Germin like protein genes exhibit modular expression during salt and drought stress in elite rice cultivars

Anum, Jazba; O'Shea, Charlotte; Farrukh, Sumaira; Skriver, Karen; Malik, Saad Imran; Yasmin, Tayyaba

doi:10.1007/s11033-021-06871-3

Cited by 15 publications

(8 citation statements)

References 33 publications

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“… 17 A similar expression was also shown by OsGLP8-4 , OsGLP8-7 , and OsGLP8-12 in two rice varieties (Super Basmati and KS282) largely mediated by methylation of specific cytosine residues in the promoter of OsGLPs. ( 105 ) In another study, GLP was highly accumulated in the leaves of Boea hygrometrica against drought. 14 In the case of auxin, no observable response was observed where ZmGLP4-2 , ZmGLP4-3 , ZmGLP4-4 , ZmGLP4-8 , ZmGLP4-13 , ZmGLP4-15 , ZmGLP10-1 , ZmGLP10-2 , ZmGLP10-3 , and ZmGLP10-4 showed moderate expression toward callus induction on auxin-less medium and in embryo samples, but ZmGLP2-1 showed the highest expression.…”

Section: Resultsmentioning

confidence: 96%

Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

Ilyas¹,

Ali

Binjawhar

et al. 2023

ACS Omega

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Germin (GER) and germin-like proteins (GLPs) play an important role in various plant processes. Zea mays contains 26 germin-like protein genes ( ZmGLPs ) located on chromosomes 2, 4, and 10; most of which are functionally unexplored. The present study aimed to characterize all ZmGLPs using the latest computational tools. All of them were studied at a physicochemical, subcellular, structural, and functional level, and their expression was predicted in plant development, against biotic and abiotic stresses using various in silico approaches. Overall, ZmGLPs showed greater similarity in their physicochemical properties, domain architecture, and structure, mostly localized in the cytoplasmic or extracellular regions. Phylogenetically, they have a narrow genetic background with a recent history of gene duplication events on chromosome 4. Functional analysis revealed novel enzymatic activities of phosphoglycolate phosphatase, adenosylhomocysteinase, phosphoglycolate phosphatase-like, osmotin/thaumatin-like, and acetohydroxy acid isomeroreductase largely mediated by disulfide bonding. Expression analysis revealed their crucial role in the root, root tips, crown root, elongation and maturation zones, radicle, and cortex with the highest expression being observed during germination and at the maturity levels. Further, ZmGLPs showed strong expression against biotic ( Aspergillus flavus , Colletotrichum graminicola , Cercospora zeina , Fusarium verticillioides , and Fusarium virguliforme ) while limited expression was noted against abiotic stresses. Concisely, our results provide a platform for additional functional exploration of the ZmGLP genes against various environmental stresses.

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Section: Resultsmentioning

confidence: 96%

Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

Ilyas¹,

Ali

Binjawhar

et al. 2023

ACS Omega

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“…Low-temperature stress can cause serious damage to melon, resulting in the decline of melon yield and quality, thus restricting the development of the melon industry [ 40 , 41 ]. GLPs play an active role in plants coping with abiotic stresses, including low-temperature stress [ 42 , 43 , 44 ]. Based on the analysis of the cis-acting elements of the promoters of the melon GLP gene family, the promoters of the members of the melon GLP gene family contained a large number of DSREs, including low-temperature responsiveness elements ( Figure 4 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification, Characterization, and Expression Analysis Related to Low-Temperature Stress of the CmGLP Gene Family in Cucumis melo L.

Zhang

Wen

Yuan

et al. 2022

IJMS

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Germin-like protein (GLP) participates in plant growth and development and plays an important role in plant stress. In the present study, 22 CmGLPs belonging to five classes were identified in the melon genome. Each member of the CmGLPs family contains a typical Cupin_1 domain. We conducted a genome-wide analysis of the melon GLP gene family characterization. CmGLPs were randomly distributed in the melon chromosomes, with the largest number on chromosome 8, having eight family members. Gene duplication events drive the evolution and expansion of the melon GLP gene family. Based on the phylogenetic tree analysis of GLP proteins in melon, rice, Arabidopsis, and cucumber, it was found that the GLP gene families of different species have diverged in evolution. Based on qRT-PCR results, all members of the CmGLP gene family could be expressed in different tissues of melon. Most CmGLP genes were up-regulated after low-temperature stress. The relative expression of CmGLP2-5 increased by 157.13 times at 48 h after low-temperature treatment. This finding suggests that the CmGLP2-5 might play an important role in low-temperature stress in melon. Furthermore, quantitative dual LUC assays indicated that CmMYB23 and CmWRKY33 can bind the promoter fragment of the CmGLP2-5. These results were helpful in understanding the functional succession and evolution of the melon GLP gene family and further revealed the response of CmGLPs to low-temperature stress in melon.

