Germline transformation of the diamondback moth, Plutella xylostella L., using the piggyBac transposable element

Martins, Sara; Naish, Neil; Walker, Adam; Morrison, Neil I.; Scaife, Sarah; Fu, Guoliang; Dafa'alla, Tarig; Alphey, Luke

doi:10.1111/j.1365-2583.2012.01146.x

Cited by 64 publications

(56 citation statements)

References 47 publications

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“…The total cost connected with the damage and the management of DBM was previously estimated at 4–5 billion USD per annum worldwide 2 . Much research has been devoted to the DBM, covering pest management strategies 3,4 , the mechanism of pesticide resistance 5–7 , its developmental biology 8–10 , and herbivory mechanism 11 . However, DBM outbreaks in Southeast Asia sometimes cause more than a 90% crop loss, and this pest still seriously threatens vegetable production in China.…”

Section: Introductionmentioning

confidence: 99%

Midgut bacteria in deltamethrin-resistant, deltamethrin-susceptible, and field-caught populations of Plutella xylostella, and phenomics of the predominant midgut bacterium Enterococcus mundtii

Jin

Shi

et al. 2017

Sci Rep

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Gut bacteria play a significant role in host insect. This study evaluated detail difference of midgut bacteria in deltamethrin-resistant, deltamethrin-susceptible and field-caught populations of diamondback moth, and studied phenomics of the predominant midgut bacterium Enterococcus mundtii. Cultivable bacteria revealed that E. mundtii and Carnobacterium maltaromaticum dominated the bacterial populations from deltamethrin-resistant and deltamethrin-susceptible larval midguts, whereas E. mundtii was predominant in field-caught population. Illumina sequencing analysis indicated that 97% of the midgut bacteria were from the phyla Firmicutes, Proteobacteria and Cyanobacteria. Both resistant and susceptible populations had more Enterococcus and Carnobacterium. Enterococcus, Carnobacterium, Bacillus, and Pseudomonas were predominant in the field-caught population. A phenomics analysis revealed that E. mundtii was able to metabolize 25.26% of the tested carbon sources, 100% of the nitrogen sources, 100% of the phosphorus sources and 97.14% of the sulfur sources, had a wide range of osmolytes and pH conditions, and showed active deaminase activity but no decarboxylase activity. This is the first report regarding different populations of DBM midgut bacteria analyzed using both high-throughput DNA sequencing and cultivation methods, and also first report concerning the phenomics of E. mundtii. The phenomics of E. mundtii provide a basis for the future study of gut bacteria functions.

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Section: Introductionmentioning

confidence: 99%

Midgut bacteria in deltamethrin-resistant, deltamethrin-susceptible, and field-caught populations of Plutella xylostella, and phenomics of the predominant midgut bacterium Enterococcus mundtii

Jin

Shi

et al. 2017

Sci Rep

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“…The nonautonomous piggyBac vector allows germ-line transformation of insects via microinjection with separately supplied piggyBac transposase. The fluorescent marker is DsRed2 under the control of a baculovirus-derived promoter, Hr5ie1; this provides strong red fluorescence in a range of insect species (25,29,33,34). The conditional female-specific gene is designed to derive its lethal effect via hyperaccumulation of tTAV from a positive feedback loop, leading to transcriptional suppression (25,26).…”

Section: Resultsmentioning

confidence: 99%

Transgene-based, female-specific lethality system for genetic sexing of the silkworm, Bombyx mori

