Geo-spatial distribution of serologically detected bovine Foot and Mouth Disease (FMD) serotype outbreaks in Ilesha Baruba, Kwara State-Nigeria

Olabode, Olatunde Hamza; Kazeem, H. M.; Raji, M. A.; Ibrahim, N. D. G.; Nafarnda, Wesley Daniel

doi:10.5455/javar.2014.a20

Cited by 9 publications

(4 citation statements)

References 10 publications

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“…FMD is a serious threat for livestock population throughout the world due to its high contagiousness, rapid multiplicity of the types and sub-types, wide species of animal susceptibility, short lived immunity, and the existence of the virus as persistent or carrier state in the susceptible and carrier animals (Gurhan et al, 1993;Rodriguez et al, 1994;Saiz and Domingo, 1996;Bergmann et al, 1996;Olabode et al, 2014). The virus could be detected from vesicular fluid, oral epithelial tissue, esophageal-pharyngeal sample etc.…”

Section: Introductionmentioning

confidence: 99%

Typing of Foot and Mouth Disease Virus circulating in Bangladesh by Reverse Transcription Polymerase Chain Reaction

Hossen¹,

Ahmed²,

Khan³

et al. 2014

J. Vet. Adv.,

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Foot and Mouth Disease (FMD) is one of the highly contagious diseases of domestic and wild animals of many countries of the world including Bangladesh. The present study was undertaken to detect currently circulating FMD virus (FMDV) in Bangladesh using BHK-21 cell line, and the FMDV were typed using RT-PCR. The viruses induced characteristics cytopathic effects in BHK-21 cell lines, for examples, rounding, swelling, breaking down of intercellular bridge and cell death. Out of 151 clinical samples collected from different districts of Bangladesh, 85(56.29%) were successfully adapted in BHK-21 cell line, and were analyzed using FMDV type specific primers. Among these 85 BHK-21 adopted virus, 71(83.52%) were found as positive for FMD virus by RT-PCR; of which, 31 (36.47%) were positive for type A, 26 (30.58%) for type O, and 10 (11.76%) for type Asia 1. Only 4 (4.70%) samples were found as positive for mixed infection having Type A and Asia 1 FMDV. All the FMDV originated from Tangail (n=7) and Rajshahi (n=6) districts were found as positive for FMDV while 71.42% (n=14/36) samples of Mymensingh district were positive for FMDV. The FMDV identified in this study could now be used for effective vaccine development to control the disease in Bangladesh.

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Section: Introductionmentioning

confidence: 99%

Typing of Foot and Mouth Disease Virus circulating in Bangladesh by Reverse Transcription Polymerase Chain Reaction

Hossen¹,

Ahmed²,

Khan³

et al. 2014

J. Vet. Adv.,

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“…Seven serotypes of FMD virus such as O, A, C, SAT-1, SAT-2, SAT-3 and Asia-1 and at least 65 subtypes have been identified (Rahman and Mozumder et al, 1991;Le et al, 2012;Olabode et al, 2014). Infection with one serotype does not confer immunity against another (OIE, 2009).…”

Section: Introductionmentioning

confidence: 99%

RT-PCR based detection and adaptation of foot and mouth disease virus serotype A in BHK-21 cell line

et al. 2016

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The economic consequences of an outbreak of Foot and Mouth Disease (FMD) in Bangladesh is devastating. A sensitive, reliable and rapid diagnosis is crucial for the effective control of FMD. The present research was conducted for quick molecular detection and adaptation of FMD virus (FMDV) in BHK-21 (Baby Hamster Kidney) cell line. A two-step RT-PCR method was applied for the detection of the FMDV. Without prior adaption into BHK-21 cell culture, it is difficult to detect FMDV directly from the field samples by RT-PCR method. Samples were collected from the tongue epithelium (N=19) and inter digital tissues (N=4) of the suspected animals in Sujanagar, Bera, Santhia of Pabna district and Kotowali, Trishal of Mymensingh districts of Bangladesh during the year 2014. BHK-21 cell subculture was done from a previously cultured bottle containing BHK-21 cells. Prepared inocula were inoculated into BHK-21 cell culture and incubated at 37ºC for 24 h. After 36 h, cytopathic effects (CPE) were observed in BHK-21 cell line characterized by rounding and flattening of the cells, multinucleated giant cells formation, breaking down of the intracellular bridges and finally cell death indicated the presence of FMDV. Clear infectious BHK-21 cell culture fluid was collected and preserved at -20ºC temperature for virus detection by RT-PCR with serotype specific primers. Viral RNA was extracted from the clear infectious cell culture fluid for cDNA synthesis and used for PCR. Out of 23 samples tested, 3(13.04%) were positive for FMDV serotype A. The findings of this study can be helpful for the selection of vaccine having specific FMDV type, and it may help in controlling FMD in Bangladesh.Progressive Agriculture 27 (1): 64-69, 2016

