Mutations of rpoB, katG, inhA and ahp genes in rifampicin and isoniazid-resistant Mycobacterium tuberculosis in Kyrgyz Republic

Isakova, Jainagul; Sovkhozova, Nurmira; Vinnikov, Denis; Goncharova, Z K; Talaibekova, Elnura; Aldasheva, Nazira; Aldashev, Almaz

doi:10.1186/s12866-018-1168-x

Cited by 50 publications

(54 citation statements)

References 29 publications

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“…15,16 The occurrence of 531 mutations (11.7%) in this study was comparable to 9.4% found in the previous study. 10 Our finding and those of Burkina Faso neighboring countries 11,[12][13][14] contrast enormously with the results of numerous studies [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] which reported higher rates of mutations at 531. High rates of 531 mutations were particularly found in Kyrgyzstan, Ethiopia, Brasil, South West Ethiopia, Georgia, Taiwan and Samara in Russia.…”

Section: Discussioncontrasting

confidence: 97%

Occurrence of mutations associated with rifampicin and isoniazid resistant in Mycobacterium tuberculosis isolates from patients in Burkina Faso

Diandé¹,

Ogbomon²,

Gueye³

et al. 2019

IJMBOA

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Genetic mutations are responsible for the high rate of resistance observed in the treatment of tuberculosis. This study aimed at determining the occurrence of mutations associated with rifampicin (RIF) and isoniazid (INH) resistance of Mycobacterium tuberculosis complex (MTBC) isolates. MTBC strains isolated by culture from 110 TB patients diagnosed with resistant to rifampicin (RR-TB) by Xpert MTB/RIF were studied. The isolates were obtained from the National Tuberculosis Reference Laboratory in Ouagadougou. They were identified culturally using Antigenic method (SD Bioline TB Ag MPT64). Polymerase Chain Reaction, PCR (DRplus) was used to detect the occurrence of mutations in the genes associated with resistance katG and inhA promoter for INH, and rpoB for RIF. Out of 103 isolates with RIF resistant, mutations were detected in 87(84.5%) of gene rpoB while no mutation was found in 16(15.5%) of the gene of the isolates even though the wild probes had disappeared. Single mutations were found in the codons D516V (41.7%) and H526Y (17.5%) while combined mutations (single and double) were mostly detected in the codons D516 (51.5%), H526Y (20.4%), S531L (11.7%) and H526D (10.7%) respectively. Single mutations responsible for high-level isoniazid resistance, katG were observed in the codon S315T1 while the combined inhA and katG were detected in the codon C8T and S315T, 16 (14.5%) respectively. The highest mutation occurrence was observed with rpoB516, rpoB526 for RIF and katG315 for INH associated with resistance of MTBC isolates. There is a need to improve molecular assay kit diagnosis to curb the geographic specificity of the target genes needed to detect more possible mutations.

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Section: Discussioncontrasting

confidence: 97%

Occurrence of mutations associated with rifampicin and isoniazid resistant in Mycobacterium tuberculosis isolates from patients in Burkina Faso

Diandé¹,

Ogbomon²,

Gueye³

et al. 2019

IJMBOA

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“…Of particular interest, a vast majority of isolates showed a Ser to Thr (AGC → ACC) substitution at codon 315 (63.0%). This is consistent with the results reported previously (18,19). We also detected mutations at codons 315, 431, and 439 of katG in one isolate, meanwhile, the Thr271 → Ala (ACT → GCT) substitution was detected in another isolate.…”

Section: Discussionsupporting

confidence: 93%

“…Only have one (1.1%) isolate mu-tations in both katG and inhA. This double mutation also occurred with lower frequency in our study that in studies from the Kyrgyz Republic and Kazakhstan (19,20).…”

Section: Discussioncontrasting

confidence: 51%

“…We only detected a single mutation at codon 3 in one (1.1%) isolate, codon 21 in three (3.3%) isolates, and codon 94 in one (1.1%) isolate and their amino acid substitutions were synonymous mutation (GGA → GGC), Ile21 → Thr (ATC → ACC), and Ser94 → Ala (TCG → GCG), respectively. It is worth noting that we did not detect the common frequent mutation (C-15T) in the inhA gene while this mutation was reported in phenotypically resistant isolates in many countries such as Myanmar, the Kyrgyz Republic, and Ecuador (18,19,21). It might be related to regional differences.…”

