Genotyping with RAPD and microsatellite markers resolves pathotype diversity in the ascochyta blight pathogen of chickpea

Genetic diversity and population structure of Ascochyta rabiei from the western Iranian Ilam and Kermanshah provinces using MAT and SSR markers

“…Molecular markers have been widely deployed to detect and identify A. rabiei isolates and to examine genetic diversity, genetic structure, and virulence in populations of this fungus (Morjane et al 1994;Peever et al 2004;Phan et al 2003;Santra et al 2001;Udupa et al 1998). Significant genetic variation was shown in populations of A. rabiei in Italy (Fischer et al 1995), Tunisia Morjane et al 1994), Syria and Lebanon (Udupa et al 1998), Spain (NavasCortés et al 1998), Pakistan (Jamil et al 2000) and India (Santra et al 2001). In some regions of the world where chickpea has only recently been introduced, for example Australia and California, only modest genetic diversity and a single mating type has been reported (Kaiser 1997;Phan et al 2003).…”

An Update on Genetic Resistance of Chickpea to Ascochyta Blight

“…In A. rabiei of chickpea, a number of pathotypes were reported; for instance, more than ten pathotypes by Vir and Grewal, [32]; five pathotypes by Nene and Reddy, [1] and three pathotypes by Udupa et al [33]. Udupa et al [33] reported the occurrence of three pathotypes; pathotype I (less aggressive), pathotype II (aggressive) and pathotype III (most aggressive) as revealed by molecular markers [26,[34][35][36]. A new A. rabiei pathotype (pathotype IV) was reported in Syria that is capable of affecting the highly resistant chickpea genotypes (ICC-12004 and ICC-3996) known for their resistance to pathotypes I, II and III.…”

“…The presence of a teleomorph (D. rabiei) in the A. rabiei life cycle contributes to variability within the pathogen population, which may generate a new combination of virulence genes and the development of new pathotypes [11]. In A. rabiei of chickpea, a number of pathotypes were reported; for instance, more than ten pathotypes by Vir and Grewal, [32]; five pathotypes by Nene and Reddy, [1] and three pathotypes by Udupa et al [33]. Udupa et al [33] reported the occurrence of three pathotypes; pathotype I (less aggressive), pathotype II (aggressive) and pathotype III (most aggressive) as revealed by molecular markers [26,[34][35][36].…”

Genetic and Pathogenic Variability of Ascochyta rabiei Isolates from Pakistan and Syria as Detected by Universal Rice Primers

“…Pathotype variability is necessary to select the appropriate pathotype for screening genotypes in resistance breeding programme [2]. Differentiation of Ascochyta rabiei (AR) into 3 classes (pathotype I, II and III) was reported in Syria and has been widely accepted [3] and recently highly aggressive pathotype IV has been reported by Imtiaz et al [4]. In Pakistan three pathotypes were also identified by Jamil et al [5] and Ali et al [6] using chickpea differential genotypes (ILC1929, ILC482 and ILC3279) and (Spanish white, Dwelley and ICC12004) respectively.…”

“…The isolates were also characterized using 3 SSR markers [8] and 10 Universal rice primers (URP) that are repeat motifs obtained from Korean weedy rice and have been utilized in diverse genome like animals, plants and microbes [9]. Previous studies were based on RAPD and SSR markers for the assessment of genetic diversity of AR isolates [3,5,6,[10][11][12][13]. Only SSR markers were reported by Imtiaz et al (2011) to differentiate pathotype III and IV from Syria.…”