Genus-Specific Detection of Salmonellae using the Polymerase Chain Reaction (PCR)

Cohen, Noah D.; Neibergs, Holly L.; McGruder, Edward D.; Whitford, H. W.; Behle, Robert W.; Ray, P. M.; Hargis, Billy M.

doi:10.1177/104063879300500311

Cited by 87 publications

(68 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Upper strand and Lower strand primer was used. 19 In the present study, it was found that the E. coli isolated from broiler were sensitive to ciprofloxacin and gentamicin. The results strengthen the earlier observations of some study.…”

Section: Discussionmentioning

confidence: 79%

“…[15][16][17] Isolates of E. coli observed in EMB agar revealed smooth, circular, greenish black color colonies with metallic sheen and pink color colonies on McConkey agar. [18][19][20][21] In Gram's staining, the morphology of the isolated bacteria exhibited Gram-negative, short rod arranged in single or paired. 22,23 The Salmonella revealed smooth, circular, black centered colonies on XLD agar.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation, identification, and antibiogram studies of Salmonella species and Escherichia coli from boiler meat in some selected areas of Bangladesh

Al-Salauddin

Hossain

Dutta

et al. 2015

Int J Basic Clin Pharmacol

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Isolation, identification, and antibiogram studies of Salmonella species and Escherichia coli from boiler meat in some selected areas of Bangladesh

Al-Salauddin

Hossain

Dutta

et al. 2015

Int J Basic Clin Pharmacol

View full text Add to dashboard Cite

“…1,2,4,21 The invA gene is the target of many of these methods because it not only is specific to the Salmonella genus, but it is also found in all known serovars of Salmonella.…”

Section: Introductionmentioning

confidence: 99%

Use of Pooled Samples for the Detection of Salmonella in Feces by Polymerase Chain Reaction

et al. 2006

View full text Add to dashboard Cite

Abstract. Many epidemiological studies of Salmonella rely on conventional bacteriological culture methods to detect Salmonella in fecal samples. These culture-based methods are inefficient for epidemiological studies in populations with a low prevalence of Salmonella. The objective of this study was to optimize a protocol that uses pooled Salmonella enrichment broth cultures of bovine feces and polymerase chain reaction (PCR) for the detection of the invA gene of Salmonella in feces. In one field trial, 196 animals were sampled, and all samples were tested by culture, invA PCR on individual samples, invA PCR on pools of 5 samples, and BAX PCR on individual samples. All assays showed a high agreement on individual samples (kappa $ 0.75). The invA PCR was run on each of 40 pools and detected 19 of 22 culture-positive pools. In another field trial, 152 samples were taken from 4 dairies, and the invA PCR was performed on pools of 5 samples in addition to bacteriological culture of individual samples. Salmonella was detected in 5 of the 32 pools (7 total positive samples) by both PCR and culture. One pool was PCR-positive but culture-negative. Pooling did not dramatically affect the performance of the invA PCR; most of the culture-positive samples were detected, including all of the samples when there were 4 or more Salmonella colonies on the agar plate. Based on these field trials, invA PCR on pooled samples appears to be an efficient method of Salmonella detection as long as Salmonella loads are not extremely low.

show abstract

“…The Histidine transport operon and JEO402-1 genes are reported as highly conserved regions in all Salmonella species [12,13]. The presence of these two genes in our study was distinctive of all four strains of Salmonella evaluated.…”

Section: Discussionmentioning

confidence: 55%

“…Primers with highly conserved regions to detect pathogenic Salmonella were selected from the literature: invA [10], fimC [11], JE0402-1 [12], and histidine operon transport [13]. The cocktail for the PCR reaction contained 4 µl of genomic DNA (100 ng/µl) obtained by heat shock treatment and 16 µl of the following solution: 5% Glycerol, 1.5 Mm MgCl2, 0.8 Mm dNTPs, 0.2 µM of each primer and 0, 05 U de Platinum Taq-polymerase (Invitrogen, United States).…”

Section: Pcr Detectionmentioning

confidence: 99%

Isolation of Lytic Bacteriophages for Nanobiocontrol of Pathogenic and Antibiotic Resistant Salmonella Present in Poultry in Ecuador

Quiroz¹,

Recalde²,

Arias³

et al. 2016

Biol Med (Aligarh)

View full text Add to dashboard Cite

The genus Salmonella, as many others bacteria, has been frequently reported as resistant to commonly used antibiotics in poultry, therefore, the current spread of antibiotic resistance genes in bacteria, raises doubts on the effectiveness of antibiotics in the future. One possible alternative to antibiotics is the use of bacteriophages as antimicrobial agents to control antibiotic resistant pathogenic bacteria. The main goal of this project was to isolate and use bacteriophages as a new, safe, and effective biocontrol method for treating infectious diseases caused by Salmonella entero-pathogena. Four lytic bacteriophages cocktails (PSEA-2, SSEA, PSIA-2, SSIA) were isolated from wastewater of poultry processing industries, to control Salmonella enterica subsp entérica serovar Enteritidis (SE), and Salmonella entérica subsp enterica serovar Infantis (SI), under laboratory conditions. We found the cocktails PSEA-2 and PSIA-2 specific for SE and SI while cocktails SSEA and SSIA also caused lysis in Pseudomona. The observations in the Transmission Electron Microscope (TEM) revealed the presence of tailed phages of the Siphovididae and Myoviridae families; and a polyhedral phage. We have isolated specific phages and tested in vitro their effectiveness at controlling Salmonella. Further studies are needed in order to assess the phage effectiveness in vivo.

show abstract

Genus-Specific Detection of Salmonellae using the Polymerase Chain Reaction (PCR)

Cited by 87 publications

References 9 publications

Isolation, identification, and antibiogram studies of Salmonella species and Escherichia coli from boiler meat in some selected areas of Bangladesh

Isolation, identification, and antibiogram studies of Salmonella species and Escherichia coli from boiler meat in some selected areas of Bangladesh

Use of Pooled Samples for the Detection of Salmonella in Feces by Polymerase Chain Reaction

Isolation of Lytic Bacteriophages for Nanobiocontrol of Pathogenic and Antibiotic Resistant Salmonella Present in Poultry in Ecuador

Contact Info

Product

Resources

About