Geographical variations in fatty acid and steroid saponin biosynthesis in Dioscorea zingiberensis rhizomes

Hou, Lixiu; Li, Song; Tong, Ziyu; Yuan, Xincheng; Xu, Jiali; Li, Jiaru

doi:10.1016/j.indcrop.2021.113779

Cited by 8 publications

(10 citation statements)

References 52 publications

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“…Therefore, seedlings from Ankang of Shaanxi were used as materials to investigate the effects of JA on Dz-CYP72As and specialized metabolites. The content of dioscin in this study was lower than that in mature rhizomes, but the contents of cholesterol, stigmasterol, β-sitosterol and diosgenin were higher than that in mature rhizomes [21]. Solar energy provides the source of energy for plants and is stored via photosynthesis [36].…”

Section: Discussioncontrasting

confidence: 48%

“…According to previous research, Ankang of Shaanxi and Shiyan of Hubei are the original cities of D. zingiberensis, and the rhizomes from these two regions contained more steroidal saponins than those from other locations in China [21,35]. Therefore, seedlings from Ankang of Shaanxi were used as materials to investigate the effects of JA on Dz-CYP72As and specialized metabolites.…”

Section: Discussionmentioning

confidence: 99%

“…Phytosterols, such as cholesterol, campesterol, stigmasterol and β-sitosterol, were reported as the intermediates in the biosynthesis pathway of steroidal saponins [21]. Therefore, the contents of specialized metabolites, including phytosterols, diosgenin and steroidal saponins, were determined to investigate the impacts of JA on steroidal saponin biosynthesis.…”

Section: Effects Of Ja Concentration On the Specialized Metabolites In D Zingiberensismentioning

confidence: 99%

“…The contents of phytosterols and diosgenin were detected using the Thermo Trace gas chromatography-mass spectrometry instrument equipped with the TG-5 ms column (30 m × 0.25 mm × 0.25 µm). The operation conditions were performed as described previously [21].…”

Section: Analysis Of Specialized Metabolitesmentioning

confidence: 99%

“…The steroidal saponins were determined by an ultra-performance liquid chromatography instrument equipped with the Q Exactive hybrid quadrupole mass spectrometer (Thermo Scientific, Waltham, MA, USA) and a reverse-phase C18 column (Thermo, 100 mm × 2.1 mm, 3 µm). The mobile phases, flow rate and temperature of chromatography column were operated as described previously [21]. The gradient program was: 0-2 min, isocratic 12% B; 2-8 min, linear gradient of 12-35% B; 8-20 min, isocratic 45% B; 20-20.1 min, linear gradient of 45-20% B; 20.1-22 min, isocratic 20% B.…”

Section: Analysis Of Specialized Metabolitesmentioning

confidence: 99%

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Genome-Wide Identification of CYP72A Gene Family and Expression Patterns Related to Jasmonic Acid Treatment and Steroidal Saponin Accumulation in Dioscorea zingiberensis

Hou

Yuan

et al. 2021

IJMS

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Dioscorea zingiberensis is a medicinal herb containing a large amount of steroidal saponins, which are the major bioactive compounds and the primary storage form of diosgenin. The CYP72A gene family, belonging to cytochromes P450, exerts indispensable effects on the biosynthesis of numerous bioactive compounds. In this work, a total of 25 CYP72A genes were identified in D. zingiberensis and categorized into two groups according to the homology of protein sequences. The characteristics of their phylogenetic relationship, intron–exon organization, conserved motifs and cis-regulatory elements were performed by bioinformatics methods. The transcriptome data demonstrated that expression patterns of DzCYP72As varied by tissues. Moreover, qRT-PCR results displayed diverse expression profiles of DzCYP72As under different concentrations of jasmonic acid (JA). Likewise, eight metabolites in the biosynthesis pathway of steroidal saponins (four phytosterols, diosgenin, parvifloside, protodeltonin and dioscin) exhibited different contents under different concentrations of JA, and the content of total steroidal saponin was largest at the dose of 100 μmol/L of JA. The redundant analysis showed that 12 DzCYP72As had a strong correlation with specialized metabolites. Those genes were negatively correlated with stigmasterol and cholesterol but positively correlated with six other specialized metabolites. Among all DzCYP72As evaluated, DzCYP72A6, DzCYP72A16 and DzCYP72A17 contributed the most to the variation of specialized metabolites in the biosynthesis pathway of steroidal saponins. This study provides valuable information for further research on the biological functions related to steroidal saponin biosynthesis.