Section: Discussionmentioning

confidence: 54%

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Genotypic Characterization of katG, inhA, and ahpC in Isoniazid-Resistant Mycobacterium tuberculosis Clinical Isolates in Shanghai, China

Cao

Zhan

Guo

et al. 2020

Jundishapur J Microbiol

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Background: Mycobacterium tuberculosis is a pathogen that causes Tuberculosis and can invade various organs in infected patients. Its high morbidity and high mortality seriously threaten human health. In recent years, the continuous emergence of drug-resistant Tuberculosis bacteria has brought severe challenges to the prevention and control of Tuberculosis. Objectives: This study aimed to characterize the most frequent mutations of the katG, inhA, and ahpC genes in isoniazid (INH)resistant M. tuberculosis clinical isolates in Shanghai Pulmonary Hospital, China, and investigate the relationship between gene mutations and the minimum inhibitory concentrations (MICs) of INH against M. tuberculosis. Methods: We collected 92 INH-resistant and 30 INH-susceptible clinical isolates of M. tuberculosis. The drug resistance profiles of M. tuberculosis clinical isolates against common anti-tuberculosis drugs were determined and sequencing analysis was performed. Results: Of 92 INH-resistant strains, mutations in the katG and inhA genes were observed in 64 (69.6%) isolates and five (5.4%) isolates, respectively, and only had one (1.1%) strain both katG and inhA mutations. Among them, 62 (67.4%) strains carried a single mutation at codon 315 of the katG gene and a new mutation site was found in the katG gene of two strains. We detected a single mutation site at codon 271 and three simultaneous mutation sites at codons 315, 431, and 439. Only one (3.3%) of the 30 isoniazid-sensitive strains had the katG mutation. The AhpC mutation was detected in no experimental strains. The KatG Ser 315 Thr (AGC315ACC) mutation occurred in 53 (68.8%) out of 77 strains with high MICs (≥ 1 g/mL) of isoniazid-resistant M. tuberculosis while five (33.3%) out of 15 strains with low MICs (less than 1 g/mL) had katG Ser 315 Thr (AGC315ACC) mutation. Conclusions: Isoniazid-resistant strains were dominated by Ser315 → Thr (AGC → ACC) substitution, which seems to be associated with multidrug resistance and high-level resistance to INH. Multisite mutations are related to multidrug-resistant M. tuberculosis and the discovery of new mutation sites provides a new basis for the detection of drug-resistant M. tuberculosis.

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“…According to WHO report the average of isoniazid resistance is 7.2% in new TB cases, and 11.6% in previously treated TB cases (17). INH resistance mainly arises due to mutations in target genes katG, inhA, ahpC, kasA and ndh (18). In humans INH is readily absorbed by the gastrointestinal tract and is metabolized by N-acetyltransferases (NAT 2) present in liver and gastrointestinal tract into isonicotinic acid (INA) and acetylhydrazine (AH) (19).…”

Section: Discussionmentioning

confidence: 99%

Acetylation of isoniazid - a novel mechanism of isoniazid resistance inMycobacterium tuberculosis

Arun

Madhavan

Abraham

et al. 2020

Preprint

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Mutations of rpoB, katG, inhA and ahp genes in rifampicin and isoniazid-resistant Mycobacterium tuberculosis in Kyrgyz Republic

Cited by 50 publications

References 29 publications

Occurrence of mutations associated with rifampicin and isoniazid resistant in Mycobacterium tuberculosis isolates from patients in Burkina Faso

Occurrence of mutations associated with rifampicin and isoniazid resistant in Mycobacterium tuberculosis isolates from patients in Burkina Faso

Genotypic Characterization of katG, inhA, and ahpC in Isoniazid-Resistant Mycobacterium tuberculosis Clinical Isolates in Shanghai, China

Acetylation of isoniazid - a novel mechanism of isoniazid resistance inMycobacterium tuberculosis

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