Effect of exogenous jasmonic acid on physiology and steroidal saponin accumulation in Dioscorea zingiberensis

Hou, Lixiu; Song, Li; Zhang, Furui; Gu, Yongbin; Li, Jiaru

doi:10.1016/j.plaphy.2022.06.025

Cited by 2 publications

(2 citation statements)

References 36 publications

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“…As previously reported [ 26 ], the steroid profile of C. officinalis roots consisted of cholesterol, campesterol, predominant stigmasterol, sitosterol and its fully hydrogenated derivative, sitostanol, three steroidal ketones (tremulone, sitostenone, stigmastane-3,6-dione), and one derivative of sterol precursor, cycloartenol acetate. In contrast to the steroid profile of the hairy roots, neither isofucosterol nor 24-methylenecycloartanol were detected.…”

Section: Resultssupporting

confidence: 57%

“…Numerous reports on the successful application of jasmonates to enhance the production of pharmacologically important saponins, e.g., glycyrrhizin, bacoside A, madecassoside and asiaticoside, in various in vitro cultures of medicinal plants have been published in recent years [ 12 ], however, much fewer data are available on the effect of jasmonate treatment on entire plants, and some have led to ambiguous conclusions, such as in the case of the trials of enhancement of steroidal saponin accumulation in Dioscorea zingiberensis , which revealed some physiological alterations relevant to antioxidant enzymes, stomatal traits and photosynthetic efficiency [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

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The Influence of Exogenous Jasmonic Acid on the Biosynthesis of Steroids and Triterpenoids in Calendula officinalis Plants and Hairy Root Culture

Rogowska

Stpiczyńska

Pączkowski

et al. 2022

IJMS

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The interplay between steroids and triterpenoids, compounds sharing the same biosynthetic pathway but exerting distinctive functions, is an important part of the defense strategy of plants, and includes metabolic modifications triggered by stress hormones such as jasmonic acid. Two experimental models, Calendula officinalis hairy root cultures and greenhouse cultivated plants (pot plants), were applied for the investigation of the effects of exogenously applied jasmonic acid on the biosynthesis and accumulation of steroids and triterpenoids, characterized by targeted GC-MS (gas chromatography-mass spectroscopy) metabolomic profiling. Jasmonic acid elicitation strongly increased triterpenoid saponin production in hairy root cultures (up to 86-fold) and their release to the medium (up to 533-fold), whereas the effect observed in pot plants was less remarkable (two-fold enhancement of saponin biosynthesis after a single foliar application). In both models, the increase of triterpenoid biosynthesis was coupled with hampering the biomass formation and modifying the sterol content, involving stigmasterol-to-sitosterol ratio, and the proportions between ester and glycoside conjugates. The study revealed that various organs in the same plant can react differently to jasmonic acid elicitation; hairy root cultures are a useful in vitro model to track metabolic changes, and enhanced glycosylation (of both triterpenoids and sterols) seems to be important strategy in plant defense response.

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Section: Resultssupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

The Influence of Exogenous Jasmonic Acid on the Biosynthesis of Steroids and Triterpenoids in Calendula officinalis Plants and Hairy Root Culture

Rogowska

Stpiczyńska

Pączkowski

et al. 2022

IJMS

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Gibberellin Positively Regulates Tomato Resistance to Tomato Yellow Leaf Curl Virus (TYLCV)

Zhang,

Wang,

et al. 2024

Plants

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Tomato yellow leaf curl virus (TYLCV) is a prominent viral pathogen that adversely affects tomato plants. Effective strategies for mitigating the impact of TYLCV include isolating tomato plants from the whitefly, which is the vector of the virus, and utilizing transgenic lines that are resistant to the virus. In our preliminary investigations, we observed that the use of growth retardants increased the rate of TYLCV infection and intensified the damage to the tomato plants, suggesting a potential involvement of gibberellic acid (GA) in the conferring of resistance to TYLCV. In this study, we employed an infectious clone of TYLCV to inoculate tomato plants, which resulted in leaf curling and growth inhibition. Remarkably, this inoculation also led to the accumulation of GA3 and several other phytohormones. Subsequent treatment with GA3 effectively alleviated the TYLCV-induced leaf curling and growth inhibition, reduced TYLCV abundance in the leaves, enhanced the activity of antioxidant enzymes, and lowered the reactive oxygen species (ROS) levels in the leaves. Conversely, the treatment with PP333 exacerbated TYLCV-induced leaf curling and growth suppression, increased TYLCV abundance, decreased antioxidant enzyme activity, and elevated ROS levels in the leaves. The analysis of the gene expression profiles revealed that GA3 up-regulated the genes associated with disease resistance, such as WRKYs, NACs, MYBs, Cyt P450s, and ERFs, while it down-regulated the DELLA protein, a key agent in GA signaling. In contrast, PP333 induced gene expression changes that were the opposite of those caused by the GA3 treatment. These findings suggest that GA plays an essential role in the tomato’s defense response against TYLCV and acts as a positive regulator of ROS scavenging and the expression of resistance-related genes.

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Effect of exogenous jasmonic acid on physiology and steroidal saponin accumulation in Dioscorea zingiberensis

Cited by 2 publications

References 36 publications

The Influence of Exogenous Jasmonic Acid on the Biosynthesis of Steroids and Triterpenoids in Calendula officinalis Plants and Hairy Root Culture

The Influence of Exogenous Jasmonic Acid on the Biosynthesis of Steroids and Triterpenoids in Calendula officinalis Plants and Hairy Root Culture

Gibberellin Positively Regulates Tomato Resistance to Tomato Yellow Leaf Curl Virus (TYLCV)

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