Geometric models for calculating cell biovolume and surface area for phytoplankton

Sun, Jun; Liu, Dongyan

doi:10.1093/plankt/fbg096

Cited by 931 publications

(560 citation statements)

References 33 publications

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“…Micrasterias, Euastrum and some Staurastrum species. In these taxa, application of classical geometric formulas approximating surfaces and volumes of cells as simple geometric objects (Hillebrand et al, 1999;Sun & Liu, 2003) may not be appropriate. While the geometric formulas for calculation of S/V ratios and biovolumes have often been successfully applied in phytoplankton studies (e.g.…”

Section: Discussionmentioning

confidence: 99%

Diversity and morphological disparity of desmid assemblages in Central European peatlands

2009

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Morphological disparity has increasingly been used as an alternative measure of biological diversity based on the shape features of organisms. In this study, we investigated the species diversity and morphological disparity of benthic Desmidiales in Central European peatland pools. The shape features of cells were determined using the 3-D elliptical Fourier analysis of their frontal and lateral views. The resulting morphospace was used to calculate the contributions of localities and species to the morphological variation. In addition, the disparity of samples and their average cell complexity (indicating intricacy of cell shapes) was evaluated. These data were related to species diversity data and to the abiotic factors. Species diversity was positively correlated with pH and conductivity. The low-pH localities generally supported a more variable species composition than did slightly acidic to neutral localities.Conversely, the total nitrogen concentrations of these areas negatively correlated with species diversity. Interestingly, partial morphological disparity (measuring the contribution of a sample to the overall morphological variation) did not correlate with species diversity. On the contrary, several mountain peat bog localities had high disparity values, irrespective of their rather low species diversity. In addition, several samples from minerotrophic fens with high diversity had average or low values of partial morphological disparity. These results indicate the relative importance of mountain peat bogs for the total morphological diversity of Desmidiales within the region that could not be ascertained solely from species diversity data. The inner morphological disparity of samples was highly correlated with their species diversity. Species of the genus Micrasterias, Hyalotheca dissiliens and Desmidium species had the highest partial morphological disparity, thus indicating their marginal position within the morphospace. Micrasterias and Euastrum species had the highest complexity values. The average cell complexity of individual samples did not correlate with their diversity or disparity; however, it was positively correlated with the levels of total nitrogen and phosphorus, and illustrates a pattern different from that arrived at by species diversity data.

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Section: Discussionmentioning

confidence: 99%

Diversity and morphological disparity of desmid assemblages in Central European peatlands

2009

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“…This is extremely important since plankton biomass is calculated based on the size of planktonic organisms. The abundance of algae was determined using the method of Utermöhl (1958), in which their biomass is measured volumetrically (Hillebrand et al 1999;Sun and Liu 2003), assuming that 1 mm 3 of algae is equal to 1 mg (Elser and Carpenter 1988). To assess the similarity between phytoplankton species composition, the Jaccard's coefficient was calculated using MVSP 3.2 software (Kovach 2010).…”

Section: Methodsmentioning

confidence: 99%

Understanding Abiotic and Biotic Conditions in Post-Mining Pit Lakes for Efficient Management: A Case Study (Poland)

et al. 2017

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“…O limite de contagem foi estabelecido de acordo com curva de rarefação de espécies e até atingir o total de 100 indivíduos da espécie mais comum (Bicudo 1990). O biovolume algal foi obtido pela multiplicação da densidade de cada espécie pelo volume médio de suas células considerando, sempre que possível, a dimensão média de 20 indivíduos de cada espécie , Sun & Liu 2003. Com base na densidade dos táxons foram determinados os índices de diversidade de Shannon-Wiener (bits ind -1 ), dominância, equitatividade e riqueza de espécies (Krebs 1999).…”

Section: Methodsunclassified

Efeito de diferentes níveis de enriquecimento por fósforo sobre a estrutura da comunidade perifítica em represa oligotrófica tropical (São Paulo, Brasil)

Ferragut¹,

Bicudo²

2009

Rev. bras. Bot.

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-(Effect of different levels of phosphorus enrichment on periphyton community structure in a tropical oligotrophic reservoir (São Paulo, Brazil)). Nutrient diffusing substrata (NDS) were used to determine the effect of added phosphorus on the periphyton development in a shallow reservoir (Lago do IAG, PEFI, São Paulo). Three treatments (n = 2) were designed: control (without phosphorous addition) and two levels of phosphorus addition (P 1 = 0.1 M and P 2 = 0.5 M KH 2 PO 4 ). NDS surface was covered with a 20 μm mesh cloth for periphyton growth. Samplings were carried at 15, 20, 25 and 30 days of colonization. Biomass accumulation (organic matter, chlorophyll-a, total algal biovolume) and total algal density did not show signifi cant response to nutrient addition. Seven species of chlorophytes and one of diatoms were closely associated to P availability. Periphyton nutrient status (P%, N%, N:P) demonstrated P-limitation as well as reinforced periphyton P retention capacity. Periphyton attributes were more sensible to P enrichment than to the different addition levels, indicating further N-limitation. Comparison with previous data indicated temporal (1996)(1997)(1998)(1999)(2000)(2001)(2002) increase in the reservoir phosphorus availability, although P-limitation is still prevalent. Periphyton, on the basis of its nutrient status and species structure, was sensible to temporal shift on phosphorus availability and P-addition, reinforcing its potential as an evaluating tool for early signs of eutrophication.Key words -algae, environmental descriptors, periphyton, phosphorus, reservoir RESUMO -(Efeito de diferentes níveis de enriquecimento por fósforo sobre a estrutura da comunidade perifítica em represa oligotrófi ca tropical (São Paulo, Brasil)). Substrato difusor de nutrientes (SDN) foi utilizado para determinar o efeito da adição de fósforo sobre o desenvolvimento do perifíton em uma represa rasa (Lago do IAG, PEFI, São Paulo). Três tratamentos (n = 2) foram delineados: controle (sem adição de fósforo) e dois com adição de fósforo (P 1 = 0,1 M e P 2 = 0,5 M KH 2 PO 4 ). A superfície dos SDN foi revestida com malha de 20 μm para crescimento do perifíton. Coletas foram realizadas nos 15 o , 20 o , 25 o e 30 o dias de colonização. A acumulação de biomassa (massa orgânica, clorofi la-a, biovolume total de algas) e de densidade total de algas não forneceu resposta signifi cativa ao enriquecimento. Seis espécies de clorofíceas e uma de diatomáceas associaram-se à disponibilidade de fósforo. O estado nutricional do perifíton (%P, %N, N:P) demonstrou a limitação pelo fósforo, bem como reforçou a capacidade da comunidade na retenção do fósforo. Os atributos do perifíton foram mais sensíveis ao enriquecimento pelo fósforo, do que aos níveis de adição, indicando que a comunidade passou a ser limitada pelo nitrogênio. A comparação com dados anteriores indicou aumento temporal (1996-2002) da disponibilidade de fósforo na represa, embora ainda prevalecendo condição P-limitante. O perifíton, por meio de seu estado nutr...

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Geometric models for calculating cell biovolume and surface area for phytoplankton

Cited by 931 publications

References 33 publications

Diversity and morphological disparity of desmid assemblages in Central European peatlands

Diversity and morphological disparity of desmid assemblages in Central European peatlands

Understanding Abiotic and Biotic Conditions in Post-Mining Pit Lakes for Efficient Management: A Case Study (Poland)

Efeito de diferentes níveis de enriquecimento por fósforo sobre a estrutura da comunidade perifítica em represa oligotrófica tropical (São Paulo, Brasil)

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