1971
DOI: 10.1139/m71-137
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Germ-tube production by Candida albicans in minimal liquid culture media

Abstract: Candida albicans formed germ tubes within 3 h at 37C in a glucose–salts–biotin (GSB) medium containing L-alpha-amino-n-butyric acid as the nitrogen source. Optimal germ-tube production was obtained when the inoculum was grown on Sabouraud dextrose agar. The GSB medium containing L-alpha-amino-n-butyric acid promoted germ-tube formation more effectively than GSB medium plus gamma-amino-butyric acid or Sabouraud dextrose broth.Carbon-14 incorporation studies revealed that during germ-tube formation (0–4 h) the 3… Show more

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Cited by 32 publications
(15 citation statements)
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References 8 publications
(7 reference statements)
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“…In addition there is a requirement for a nitrogen source and for salts. The nitrogen sources used have included various peptones ; L-asparagine, provided that serum albumin was also present (Bernander & Edebo, 1969); serum and serum dialysates (Taschdjian & Kozinn, 1961); mixtures of amino acids (Taschdjian & Kozinn, 1961;Johnson, Guzmon & Agurlera, 1954; Mycelium induction in C. albicans 321 Balish & Phillips, 1969; Dabrowa, 1971;Lee, Buckley & Campbell, 1975); single amino acids such as glycine (De Palma, 1966), proline (Dabrowa, 1971) and L-a-amino-n-butyrate (Mardon, Hurst & Balish, 1971); and ammonium sulphate (Chattaway, Odds & Barlow, 1971;Evans et al, 1974;Marriott, 1975). It is difficult to compare the various findings and to reach any general conclusions because of variations in the other factors mentioned above, differences in the method of assessing the extent of mycelial development and the fact that often only a single strain has been examined.…”
Section: Discussionmentioning
confidence: 99%
“…In addition there is a requirement for a nitrogen source and for salts. The nitrogen sources used have included various peptones ; L-asparagine, provided that serum albumin was also present (Bernander & Edebo, 1969); serum and serum dialysates (Taschdjian & Kozinn, 1961); mixtures of amino acids (Taschdjian & Kozinn, 1961;Johnson, Guzmon & Agurlera, 1954; Mycelium induction in C. albicans 321 Balish & Phillips, 1969; Dabrowa, 1971;Lee, Buckley & Campbell, 1975); single amino acids such as glycine (De Palma, 1966), proline (Dabrowa, 1971) and L-a-amino-n-butyrate (Mardon, Hurst & Balish, 1971); and ammonium sulphate (Chattaway, Odds & Barlow, 1971;Evans et al, 1974;Marriott, 1975). It is difficult to compare the various findings and to reach any general conclusions because of variations in the other factors mentioned above, differences in the method of assessing the extent of mycelial development and the fact that often only a single strain has been examined.…”
Section: Discussionmentioning
confidence: 99%
“…A dimorphic process starts in Candida albicans when a blastospore produces a short filament, called the 'germ or pseudogerm tube' (Hill & Gebhardt, 1956;Taschdjian & Kozinn, 1957;Mackenzie, 1962;Mardon, Hurst & Balish, 1971), which can later evolve into a true mycelium (Mackenzie, 1964;Simonetti & Cassone, 1969). Germ-tube production is of a great value for laboratory diagnosis of C. albicans (Taschdjian, Burchall & Kozinn, 1960;Mackenzie, 1962;Buckley & Van Uden, 1963).…”
Section: N T R O D U C T I O Xmentioning
confidence: 99%
“…(2) Vaginal material inoculated on to Sabouraud's medium was incubated at 37°C., colonies of C. albicans being identified by the author by the "germ tube" technique (Mackenzie, 1962;Mardon, Hurst, and Balish, 1971).…”
Section: Methodsmentioning
confidence: 99%