Geographical Profile of<i>rpoB</i>Gene Mutations in Rifampicin Resistant<i>Mycobacterium tuberculosis</i>Isolates in Sri Lanka

Adikaram, Chamila Priyangani; Perera, Jennifer; Wijesundera, Sandhya Sulochana

doi:10.1089/mdr.2012.0031

Cited by 16 publications

(8 citation statements)

References 24 publications

(18 reference statements)

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“…Notwithstanding the fact that genotypic drug susceptibility testing has a high sensitivity and specificity but is still unable to detect all the resistance, especially in strains with novel or unknown resistance mechanisms [ 40 , 41 ]. In this review, 49 isolates with confirmed phenotypic RIF-resistance do not harbor any known mutation in the rpoB gene, which may be explained by the fact that RIF resistance-conferring mutations are present elsewhere in the rpoB gene (such as a V146F and I572F) [ 42 , 43 ], suggesting that the nature and frequency of mutations in the rpoB gene vary considerably, between different geographical regions [ 44 ], by the fact that not all the mutations are targeted by the probes used [ 45 ], or as it has been reported that molecular assays still have some drawbacks, such as product cross contamination which is a major problem leading to false positive results [ 20 ]. The reason for this cross contamination has not been elucidated properly [ 46 ], but it may be due to laboratory procedures (protocol for pretreatment, DNA extraction, and detection of the amplification product) [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, limitations of the current review included a relatively small number of studies satisfying the selection criteria, most of the selected studies had small sample sizes, and information on prior treatment status was unfortunately often lacking; we can therefore not provide reliable prevalence rates for primary or persisting infections. Several studies have shown that mutations in the rpoB gene were different from one region of the world to another TB endemic region [ 44 , 48 ]. Therefore, further studies on the characterization of drug-resistant strains are needed to describe with enough depth and clarify the behavior of the mutations found in the ropB gene analyzed in the isolates circulating in Morocco.…”

Section: Discussionmentioning

confidence: 99%

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Mutations Associated with Rifampicin Resistance in Mycobacterium tuberculosis Isolates from Moroccan Patients: Systematic Review

Eddabra

Neffa²

2020

Interdisciplinary Perspectives on Infectious Diseases

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Background. In recent years, the treatment of tuberculosis has been threatened by the increasing number of patients with drug resistance, especially rifampicin resistance, which is the most effective first-line antibiotic against Mycobacterium tuberculosis. Methods. We performed a systematic review of the literature by searching the PubMed database for studies of rifampicin-resistant Mycobacterium tuberculosis (MTB) isolates from Moroccan patients, published between 2010 and 2020. The aim of this review was to quantify the frequency of the most common mutations associated with rifampicin resistance, to describe the frequency at which these mutations co-occur. Identified studies were critically appraised according to the Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2) tool. Results. 6 studies met our inclusion criteria. Results show that 99.36% of MTB isolates had a single-point mutation, and the most commonly mutated codon of rpoB gene is 531 with 70.33% of phenotypically resistant strains. However, 10.38% of MTB strains phenotypically resistant to RIF did not exhibit any mutation in the rpoB gene. Conclusion. Identification of a resistance-associated mutation to rifampicin can be a good marker of drug-resistant TB, but lack of a mutation in the target sequence must be interpreted with caution.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Mutations Associated with Rifampicin Resistance in Mycobacterium tuberculosis Isolates from Moroccan Patients: Systematic Review

Eddabra

Neffa²

2020

Interdisciplinary Perspectives on Infectious Diseases

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“…In countries with poor epidemiological situation around TB, including former Soviet countries, strains with mutation in codon 531 (Ser531→Leu) are the most prevalent (50–70%) ones [ 6 , 7 ]. On the contrary, this codon mutation in other countries does not exceed 30–48% [ 8 – 10 ].…”

Section: Introductionmentioning

confidence: 99%

Mutations of rpoB, katG, inhA and ahp genes in rifampicin and isoniazid-resistant Mycobacterium tuberculosis in Kyrgyz Republic