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Section: Discussioncontrasting

confidence: 48%

Section: Discussionmentioning

confidence: 99%

Section: Effects Of Ja Concentration On the Specialized Metabolites In D Zingiberensismentioning

confidence: 99%

Section: Analysis Of Specialized Metabolitesmentioning

confidence: 99%

Section: Analysis Of Specialized Metabolitesmentioning

confidence: 99%

See 3 more Smart Citations

Genome-Wide Identification of CYP72A Gene Family and Expression Patterns Related to Jasmonic Acid Treatment and Steroidal Saponin Accumulation in Dioscorea zingiberensis

Hou

Yuan

et al. 2021

IJMS

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Effect of exogenous jasmonic acid on physiology and steroidal saponin accumulation in Dioscorea zingiberensis

Hou

Song²,

Zhang³

et al. 2022

Plant Physiology and Biochemistry

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Genome-wide methylation, transcriptome and characteristic metabolites reveal the balance between diosgenin and brassinosteroids in Dioscorea zingiberensis

Li,

Geng

et al. 2024

Horticulture Research

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Diosgenin (DG) is a bioactive metabolite isolated from Dioscorea species, renowned for its medicinal properties. Brassinosteroids (BRs) are a class of crucial plant steroidal hormones. Cholesterol and campesterol are important intermediates of DG and BRs biosynthesis, respectively. DG and BRs are structurally similar components, however, the regulatory network and metabolic interplays have not been fully elucidated. In an effort to decode these complex networks, we conducted a comprehensive study integrating genome-wide methylation, transcriptome and characteristic metabolites data from Dioscorea zingiberensis. Leveraging these data, we were able to construct a comprehensive regulatory network linking DG and BRs. Mass spectrometry results enabled us to clarify the alterations in cholesterol, campesterol, diosgenin and castasterone (one of the major active BRs). The DG content decreased by 27.72% at 6 h following brassinolide treatment, whereas the content increased by 85.34% at 6 h after brassinazole treatment. Moreover, we pinpointed DG/BR-related genes, such as CASs, CYP90s and B3-ARFs, implicated in the metabolic pathways of DG and BRs. Moreover, CASs and CYP90s exhibit hypomethylation, which is closely related to their high transcription. These findings provide robust evidence for the homeostasis between DG and BRs. In conclusion, our research revealed the existence of a balance between DG and BRs in D. zingiberensis. Furthermore, our work not only provides new insights into the relationship between the two pathways but also offers a fresh perspective on the functions of secondary metabolites.

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Geographical variations in fatty acid and steroid saponin biosynthesis in Dioscorea zingiberensis rhizomes

Cited by 8 publications

References 52 publications

Genome-Wide Identification of CYP72A Gene Family and Expression Patterns Related to Jasmonic Acid Treatment and Steroidal Saponin Accumulation in Dioscorea zingiberensis

Genome-Wide Identification of CYP72A Gene Family and Expression Patterns Related to Jasmonic Acid Treatment and Steroidal Saponin Accumulation in Dioscorea zingiberensis

Effect of exogenous jasmonic acid on physiology and steroidal saponin accumulation in Dioscorea zingiberensis

Genome-wide methylation, transcriptome and characteristic metabolites reveal the balance between diosgenin and brassinosteroids in Dioscorea zingiberensis

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