Germinação de pólen e aplicação de ácido bórico em botões florais de nespereiras

Nogueira, Paulyene Vieira; Silva, Daniel Fernandes da; Pio, Rafael; Silva, Pedro Augusto de Oliveira; Bisi, Rayane Barcelos; Balbi, Rodrigo Vieira

doi:10.1590/1678-4499.0264

Cited by 15 publications

(8 citation statements)

References 19 publications

(20 reference statements)

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“…Five anthers were then separated at random, and each set of anthers stored in uncapped Eppendorf tubes at a controlled temperature (27 °C) for 24 h in the absence of light, for dehiscence to take place with the release of pollen grains (NOGUEIRA et al, 2015). After 24 hours, a solution of 1,000 µl of lactic acid was added to the tube.…”

Section: Quantification Of Pollen Grains Per Anther and Per Flowermentioning

confidence: 99%

Establishment of growth medium and quantification of pollen grains and germination of pear tree cultivars

Nogueira

Coutinho

Pio

et al. 2016

Revista Ciência Agronômica

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Section: Quantification Of Pollen Grains Per Anther and Per Flowermentioning

confidence: 99%

Establishment of growth medium and quantification of pollen grains and germination of pear tree cultivars

Nogueira

Coutinho

Pio

et al. 2016

Revista Ciência Agronômica

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“…Similar results were observed when using calcium nitrate in the in vitro germination of pollen grains of peach and pear trees (Chagas et al 2009). Similarly, according to Nogueira et al (2014), the culture medium absent of calcium nitrate promoted more germination of loquat pollen grains.…”

Section: Resultsmentioning

confidence: 90%

“…This was most likely related to a gradual increase in the solute concentration of the medium that disrupted the integrity of the pollen grain cell structure (Dantas et al 2005). The application of boric acid in the field raised the pollen grain germination of loquat (Nogueira et al 2014), which proves the need for boric acid in pollen grain germination.…”

Section: Resultsmentioning

confidence: 97%

“…Thus, the high percentage of germination under increased sucrose concentrations may be explained by the increased energy supplied in the form of carbohydrates, favouring the growth of the pollen tube. A study of pollen germination of blackberry and loquat concluded that the highest germination rates were achieved at the highest concentrations of sucrose added to the medium (Figueiredo et al 2013;Nogueira et al 2014).…”

Section: Resultsmentioning

confidence: 99%

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Establishment of growth medium and quantification of pollen grains of olive cultivars in Brazil's subtropical areas

et al. 2015

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“…On the other hand, in analyzing Eriobotrya japonica, Nogueira et al (2015) found a linear increase in the pollen germination percentage as the agar concentration increased. The same behavior was observed in relation to the addition of sucrose to the culture medium.…”

Section: Discussionmentioning

confidence: 96%

ANTIOXIDANTS, SUCROSE AND AGAR IN THE IN VITRO MULTIPLICATION OF Eremanthus incanus

Miranda

Titon

Pereira

et al. 2018

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The objective of this study was to evaluate the in vitro multiplication of Eremanthus incanus axillary buds in the presence of PVP additives and activated charcoal under the interaction of different sucrose and agar concentrations. Explants containing axillary buds were inoculated in MS culture medium to evaluate the effect of 0.8 g L -1 of PVP and 1.0, 2.0 and 4.0 g L -1 of activated charcoal. The results of the addition of 0, 15, 30, and 60 g L -1 of sucrose combined with concentrations of 6 and 10 g L -1 of agar in MS medium were also evaluated. The number and quality of emitted shoots were evaluated after 30 and 60 days. The addition of PVP to the culture medium was more efficient than the use of activated charcoal in the multiplication of the species. The presence of sucrose is indispensable for developing the shoots well, and the use of a lower concentration of agar favors the development of quality shoots. The best results for shoot multiplication were obtained using 30 g L -1 sucrose and 6 g L -1 agar. PVP, activated charcoal, agar and sucrose are important components in the culture medium and alter the in vitro multiplication of Eremanthus incanus. Keywords: Tissue culture, micropropagation, culture medium. ResumoAntioxidantes, sacarose e ágar na multiplicação in vitro de Eremanthus incanus. O objetivo deste estudo foi avaliar a multiplicação in vitro de gemas axilares de Eremanthus incanus na presença dos aditivos polivinilpirrolidona -PVP e carvão ativado, e a interação de diferentes concentrações de sacarose e ágar. Os explantes que continham gemas axilares foram inoculados em meio de cultura MS para avaliar o efeito de 0,8 g L -1 de PVP e 1,0, 2,0 e 4,0 g L -1 de carvão ativado. Avaliou-se também o resultado da adição de 0, 15, 30 e 60 g L -1 de sacarose, combinado com concentrações de 6 e 10 g L -1 de ágar em meio MS. Após 30 e 60 dias, foram avaliados o número e a qualidade das brotações emitidas. A adição de PVP ao meio de cultura foi mais eficiente que o uso de carvão ativado na multiplicação da espécie. A presença de sacarose é indispensável para o bom desenvolvimento das brotações, assim como o uso de menor concentração de ágar favorece o desenvolvimento de brotos de qualidade. Os melhores resultados para a multiplicação das gemas foram obtidos utilizando 30 g L -1 de sacarose e 6 g L -1 de ágar. PVP, carvão ativado, ágar e sacarose são componentes importantes no meio de cultura e alteram a multiplicação in vitro de Eremanthus incanus. Palavras-chave: Cultura de tecidos, micropropagação, meio de cultura.

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Germinação de pólen e aplicação de ácido bórico em botões florais de nespereiras

Abstract: of boric acid on field raised the pollen grain germination to 57.68%.

Cited by 15 publications

References 19 publications

Establishment of growth medium and quantification of pollen grains and germination of pear tree cultivars

Establishment of growth medium and quantification of pollen grains and germination of pear tree cultivars

Establishment of growth medium and quantification of pollen grains of olive cultivars in Brazil's subtropical areas

ANTIOXIDANTS, SUCROSE AND AGAR IN THE IN VITRO MULTIPLICATION OF Eremanthus incanus

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