Tan

Jin

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

120

140

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Transgene-based genetic sexing methods are being developed for insects of agricultural and public health importance. Male-only rearing has long been sought in sericulture because males show superior economic characteristics, such as better fitness, lower food consumption, and higher silk yield. Here we report the establishment of a transgene-based genetic sexing system for the silkworm, Bombyx mori. We developed a construct in which a positive feedback loop regulated by sex-specific alternative splicing leads to high-level expression of the tetracycline-repressible transactivator in females only. Transgenic animals show female-specific lethality during embryonic and early larval stages, leading to male-only cocoons. This transgene-based female-specific lethal system not only has wide application in sericulture, but also has great potential in lepidopteran pest control.Lepidoptera | doublesex T he mulberry silkworm, Bombyx mori, is a completely domesticated insect and is the foundation of sericulture, an endeavor of great economic importance. It is believed that sericulture originated in China and has been conducted there for more than 5,000 y (1). Male-only rearing techniques for B. mori are desirable because males show higher resistance to disease, lower food consumption, and better silk quality (2). To this end, several B. mori strains for male-only rearing have been developed by classical genetics. In the last century, Strunnikov (3) established a sex-linked, balanced-lethal system using radiation-induced chromosome translocations. However, conventional approaches involving selective breeding or irradiation for developing male-only silkworm strains are time and labor consuming. Thus, novel approaches are desired to improve modern silkworm breeding. In recent years, advances in B. mori genetic manipulation, notably genetic transformation, have been successfully established and applied extensively in gene function analysis and the production of bioreactors (1, 4-7). These technologies provide a potential basis for improvements in sericulture including the development of a male-only rearing system.Transgene-based genetic sexing systems have been developed in Drosophila melanogaster (8, 9) and several medically and agriculturally important insect species (10-13) as part of a series of genetics-based improvements and alternatives to the sterile insect technique (14). Systems based on sex-specific lethality for improving silk production are preferable to those using differential expression of a marker gene, for example fluorescence, which then would require manual or automated examination of each individual as part of the sorting process. Molecular designs developed for Diptera should be able to provide genetic sexing in B. mori, as the ability to transfer systems from one species to another is a key advantage of transgenic approaches over classical genetic methods (14-16). Furthermore, despite early reports to the contrary, more recent work has established clearly that transgenic strains can be developed with good...

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“…Rearing of diamondback moth and olive fly followed procedures described by Martins et al. () and Ant et al. (), respectively.…”

Section: Methodsmentioning

confidence: 99%

Population‐level effects of fitness costs associated with repressible female‐lethal transgene insertions in two pest insects

Harvey‐Samuel

Ant

Gong

et al. 2014

Evolutionary Applications

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Genetic control strategies offer great potential for the sustainable and effective control of insect pests. These strategies involve the field release of transgenic insects with the aim of introducing engineered alleles into wild populations, either permanently or transiently. Their efficacy can therefore be reduced if transgene-associated fitness costs reduce the relative performance of released insects. We describe a method of measuring the fitness costs associated with transgenes by analyzing their evolutionary trajectories when placed in competition with wild-type alleles in replicated cage populations. Using this method, we estimated lifetime fitness costs associated with two repressible female-lethal transgenes in the diamondback moth and olive fly as being acceptable for field suppression programs. Furthermore, using these estimates of genotype-level fitness costs, we were able to project longer-term evolutionary trajectories for the transgenes investigated. Results from these projections demonstrate that although transgene-associated fitness costs will ultimately cause these transgenes to become extinct, even when engineered lethality is repressed, they may persist for varying periods of time before doing so. This implies that tetracycline-mediated transgene field persistence in these strains is unlikely and suggests that realistic estimates of transgene-associated fitness costs may be useful in trialing ‘uncoupled’ gene drive system components in the field.

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Germline transformation of the diamondback moth, Plutella xylostella L., using the piggyBac transposable element

Cited by 64 publications

References 47 publications

Midgut bacteria in deltamethrin-resistant, deltamethrin-susceptible, and field-caught populations of Plutella xylostella, and phenomics of the predominant midgut bacterium Enterococcus mundtii

Midgut bacteria in deltamethrin-resistant, deltamethrin-susceptible, and field-caught populations of Plutella xylostella, and phenomics of the predominant midgut bacterium Enterococcus mundtii

Transgene-based, female-specific lethality system for genetic sexing of the silkworm, Bombyx mori

Population‐level effects of fitness costs associated with repressible female‐lethal transgene insertions in two pest insects

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