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“…Thus suggesting increasing FMD virus cross infections between cloven hoofed livestock animals as well as environmental contamination especially within and between the North-Central vegetation zones of the country. These potential FMD circulating serotypes and their geo-spatial distribution in pigs within these ecological zones require further clarification as previously conducted by Olabode et al, (2014c). Questionnaire findings indicates pig farmers in this study area, mostly engage in mono-livestock keeping with adequate biosecurity practice and good husbandry system as this enhances their ability to know and identify FMD especially in younger pigs.…”

Section: Table 1 Demographic Distribution Of Swine Fmd Antibodies Pre...mentioning

confidence: 60%

Serological detection of foot and mouth disease antibodies in apparenty healthy pigs within federal capital territory, Abuja

EchiodaOgbole,

Olabode,

Maliafia

et al. 2023

Nig. Vet. J.

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Swine production contributes significantly to the economic wealth of most farmers in developing nations including Nigeria. Foot-and-mouth disease (FMD) has been a highly contagious viral disease of agricultural importance in domestic ruminants and pigs, hampering livestock productivity in countries where the disease is still endemic. The study aimed to determine the swine FMD occurrence in order to elucidate the disease burden within Federal Capital Territory, Abuja and its environs. One hundred (100) sera were collected from Pigs and subjected to 3ABC ELISA test at the FMD Laboratory in National Veterinary Research Institute (NVRI), Vom. The result showed that out of 100 sera samples tested for FMD antibodies, 26 (26%) were positive and 74 (74%) were negative. Sero-positive distribution showed sow/gilt (32.7%), younger pigs (57.7%) and local breeds (50%) were more exposed to FMD. Although, this was not significant (P>0.05). Questionnaire report further showed knowledge attitude and practice (KAP) of farmers was good, however, the absence of vaccination could be a contributing factor to FMD spread to other susceptible and between carrier livestock within the study area. This finding suggests an increasing occurrence of FMD in pigs within the Guinea Savanna Zone of North-Central Nigeria. In conclusion, FMD antibodies were detected in apparently healthy, unvaccinated pigs of all breeds, ages and sex slaughtered and reared in the FCT. Control efforts should be put in place to prevent possible transmission of Foot and Mouth Disease between pigs and cattle and other ruminants within and between herds, market and slaughter slabs.

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Geo-spatial distribution of serologically detected bovine Foot and Mouth Disease (FMD) serotype outbreaks in Ilesha Baruba, Kwara State-Nigeria

Cited by 9 publications

References 10 publications

Typing of Foot and Mouth Disease Virus circulating in Bangladesh by Reverse Transcription Polymerase Chain Reaction

Typing of Foot and Mouth Disease Virus circulating in Bangladesh by Reverse Transcription Polymerase Chain Reaction

RT-PCR based detection and adaptation of foot and mouth disease virus serotype A in BHK-21 cell line

Serological detection of foot and mouth disease antibodies in apparenty healthy pigs within federal capital territory, Abuja

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Geo-spatial distribution of serologically detected bovine Foot and Mouth Disease (FMD) serotype outbreaks in Ilesha Baruba, Kwara State-Nigeria

Cited by 9 publications

References 10 publications

Typing of Foot and Mouth Disease Virus circulating in Bangladesh by Reverse Transcription Polymerase Chain Reaction

Typing of Foot and Mouth Disease Virus circulating in Bangladesh by Reverse Transcription Polymerase Chain Reaction

RT-PCR based detection and adaptation of foot and mouth disease virus serotype A in BHK-21 cell line

Serological detection of foot and mouth disease antibodies in apparenty healthy pigs within federal capital territory, Abuja

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RT-PCR based detection and adaptation of foot and mouth disease virus serotype A in BHK-21 cell line