Germ line transmission of autonomous genetic elements in transgenic mouse strains

Rassoulzadegan, Minoo; Léopold, Pierre; Vailly, Joëlle; Cuzin, François

doi:10.1016/0092-8674(86)90876-7

Cited by 58 publications

(23 citation statements)

References 20 publications

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“…In previous studies, however, the existence of integration hotspots had been proposed in some cases. DNA topoisomerase I or II binding sites were found to cluster around the junctions; short, purine-rich tracts, some short forward and reverse overlapping sequences or short, direct repeats consisting of 4-6 bp, ATrich S/MAR were present, either at the junction site or in the immediate flanking regions [15,[26][27][28][29][30]. In the present study, similar results were found.…”

Section: Analysis Of Integration Sitessupporting

confidence: 85%

“…However, up to the present, comprehensive information about the status of the transgene copies in F4 transgenic fish has been lacking. Since the technique of plasmid rescue was firstly described by Perucho et al [13]), it has been utilized in the study of transgenic mice [14][15][16][17], transgenic tomato [18] and transgenic Drosophila [19], but it has rarely, to our knowledge, been employed previously in the study of transgenic fish. Recently, we briefly reported three integration-site sequences in F4 transgenic fish [20]; however, the detailed integration pattern of transgene in F4 transgenic fish needs to be clarified.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of transgene integration pattern in F4 hGH-transgenic common carp (Cyprinus carpio L.)

Sun

Wang

et al. 2005

Cell Res

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The integration pattern and adjacent host sequences of the inserted pMThGH-transgene in the F4 hGH-transgenic common carp were extensively studied. Here we show that each F4 transgenic fish contained about 200 copies of the pMThGH-transgene and the transgenes were integrated into the host genome generally with concatemers in a head-totail arrangement at 4-5 insertion sites. By using a method of plasmid rescue, four hundred copies of transgenes from two individuals of F4 transgenic fish, A and B, were recovered and clarified into 6 classes. All classes of recovered transgenes contained either complete or partial pMThGH sequences. The class I, which comprised 83% and 84.5% respectively of the recovered transgene copies from fish A and B, had maintained the original configuration, indicating that most transgenes were faithfully inherited during the four generations of reproduction. The other five classes were different from the original configuration in both molecular weight and restriction map, indicating that a few transgenes had undergone mutation, rearrangement or deletion during integration and germline transmission. In the five types of aberrant transgenes, three flanking sequences of the host genome were analyzed. These sequences were common carp β-actin gene, common carp DNA sequences homologous to mouse phosphoglycerate kinase-1 and human epidermal keratin 14, respectively.

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Section: Analysis Of Integration Sitessupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Characterization of transgene integration pattern in F4 hGH-transgenic common carp (Cyprinus carpio L.)

Sun

Wang

et al. 2005

Cell Res

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“…It has been also observed that, when plasmid DNAs are introduced, they often exist extrachromosomally [8,13,14]. For the expression and transmission, the introduced plasmids need not be integrated; expression and transmission of episomal forms has been reported in mice [33,34]. In the present experiment, however, the founder which incorporated MT/hGHR gene fragment could transmit the exogenous DNA up to F4 generation so far, indicating that the gene was stably integrated into the germ line.…”

Section: Discussionmentioning

confidence: 51%

Production of Transgenic Rats and Mice by the Testis-Mediated Gene Transfer

Chang

Ikeda

Hayashi

et al. 1999

Journal of Reproduction and Development

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Abstract. Recent reports have shown that sperm cells incubated with foreign DNA in vitro are able to transfer the DNA into eggs at fertilization. The present study examined if an injection of DNA into the testis in vivo could generate transgenic animals via sperm ejaculated. We prepared 3 gene constructs; human growth hormone (hGH), hGH receptor (hGHR) and mouse leptin (mOB) genes fused to the promoter regions of the mouse genes for whey acidic protein (WAP), metallothionein-I (MT) and mouse mammary tumor virus (MMTV), respectively. Each gene construct mixed with cationic liposome was injected into bilateral testes of male rats or mice, and the males mated with females 3 or 4 days later. A female rat mated with a male treated with MT/hGHR gene gave birth to 17 pups, 3 of which were found to carry the transgene. The expression of hGHR mRNA was demonstrated in the liver, kidney, muscle and brain after treating with zinc in drinking water. At present, the transmission of the exogenous gene to the descendants was confirmed up to the F4 generation. Three female rats mated with 3 different males injected with MMTV/mOB fusion gene produced 10, 14 and 12 pups, respectively. The genome of 2 out of these 36 pups harbored MMTVmOB gene, though expression of mOB mRNA was not detected. Two female mice were mated with 2 male mice injected with WAP/hGH gene and produced 15 and 16 pups, 3 of which incorporated the gene. The expression of hGH mRNA in the mammary gland in these mice was confirmed. These results indicate that exogenous DNA injected into the testis as a liposome-complex can be transferred into eggs via sperm and expressed in the postpartum progeny.

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“…All appeared positive but the bands were just visible, suggesting a decrease in copy number from the Fo generation. This high frequency of transmission for episomal elements was observed also by Rassoulzadegan et al (14). It implies that the copy number of the episome in germinal cells must be close to one per cell.…”

supporting

confidence: 49%

Episomal maintenance of a bovine papilloma virus vector in transgenic mice.

Elbrecht¹,

DeMayo²,

Tsai³

et al. 1987

Mol. Cell. Biol.

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We have used a bovine papillomavirus-based vector to generate transgenic mice. Transgenic mice result from either pronuclear or cytoplasmic injections of the vector into fertilized eggs. Of 30 mice generated by microinjection, 27 (90%) contained the vector in its episomal form, at less than one copy per cell. This represents a highly efficient means of gene transfer in which the transgene is in a controlled genetic environment.

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Germ line transmission of autonomous genetic elements in transgenic mouse strains

Cited by 58 publications

References 20 publications

Characterization of transgene integration pattern in F4 hGH-transgenic common carp (Cyprinus carpio L.)

Characterization of transgene integration pattern in F4 hGH-transgenic common carp (Cyprinus carpio L.)

Production of Transgenic Rats and Mice by the Testis-Mediated Gene Transfer

Episomal maintenance of a bovine papilloma virus vector in transgenic mice.

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