Historical Perspectivementioning

confidence: 99%

Status of transgenic chicken models for developmental biology

Mozdziak

Petitte

2004

Developmental Dynamics

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The chick embryo is a classic model that has been used to gain insight into developmental processes and cell fate within the embryo for over a century. For the most part, investigators have implanted quail cells into a chicken embryo. A more powerful tool for developmental biology research than the quail:chick chimera system would be to have lines of transgenic chickens expressing reporter genes that are readily available to the research community. However, avian transgenic technology has been fraught with technical difficulties, and transgenic chickens expressing reporter genes have only recently been developed. The goal of this review is to report the technologies that have been used to generate transgenic chickens and to discuss the challenges in generating avian transgenics for developmental biology research. Developmental Dynamics 229:414 -421, 2004.

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“…The generation of transgenic chickens has been reported by several groups [49][50][51][52][53][54][55][56][57][58][59]. In the 1980s, bird replication-competent retroviral (RCR) vectors were developed and used for the generation of transgenic chickens [49,50].Although the viral vectors could be amplified in infected cells, the germ-line transmission efficiency was as low as 10%.…”

Section: Transgenic Birds For the Production Of Recombinant Proteins mentioning

confidence: 99%

Transgenic Birds for the Production of Recombinant Proteins

Kamihira

Nishijima

Iijima

2004

Recent Progress of Biochemical and Biomedical Engineering in Japan II

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Germline transmission of exogenous genes in chickens using helper-free ecotropic avian leukosis virus-based vectors

Cited by 53 publications

References 40 publications

High-Level Expression of Single-Chain Fv-Fc Fusion Protein in Serum and Egg White of Genetically Manipulated Chickens by Using a Retroviral Vector

High-Level Expression of Single-Chain Fv-Fc Fusion Protein in Serum and Egg White of Genetically Manipulated Chickens by Using a Retroviral Vector

Status of transgenic chicken models for developmental biology

Transgenic Birds for the Production of Recombinant Proteins

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