Germ-tube production by <i>Candida albicans</i> in minimal liquid culture media

Dn, Mardon; Sk, Hurst; Balish, Edward

doi:10.1139/m71-137

Cited by 32 publications

(15 citation statements)

References 8 publications

(7 reference statements)

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“…In addition there is a requirement for a nitrogen source and for salts. The nitrogen sources used have included various peptones ; L-asparagine, provided that serum albumin was also present (Bernander & Edebo, 1969); serum and serum dialysates (Taschdjian & Kozinn, 1961); mixtures of amino acids (Taschdjian & Kozinn, 1961;Johnson, Guzmon & Agurlera, 1954; Mycelium induction in C. albicans 321 Balish & Phillips, 1969; Dabrowa, 1971;Lee, Buckley & Campbell, 1975); single amino acids such as glycine (De Palma, 1966), proline (Dabrowa, 1971) and L-a-amino-n-butyrate (Mardon, Hurst & Balish, 1971); and ammonium sulphate (Chattaway, Odds & Barlow, 1971;Evans et al, 1974;Marriott, 1975). It is difficult to compare the various findings and to reach any general conclusions because of variations in the other factors mentioned above, differences in the method of assessing the extent of mycelial development and the fact that often only a single strain has been examined.…”

Section: Discussionmentioning

confidence: 99%

Induction of the Mycelial Form of Candida albicans by Hydrolysates of Peptides from Seminal Plasma

Chattaway

O’Reilly

Barlow

et al. 1976

Journal of General Microbiology

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SUMMARYPrevious work led to the separation from seminal plasma of a peptide fraction which promoted a high rate of germination of blastospores of Candida albicans. It has now been shown that an acid hydrolysate of this material is also highly active. A minimal amino-acid mixture consisting of aspartic acid, lysine, histidine, threonine, proline and, p-alanine gave go % germination in 4 h with 17 out of 28 strains examined. Glucose and inorganic phosphate were also required. Phosphate was not required for the activity of the original peptide fraction.

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Section: Discussionmentioning

confidence: 99%

Induction of the Mycelial Form of Candida albicans by Hydrolysates of Peptides from Seminal Plasma

Chattaway

O’Reilly

Barlow

et al. 1976

Journal of General Microbiology

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“…A dimorphic process starts in Candida albicans when a blastospore produces a short filament, called the 'germ or pseudogerm tube' (Hill & Gebhardt, 1956;Taschdjian & Kozinn, 1957;Mackenzie, 1962;Mardon, Hurst & Balish, 1971), which can later evolve into a true mycelium (Mackenzie, 1964;Simonetti & Cassone, 1969). Germ-tube production is of a great value for laboratory diagnosis of C. albicans (Taschdjian, Burchall & Kozinn, 1960;Mackenzie, 1962;Buckley & Van Uden, 1963).…”

Section: N T R O D U C T I O Xmentioning

confidence: 99%

Ultrastructural Changes in the Wall during Germ-tube Formation from Blastospores of Candida albicans

Cassone¹,

Simonetti²,

Strippoli³

1973

Journal of General Microbiology

119

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“…(2) Vaginal material inoculated on to Sabouraud's medium was incubated at 37°C., colonies of C. albicans being identified by the author by the "germ tube" technique (Mackenzie, 1962;Mardon, Hurst, and Balish, 1971).…”

Section: Methodsmentioning

confidence: 99%

Non-specific genital infection in a general practice.

Fox¹

1974

Sexually Transmitted Infections

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The rising incidence of male urethral infection of uncertain aetiology is at present a major problem. The reported figures for non-gonococcal urethritis in England and Wales increased in the 20 years from 1951 to 1970 by nearly 38,000, most of these cases being of the non-specific variety. There are epidemiological reasons for thinking that non-specific urethritis (NSU) has its counterpart in women as nonspecific urogenital infection (King, 1972). For notification purposes, the condition in both sexes is now referred to as non-specific genital infection (NSGI) with, or without, arthritis. In England in 1971 the venereal disease clinic returns for "NSGI without arthritis" totalled 59,023 for men and 13,397 for women (Department of Health and Social Security, 1972).The difficulty in defining the clinical disorder in women has contributed to the problem (Catterall, 1971;Department of Health and Social Security, 1970; Brit. med. J., 1971a;Nicol, 1971 Inflammatory disease of the cervix is often seen in the course of routine examination of women in general practice. In the present investigation an attempt has been made to relate this to possible unsuspected NSU in their male consorts. In addition, the effect of the condom in reducing the frequency of NSGI has been studied. Material and methodsThis study was conducted in a predominantly workingclass practice in London. During the investigation 325 women were studied; 80 per cent. were in their third or fourth decade and 95 per cent. were married. 98 women (30 per cent.) were examined because they complained of urogenital symptoms; 227 women (70 per cent.) presented without symptoms and were examined routinely, e.g. for post-natal check, contraception, and periodic cervical cytology. Couples, either partner of whom had had systemic antibiotics within 3 months of examination, and pregnant women have been excluded from this report.When these 325 women were examined, it was explained that tests involving both sexual partners would be done "to exclude inflammation". In every case women were asked about a past history of genital disorders and for details of recent sexual contact and contraceptive technique.Clinical examination With the woman in the dorsal position, the external genitalia were examined and cleansed and the vagina and cervix inspected via a Cusco's speculum. Specimens were collected as follows: (1) A scrape from the squamo-columnar junction of the cervix was spread with an Ayre's spatula on a glass slide and fixed for cytology.(2) Vaginal, cervical, urethral, and rectal specimens were spread on glass slides; further specimens from the vagina, cervix, and occasionally the urethra and rectum

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Germ-tube production by Candida albicans in minimal liquid culture media

Cited by 32 publications

References 8 publications

Induction of the Mycelial Form of Candida albicans by Hydrolysates of Peptides from Seminal Plasma

Induction of the Mycelial Form of Candida albicans by Hydrolysates of Peptides from Seminal Plasma

Ultrastructural Changes in the Wall during Germ-tube Formation from Blastospores of Candida albicans

Non-specific genital infection in a general practice.

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