Transgenic Birds for the Production of Recombinant Proteins

Kamihira, Masamichi; Nishijima, Ken ichi; Iijima, Shinji

doi:10.1007/b94209

Cited by 6 publications

(4 citation statements)

References 63 publications

(97 reference statements)

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“…In their method, more than 43% quail embryos hatched when calcium lactate was supplemented in the culture. This system corresponded to the Perry's system III and the culture method was also applied to chick embryos (Kamihira et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels

Tahara¹,

Obara²

2014

J. Poult. Sci.

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The development of shell-less culture methods for bird embryos with high hatchability would be useful for the efficient generation of transgenic chickens, embryo manipulations, tissue engineering, and basic studies in regenerative medicine. To date, studies of culture methods for bird embryos include the whole embryo culture using narrow windowed eggshells, surrogate eggshells, and an artificial vessel using a gas-permeable membrane. However, there are no reports achieving high hatchability of ＞50% using completely artificial vessels. To establish a simple method for culturing chick embryos with high hatchability, we examined various culture conditions, including methods for calcium supplementation and oxygen aeration. In the embryo cultures where the embryos were transferred to the culture vessel after 55-56 h incubation, more than 90% of embryos survived until day 17 when a polymethylpentene film was used as a culture vessel with calcium lactate and distilled water supplementations. The aeration of pure oxygen to the surviving embryos from day 17 yielded a hatchability of 57.1% (8 out of 14). Thus, we successfully achieved a high hatchability with this method in chicken embryo culture using an artificial vessel.

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Section: Introductionmentioning

confidence: 99%

A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels

Tahara¹,

Obara²

2014

J. Poult. Sci.

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“…Chickens have several merits over mammals including their rapid maturation, ease of and small space requirement for breeding, and daily high protein production in eggs (Kamihira et al 2004). Since many therapeutics are glycoproteins that at least partly require N-glycosylation for their biological functions, the ability for Nglycosylation is a basic requirement for these production systems.…”

Section: Introductionmentioning

confidence: 99%

Recombinant proteins produced into yolk of genetically manipulated chickens are partly sialylated in N-glycan

et al. 2013

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The transgenic chicken is a candidate for the production of biopharmaceutical proteins with several economic superiorities. In general, the addition of sialic acid at the terminal of N-glycan is important for the bioactivity of biopharmaceuticals including plasma half-life; however, sialic acid has not been detected in the N-glycan of proteins produced in the egg white of genetically manipulated chickens. In this study, the extracellular domain of the TNF receptor and single chain Fv fused to Fc (referred to as TNFR/Fc and scFv/Fc, respectively) were purified from the egg yolk of genetically manipulated chickens and their sialylation in N-glycan was examined. In contrast to the glycan in egg white, yolk-derived proteins were partly sialylated. Lectin blot showed the existence of a2,6-sialic acid on TNFR/Fc, which disappeared with the removal of N-glycan by PNGase. In scFv/Fc, up to 7 % of N-glycan contained sialic acid. Disialyl glycans, which were detected in serumderived scFv/Fc in a previous study, were not found in the yolk sample. Ovarian follicular tissue, which surrounds growing yolk, expressed several neuraminidases, suggesting the partial truncation of glycan during the yolk transfer process from the blood.

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“…The injection of a retroviral vectors into chicken embryonic hearts also resulted in ubiquitous transgene expression throughout the body, including the gonads (48). In addition to chicken, transgenesis by retroviral construct infection has been attempted (49) and achieved (14,50,51) in Japanese quails. Further advances in avian stem cell biology allow for techniques other than the use of viral vectors, such as germline transmission of primordial germ cells (PGC) (52).…”

Section: Use Of Viral Vectors In Introducing Genes 219mentioning

confidence: 99%

“…Retroviral pseudotypes are engineered viruses in which the retroviral genome is encapsidated by the envelope protein of a second virus, such as VSV. Because VSV has a very broad host range, retroviruses pseudotyped with VSV-G could infect crustaceans (54,55), mollusks (53), mammals (22,29), fish (58,61,84) and birds (14,15,47,48,51). In addition, a MoMLV vector has been pseudotyped with Gibbon ape leukemia virus (GaLV) envelope protein and used to infect goat mammary glands resulting in the secretion of therapeutic proteins by mammary glands (11).…”

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confidence: 99%

The Use of Viral Vectors in Introducing Genes into Agricultural Animal Species

Modric

Mergia

2009

Animal Biotechnology

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The use of viral vectors is a method for introducing foreign genes into various animal species. Vectors based on retro-, adeno-, flavi-, and parvoviruses have been used for research in animal species of agricultural importance, such as chickens, quail, swine, cows, goats, sheep, fish, crustaceans, and mollusks. Viral vectors allow for efficient transgenic integration into host genome or for transient expression of the transgenic construct in somatic tissues. Because of that, viral vectors are important tools for research and potentially other biotechnology applications such as improving animal production qualities and introducing disease resistance, thus improving food quality and safety. Other uses may include generating animal models of human diseases and using animals as bioreactors for production of therapeutic proteins. Each vector type provides a unique set of advantages and limitations, which are in some cases specific to an animal species or a method of introduction. This article discusses viral vector characteristics and potential applications in agriculturally important animal species. It discusses advantages and disadvantages of using viral vectors in genetic engineering of agricultural animals.

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Transgenic Birds for the Production of Recombinant Proteins

Cited by 6 publications

References 63 publications

A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels

A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels

Recombinant proteins produced into yolk of genetically manipulated chickens are partly sialylated in N-glycan

The Use of Viral Vectors in Introducing Genes into Agricultural Animal Species

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