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“…Plant GLP gene s also play vital roles in regulating biotic and abiotic stress responses. Numerous studies have demonstrated that the GLPs are widely involved in resistance to diverse pathogens such as S. sclerotiorum ( Rietz et al, 2012 ; Zhang et al, 2018 ), Rhizoctonia solani ( Beracochea et al, 2015 ), Blumeria graminis ( Yuan et al, 2021 ), Magnaporthe oryzae ( Liu et al, 2016 ), Aspergillus flavus ( Wang et al, 2013 ), and response to UV-B radiation ( He et al, 2021 ), heat ( Gangadhar et al, 2021 ; Zaynab et al, 2021 ), drought ( Wang et al, 2013 ; Anum et al, 2022 ), heavy metal ( Cheng et al, 2018 ) and wound ( Wang et al, 2013 ). To further explore the possible function of BnCDPs in stress resistance, the analysis of cis -acting regulatory elements in the promoter regions was conducted in this study.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, overexpression of StGLP in potato plants increased the H 2 O 2 level, triggered the scavenging signaling pathways of reactive oxygen species and induced the expression of heat stress-responsive genes to enhance the tolerance to heat stress ( Gangadhar et al, 2021 ). Besides, the GLP genes are also responsive to other abiotic stresses such as drought ( Anum et al, 2022 ), heavy metal ( Cheng et al, 2018 ) and wound ( Wang et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and functional analysis of cupin_1 domain-containing members involved in the responses to Sclerotinia sclerotiorum and abiotic stress in Brassica napus

Bai

et al. 2022

Front. Plant Sci.

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Cupin_1 domain-containing proteins (CDPs) are ubiquitously present in higher plants, which are known to play essential roles in various biological processes. In this study, we carried out genome-wide characterization and systematic investigation of the CDP genes in Brassica napus. A total of 96 BnCDPs, including 71 germin-like proteins (GLPs; proteins with a single cupin_1 domain) and 25 CDP bicupins (proteins with two cupin_1 domains), were identified and clustered into six distinct subfamilies (I–VI) based on the phylogenic analysis, gene structure and motif distribution. Further analysis indicated that whole-genome duplication (WGD) and segmental duplication are main contributors to the species-specific expansion of the BnCDP gene family, and all the duplicated genes subsequently underwent strong purification selection. The promoter region of BnCDPs showed enrichment of cis-regulatory elements associated with development, hormone and stress, as well as transcription factor binding sites, which validates the prediction that BnCDPs are widely involved in plant growth and biotic and abiotic stress responses. The BnCDPs in different subfamilies exhibited obvious differences in expression among 30 developmental tissues/stages of B. napus, implying that BnCDPs may be involved in tissue- and stage-specific developmental processes. Similar trends in expression of most BnCDPs were observed under Sclerotinia sclerotiorum inoculation and four abiotic stresses (dehydration, cold, ABA and salinity), particularly the BnGLPs in subfamily I and III with single cupin_1 domain, revealing that BnCDPs are of great importance in the environmental adaption of B. napus. We then performed a genome-wide association study (GWAS) of 274 B. napus core germplasms on S. sclerotiorum resistance and identified four significantly associated loci harboring five BnGLPs. The expression levels of two candidate genes, BnGLP1.A08 and BnGLP1.C08, were significantly correlated with S. sclerotiorum resistance. Their functional responses to multiple stages of S. sclerotiorum inoculation and four abiotic stresses were further examined through qPCR. Overall, this study provides rich resources for research on the function and evolutionary playground of CDP genes.

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Germin like protein genes exhibit modular expression during salt and drought stress in elite rice cultivars

Cited by 15 publications

References 33 publications

Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

Genome-Wide Identification, Characterization, and Expression Analysis Related to Low-Temperature Stress of the CmGLP Gene Family in Cucumis melo L.

Genome-wide identification and functional analysis of cupin_1 domain-containing members involved in the responses to Sclerotinia sclerotiorum and abiotic stress in Brassica napus

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