Gentiopicroside prevents interleukin-1 beta induced inflammation response in rat articular chondrocyte

Zhao, Lei; Ye, Juan; Guotai, Wu; Peng, Xuejing; Xia, Peng-Fei; Ren, Yuan

doi:10.1016/j.jep.2015.06.031

Cited by 74 publications

(51 citation statements)

References 51 publications

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“…The result showed that loganic acid, swertiamarin, gentiopicroside and sweroside are the major chemical constituents in GME. This result is in accord with previous report …”

Section: Resultssupporting

confidence: 94%

“…In recent years, more and more evidences have indicated that gentiopicroside and swertiamarin are the major pharmacological constituents for anti‐inflammation and antinociceptive. These two compounds have been used as the marker ingredients in this plant . Modern pharmacological study showed that ethanol or water extract of G. macrophylla root can be used for protective agent for liver damage.…”

Section: Introductionmentioning

confidence: 99%

“…These two compounds have been used as the marker ingredients in this plant. [6][7][8][9] Modern pharmacological study showed that ethanol or water extract of G. macrophylla root can be used for protective agent for liver damage. Kang et al [10] reported that the water extract of G. macrophylla root showed protective effect on acute liver injury induced by CCl 4 in mice through reducing oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

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Anti-alcohol liver disease effect ofGentianae macrophyllaeextract through MAPK/JNK/p38 pathway

Cui

Jiang

Shao

et al. 2018

Journal of Pharmacy and Pharmacology

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Objectives The hepatoprotective effect of Gentianae macrophyllae root extract (GME) on alcoholic liver disease (ALD) was evaluated through ethanol induced ALD animal model. Methods Mice were randomly divided into control normal group (10 mice), ethanol‐induced ALD model group (10 mice) and GME plus ethanol group (30 mice). Mice in model group were given intragastric administration with 50% (v/v) ethanol aqueous solution (200 μl for each) once daily for 19 days. Mice in control normal group received equal volumes of water. Mice in GME plus ethanol group were given intragastric administration with 50% (v/v) ethanol aqueous solution (200 μl for each) once daily at 10:00 a.m., after 1 h, mice in GME group sequentially were treated with 20, 40 and 100 mg/kg of GME by gastric gavage for 19 days. the average food and water consumed by the mice in every group were recorded every 2 days and body weight of every mouse in every group was measured every 2 days. Key findings Results showed that GME significantly improved alcohol induced liver injury in a dose‐dependent manner. The impaired hepatic tissue structure was repaired and the collagen deposition declined after GME administration. Meanwhile, the level of malonaldehyde (MDA), Aspartate transaminase (AST) and alanine transaminase (ALT) (indicators of liver damage) in blood serum were significantly controlled by GME with a dose‐dependent manner, moreover, body weight and liver index were also improved after administration of GME. Pro‐inflammatory cytokines including MCP‐1, TNF‐α, IL‐1 and IL‐6 were detected through RT‐PCR and ELISA in experiment and GME can significantly inhibit the expression of TNF‐α, IL‐1 and IL‐6 but have no effect on MCP‐1. In order to explore the mechanism of GME on ALD, MAPKs pathway was examined and results indicated that GME attenuated ALD through inhibiting the phosphorylation of JNK and P38 and further suppressing the initiation of inflammation. Conclusions GME attenuated ALD through inhibiting the phosphorylation of JNK and P38 and further suppressing the initiation of inflammation.

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“…The result showed that loganic acid, swertiamarin, gentiopicroside and sweroside are the major chemical constituents in GME. This result is in accord with previous report …”

Section: Resultssupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Anti-alcohol liver disease effect ofGentianae macrophyllaeextract through MAPK/JNK/p38 pathway

Cui

Jiang

Shao

et al. 2018

Journal of Pharmacy and Pharmacology

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“…Several lines of evidence indicate that Veratric acid [33], Loganic acid [34], Paeoniflorin [35], Loganin [36] and apiin [37] have anti-inflammatory and immune-regulatory activities. Gentiopicroside [38], Astragaloside IV [39], Acteoside [40] and Prime-O-glucosylcimifugin [41] exert anti-inflammatory effects by inhibiting the activation of NF-κB and other signaling pathways. Isoimperatorin can inhibit TNFα-induced expression of VCAM-1 by up-regulating the production of PPAR-γ and translating signals to ERK1/2, PI3K, and PKC [42].…”

Section: Discussionmentioning

confidence: 99%

Herbal formula Xian-Fang-Huo-Ming-Yin regulates differentiation of lymphocytes and production of pro-inflammatory cytokines in collagen-induced arthritis mice

