Geometric alignment and chromatic calibration of serial radiographic images

Dornier, Christophe; Dorsaz-Brossa, L; Thévenaz, P.; Brochut, P.; Mombelli, Andrea; Vallée, Jean‐Paul

doi:10.1259/dmfr/71716997

Cited by 9 publications

(8 citation statements)

References 18 publications

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“…), and the mean pixel values were evaluated in interdental alveolar bone (from the ABC to the root apex) using I magej software (NIH, Bethesda, MD, USA), as previously described (33). Before being used to make the measurements, I magej software was calibrated and tested using radiographs of an aluminium wedge comprising a set of standard gray values, as previously described (34). This calibration was repeated in the analysis of all radiographs with the same wedge in identical positions throughout the same gray values.…”

Section: Methodsmentioning

confidence: 99%

Down‐regulation of expression of osteoblast and osteocyte markers in periodontal tissues associated with the spontaneous alveolar bone loss of interleukin‐10 knockout mice

Claudino

Garlet

Cardoso

et al. 2010

European J Oral Sciences

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The aim of this study was to unravel the mechanisms by which interleukin (IL)-10, a potent pleiotropic cytokine, modulates alveolar bone homeostasis in C57BL/6 wild-type (WT) and IL-10 knockout (IL-10 KO) mice, evaluated at 8, 24, and 48 wk of age. Interleukin-10 KO mice presented significant alveolar bone loss when compared with WT mice, and this was not associated with changes in leukocyte counts or bacterial load. The levels of expression of messenger RNA (mRNA) for tumor necrosis factor-alpha (TNF-alpha), IL-1beta, IL-6, transforming growth factor-beta (TGF-beta), receptor activator of nuclear factor kappaB ligand (RANKL), osteoprotegerin (OPG), and matrix metalloproteinase 13 (MMP13) were similar between both strains, whereas a significant decrease of tissue inhibitor of metalloproteinase 1 (TIMP1) mRNA expression was found at 48 wk in IL-10 KO mice. The osteoblast markers core binding factor alpha1 (CBFA1) and type I collagen (COL-I) were expressed at similar levels in both strains, whereas the levels of alkaline phosphatase (ALP) and osteocalcin (OCN), and those of the osteocyte markers phosphate-regulating gene endopeptidases (PHEX) and dentin matrix protein 1 (DMP1) were significantly lower in IL-10 KO mice. Our results demonstrate that the alveolar bone loss in the absence of IL-10 was associated with a reduced expression of osteoblast and osteocyte markers, an effect independent of microbial, inflammatory or bone-resorptive pathways.

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Section: Methodsmentioning

confidence: 99%

Down‐regulation of expression of osteoblast and osteocyte markers in periodontal tissues associated with the spontaneous alveolar bone loss of interleukin‐10 knockout mice

Claudino

Garlet

Cardoso

et al. 2010

European J Oral Sciences

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“…A number of studies have used exposures of the aluminum step wedge model as a reference to evaluate bone density [9,11,15] or monitoring bone changes [11,14,16]. All of these methods have reported accuracy in recording evaluating changes in bone.…”

Section: Discussionmentioning

confidence: 99%

“…Graf et al [20] designed a simple paralleling instrument for superposition of the molar regions. Dornier et al [21] used this position device to minimize the projection divergence in his study. In our study, a wooden box was utilized to minimize geometric variation of the exposures.…”

Section: Discussionmentioning

confidence: 99%

Development of a clinically applicable tool for bone density assessment

et al. 2008

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“…The use of an X-ray wedge calibration tool has also been proposed by some researchers groups (Bolander et al, 1990;De Josselin de Jong et al, 1989;Dornier et al, 2004;Escarpinati et al, 2002;Haidekker et al, 2004;Martin et al, 1990;Pearson et al, 2002;Saiki et al, 1993;Symmons, 2004) as a suitable tool to normalize radiographs. Nevertheless up to now in the orthopaedic scenario the assessment of bone tissue is still monitored by using the single energy X-ray exam, where no additional device is incorporated during the routine protocol.…”

Section: Introductionmentioning

confidence: 99%

A simple non invasive computerized method for the assessment of bone repair within osteoconductive porous bioceramic grafts

Beltrame

Cancedda

Canesi

et al. 2005

Biotechnol. Bioeng.

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Single energy X-ray imaging, due to its low cost and flexibility, is one of the most used and common technique to assess bone state and bone remodeling over time. Standardized X-ray images are needed to compare sets of radiographs for semi-quantitative analyses of tissue remodeling. However, useful mathematical modeling for the analysis of high level radiographic images are not easily available. In order to propose a useful evaluation tool to a wide clinical scenario, we present an innovative calibration algorithm for a semi-quantitative analysis of non-standardized digitized X-ray images. For calibration on a unique standardization scale, three time invariant regions (ROI) of radiographs were selected and analyzed. The accuracy of the normalization method for X-ray films was successfully validated by using an aluminum step wedge for routine X-ray exposures as tool to standardize serial radiographs (Pearson correlation test: R(2) = 0.96). This method was applied to investigate the progression of the new bone deposition within ceramic scaffolds used as osteoconductive substitute in large bone defects taking advantage of a large animal model. This innovative image-processing algorithm allowed the identification and semi-quantification of the bone matrix deposited within the implant. The osteo-integration at the bone-implant interface was also investigated. A progressively increasing bone tissue deposition within the porous bioceramic implant and a progressive osteo-integration was observed during the 12 months of the trial.

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Geometric alignment and chromatic calibration of serial radiographic images

Abstract: Very user-friendly software was developed. The manipulator needs to scan the radiographs only one time. The software performs all subsequent processing steps.

Cited by 9 publications

References 18 publications

Down‐regulation of expression of osteoblast and osteocyte markers in periodontal tissues associated with the spontaneous alveolar bone loss of interleukin‐10 knockout mice

Down‐regulation of expression of osteoblast and osteocyte markers in periodontal tissues associated with the spontaneous alveolar bone loss of interleukin‐10 knockout mice

Development of a clinically applicable tool for bone density assessment

A simple non invasive computerized method for the assessment of bone repair within osteoconductive porous bioceramic grafts

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