Germinação in vitro de Hyptis leucocephala Mart. ex Benth. e Hyptis platanifolia Mart. ex Benth.

Abstract

RESUMO:Hyptis leucocephala e Hyptis platanifolia (Lamiaceae) são espécies aromáticas endêmicas do semiárido nordestino e possuem grande importância econômica devido ao seu potencial medicinal. O Palavras-chave: Meios de cultura, Estabelecimento in vitro, Esterilização química, Crescimento in vitro.ABSTRACT: In vitro germination of Hyptis leucocephala Mart. ex Benth. and Hyptis platanifolia Mart. ex Benth. The Hyptis leucocephala and Hyptis platanifolia (Lamiaceae) are aromatic species, endemic to the Brazilian Semiarid northeast, and they have great economic importance becaude of their pharmacological potential. This study aimed to obtain the protocol for the in vitro establishment of H. leucocephala and H. platanifolia plants. The WPM promoted higher germination percentage (96.81%) for H. leucocephala, but there was no difference between the results obtained in ½MS, in the last week of cultivation. For the germination of H. platanifolia, ½MS was the one that provided the highest percentage (40.83%). The type of sterilization did not affect the in vitro germination of both species. For the H. platanifolia species, when we closed the tubes with a cotton plug, hyperhydricity was eliminated, but the percentage of seed germination was the lowest one. Evaluating the type of culture medium on the in vitro growth of H. leucocephala, it did not influence the number of shoots, length of shoot or root dry matter. The MS medium promoted an increase of the number of leaves, dry shoot and longest root length, and the ½MS medium was more suitable for root induction. The optimal culture medium for the germination of H. leucocephala and H. platanifolia is ½MS, as it can be sterilized with sodium hypochlorite, while the MS was the best culture medium for the in vitro growth of H. leucocephala.

Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium

“…The use of active chlorine for the chemical sterilization of culture medium aiming to the in vitro propagation of plants has already been proven for several species (RIBEIRO et al, 2011;BRONDANI et al, 2013;NEPOMUCENO et al, 2014); however it was not recorded in literature for C. regium. Explants were transferred at each subculture, to the same culture medium composition but with different sterilization preparations such as autoclaving, 0.001%, 0.003%, and 0.005% Cl.…”

“…Autoclaving is an energetically expensive operation but is the most commonly sterilization method used in the laboratory. Chemical sterilization of culture medium with sodium hypochlorite (NaOCl) is an alternative method for asepsis of lower cost that has been used for in vitro culture of several species, such as Sequoia sempervirens (RIBEIRO et al, 2011), Chrysantemum (DEEIN et al, 2013), Eucalyptus benthamii (BRONDANI et al, 2013), Hyptis leucochepala and H. platanifolia (NEPOMUCENO et al, 2014). Nevertheless, the necessary conditions for the active chlorine not to cause damage to the growth and development of C. regium tissues plant are not established.…”

In vitro growth optimization and essential oil composition of basil cultivars

“…agropec. bras., Brasília, v.59, e03478, 2024 DOI: 10.1590/S1678-3921.pab2024.v59.03478 medium, responsible for improving ionic strength and better meeting the nutritional requirements of the cultivars (Neponuceno et al, 2014). Since it resulted in a better general plant performance, the MS medium was used in the following sequential experiments.…”

Association between Cuttings Maturity and Alternative Substrates in the Rooting of Acerola Cherry

“…Vegetative propagation is based on the capacity of part of the plant to regenerate from somatic cells [9] such as the rooting of cuttings. This faculty depends on two basic characteristics: totipotence and differentiation [10,11], and implies the mitotic division of cells, in which there is a duplication in the chromosome and cytoplasm system [12].…”