Li¹,

Wei²,

Li³

et al. 2017

BMC Complement Altern Med

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BackgroundXian-Fang-Huo-Ming-Yin (XFHM), a traditional herbal formula, has been used to treat sores and carbuncles for hundreds of years in Asia. Nowadays, its clinical effects in treatment of rheumatoid arthritis (RA) have been validated. In this study, we want to study its possible molecular mechanisms of regulating the differentiation of lymphocytes and production of pro-inflammatory cytokines in collagen-induced arthritis (CIA) mice for RA treatment.MethodsA high performance liquid chromatography-electrospray ionization/mass spectrometer (HPLC-ESI/MSn) system was used to analyze the constituents of XFHM granules. An arthritics mouse model was induced by collagen and leflunomide (LEF) was used as a positive control medicine. Pathological changes at the metatarsophalangeal joint were studied through Safranin O and immunohistochemical staining. The differentiation of T, B and NK cells was examined by flow cytometry and pro-inflammatory cytokines were assayed using an Inflammation Antibody Array assay. The expression of key molecules of the nuclear factor κB (NF-κB) and Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathways in spleen were studied by western-blot analysis.ResultsIn our study. 21 different dominant chemical constituents were identified in XFHM. Treatment with XFHM suppressed the pathological changes in arthrosis of CIA. Additionally, XFHM down-regulated the proliferation and differentiation of CD3+ T cells and CD3−CD19+ B cells significantly. However, XFHM had no significant effect on CD3−NK1.1+ NK cells. Further study showed that the production of pro-inflammatory cytokines had been suppressed by inhibiting the activation of NF-κB and JAK/STAT signaling.ConclusionsXFHM can regulate and maintain the immunologic balance of lymphocytic immunity and inhibit the production of pro-inflammatory cytokines, thus suppressing the pathological changes of RA. Therefore, XFHM may be used as an application of traditional medicine against RA in modern complementary and alternative therapeutics.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1526-x) contains supplementary material, which is available to authorized users.

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“…Meanwhile many studies indicated that gentiopicroside exhibited excellent anti-inflammatory activity. One study showed that gentiopicroside has a potent protective effect on IL-1β-induced inflammation, which may be related to inhibiting the release of inflammatory mediators and NF-κB p65 protein expression [45,46]. Another study showed that gentiopicroside reduced the levels of IL-1β, IL-6, and TNF-α in serum as well as down-regulated the levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2).…”

Section: Discussionmentioning

confidence: 99%

Antidermatophyte Activity of the Gentiopicroside-Rich n-Butanol Fraction from <b><i>Gentiana siphonantha</i></b> Maxim. Root on a Guinea Pig Model of Dermatophytosis

et al. 2018

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Background:Gentiana siphonantha Maxim. is a traditional medicine for the treatment of rheumarthritis, icterepatitis, pain and hypertension; it is rich in gentiopicroside with anti-inflammatory, antibacterial, and free radical-scavenging activities. This study was to evaluate the antidermatophyte activity of G. siphonantha on a guinea pig model in vitro and in vivo. Material andMethods: The antidermatophyte activities of 10 plants were tested by the broth microdilution method. Fractions and an extract of G. siphonantha were tested against Trichophyton mentagrophytes by the disc diffusion method. The morphological changes of T. mentagrophytes were observed. Component analysis of the n-butanol (n-BuOH) fraction was made by HPLC. Finally, the antifungal activity in an in vivo guinea pig model of dermatophytosis was examined. Results:G. siphonantha had strong antidermatophyte activity with MIC₅₀ values of 32-64 μg/mL. The n-BuOH fraction of G. siphonantha showed the most potent activity compared to the other fractions. After being exposed to the n-BuOH fraction at 80 and 160 μg/mL, the hyphae were distorted and collapsed. Gentiopicroside is the main active ingredient in the n-BuOH fraction of G. siphonantha. The lesion scores of the guinea pig model of dermatophytosis significantly declined in the 10% and 30% extract and positive control groups in comparison with the untreated control group. Periodic acid-Schiff and hematoxylin/eosin staining displayed similar results. Conclusion: The n-BuOH fraction of G. siphonantha demonstrated antidermatophyte efficacy in experimental dermatophytosis.

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Gentiopicroside prevents interleukin-1 beta induced inflammation response in rat articular chondrocyte

Cited by 74 publications

References 51 publications

Anti-alcohol liver disease effect ofGentianae macrophyllaeextract through MAPK/JNK/p38 pathway

Anti-alcohol liver disease effect ofGentianae macrophyllaeextract through MAPK/JNK/p38 pathway

Herbal formula Xian-Fang-Huo-Ming-Yin regulates differentiation of lymphocytes and production of pro-inflammatory cytokines in collagen-induced arthritis mice

Antidermatophyte Activity of the Gentiopicroside-Rich n-Butanol Fraction from <b><i>Gentiana siphonantha</i></b> Maxim. Root on a Guinea Pig Model of Dermatophytosis

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