Isakova¹,

Sovkhozova²,

Vinnikov

et al. 2018

BMC Microbiol

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BackgroundThe aim of this study was to identify mutations of rpoB, katG, inhA and ahp-genes associated Mycobacterium tuberculosis resistance to rifampicin (RIF) and isoniazid (INH) in Kyrgyz Republic. We studied 633 smear samples from the primary pulmonary tuberculosis (TB) patients. We verified Mycobacterium tuberculosis susceptibility to RIF and INH using culture method of absolute concentrations, and commercially available test named “TB-BIOCHIP” (Biochip-IMB, Moscow, Russian Federation).ResultsFor RIF-resistance, TB-BIOCHIP’s sensitivity and specificity were 88% and 97%, 84% and 95% for INH-resistance, and 90% and 97% for multi-drug resistance (MDR). In RIF-resistant strains, TB-BIOCHIP showed mutations in codons 531 (64.8%), 526 (17.3%), 516 (8.1%), 511 (5.4%), 533 (3.2%), 522 (0.6%) and 513 (0.6%) of rpoB gene. The most prevalent was Ser531 > Leu mutation (63.7%). 91.2% of mutations entailing resistance to INH were in katG gene, 7% in inhA gene, and 1.8% in ahpC gene. Ser315→Thr (88.6%) was the most prevalent mutation leading to resistance to INH.ConclusionsIn Kyrgyz Republic, the most prevalent mutation in RIF-resistant strains was Ser531 → Leu in rpoB gene, as opposed to Ser315 → Thr in katG gene in INH-resistant Mycobacterium tuberculosis. In Kyrgyz Republic, the major reservoir of MDR Mycobacterium tuberculosis were strains with combined mutations Ser531 → Leu in rpoB gene and Ser315 → Thr in katG gene. TB-BIOCHIP has shown moderate sensitivity with the advantage of obtaining results in only two days.

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“…But the strip can be further developed to detect other less common INH resistant mutations (AGC to AAC/AGA) in Sri Lanka by immobilizing the relevant mutation specific capture probes on to the strip, so that custom made strips can be prepared depending on the frequency and types of mutations in a particular geographical setting. Furthermore, the strip can be developed to detect MDR TB by introducing a specific probe to detect RIF resistance using most common rpoB 526 mutation (CAC to TAC) reported in Sri Lanka [31].…”

Section: Resultsmentioning

confidence: 99%

Use of nanotechnology for infectious disease diagnostics: application in drug resistant tuberculosis

et al. 2019

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Background The increased transmission of multidrug-resistant (MDR) tuberculosis (TB) poses a challenge to tuberculosis prevention and control in Sri Lanka. Isoniazid (INH) is a key element of the first line anti tuberculosis treatment regimen. Resistance to INH may lead to development of MDR TB. Therefore, early detection of INH resistance is important to curb spread of resistance. Due to the limited availability of rapid molecular methods for detection of drug resistance in Sri Lanka, this study was aimed at developing a simple and rapid gold nanoparticle (AuNP) based lateral flow strip for the simultaneous detection of the most common INH resistance mutation ( katG S315 T, 78.6%) and Mycobacterium tuberculosis (MTb). Methods Lateral flow strip was designed on an inert plastic backing layer containing a sample pad, nitrocellulose membrane and an absorption pad. Biotin labeled 4 capture probes which separately conjugated with streptavidin were immobilized on the nitrocellulose. The test sample was prepared by multiplex PCR using primers to amplify codon 315 region of the katG gene and MTb specific IS6110 region. The two detection probes complementary to the 5′ end of each amplified fragment was conjugated with gold nanoparticles (20 nm) and coupled with the above amplified PCR products were applied on the sample pad. The hybridization of the amplified target regions to the respective capture probes takes place when the sample moves towards the absorption pad. Positive hybridization is indicated by red colour lines. Results The three immobilized capture probes on the strip (for the detection of TB, katG wild type and mutation) were 100 and 96.6% specific and 100 and 92.1% sensitive respectively. Conclusion The AuNP based lateral flow assay was capable of differentiating the specific mutation and the wild type along with MTb identification within 3 h.

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Geographical Profile ofrpoBGene Mutations in Rifampicin ResistantMycobacterium tuberculosisIsolates in Sri Lanka

Cited by 16 publications

References 24 publications

Mutations Associated with Rifampicin Resistance in Mycobacterium tuberculosis Isolates from Moroccan Patients: Systematic Review

Mutations Associated with Rifampicin Resistance in Mycobacterium tuberculosis Isolates from Moroccan Patients: Systematic Review

Mutations of rpoB, katG, inhA and ahp genes in rifampicin and isoniazid-resistant Mycobacterium tuberculosis in Kyrgyz Republic

Use of nanotechnology for infectious disease diagnostics: application in drug resistant tuberculosis

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