Genotyping of Novel SNPs in BMPR1B, BMP15, and GDF9 Genes for Association with Prolificacy in Seven Indian Goat Breeds

Ahlawat, Sonika; Sharma, Rekha; Roy, Manoranjan; Mandakmale, S. D.; Prakash, Ved; Tantia, M. S.

doi:10.1080/10495398.2016.1167706

Cited by 37 publications

(47 citation statements)

References 35 publications

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“…The A959C and G1189A were genotyped by Ahlawat et al. () using RFLP‐PCR and by Ahlawat et al. () and Maitra, Sharma, Ahlawat, Borana, and Tantia () using T‐ARMS‐PCR in Indian goats.…”

Section: Discussionmentioning

confidence: 99%

“…Ahlawat et al. () found a T(‐242)C in BMPR1B gene of seven Indian goat breeds. In another study, Ahlawat et al.…”

Section: Discussionmentioning

confidence: 99%

“…Ahlawat et al. () genotyped novel SNPs in BMPR1B, BMP15 and GDF9 genes and investigated their association with litter size in seven Indian goat breeds. The results showed that none of SNPs (including G1189A and A959C in GDF9 and T(‐242)C in BMPR1B) were associated with litter size.…”

Section: Discussionmentioning

confidence: 99%

“…Two SNPs in the promoter region of BMPR1B gene (Ahlawat, Sharma, Maitra, Roy, & Tantia, ) and three non‐synonymous SNPs in exon 2 of GDF9 gene (Ahlawat, Sharma, Roy, Tantia, & Prakash, ) in Black Bengal goat were reported. Novel genetic variations in GDF9 and BMPR1B genes did not contribute to the reproductive ability of seven Indian goat breeds (Ahlawat et al., ). In this study, we hypothesized that there would be polymorphisms in the GDF9 and BMPR1B genes of Markhoz goat that would associate with litter size.…”

Section: Introductionmentioning

confidence: 99%

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Polymorphism of GDF9 and BMPR1B genes and their association with litter size in Markhoz goats

Shokrollahi

Morammazi

2018

Reprod Domestic Animals

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The main objective of this study was to investigate the polymorphism of GDF9 and BMPR1B genes and their relationship with litter size in Markhoz goats. The polymorphism of GDF9 and BMPR1B genes as well-documented genes regarding fecundity in sheep and goat was investigated using RFLP-PCR and a tetra-primer amplification refractory mutation system-PCR (T-ARMS-PCR) in Markhoz goats. The 164 blood samples were collected from the raised goats in Sanandaj Markhoz goat Performance Testing Station. The DNA extraction was carried out by salting-out procedure, and then, PCR was performed using four and two pairs of primers to detect polymorphism in GDF9 and BMPR1B genes, respectively. To disclose GDF9 loci polymorphism, PCR products were digested with SspI (G3288A), PvuII (G423A), MvaI (A959C) and MspI (G1189A) restriction enzymes. The results showed that these mutations are available in tested animals. Parity had no significant effect on litter size. Also, the effects of different genotypes of GDF9 and BMPR1B had no significant effect on litter size. Further studies with a high number of animals with minimum relatedness for testing the association of these SNPs and others in the fecundity genes with reproductive traits may be worthwhile.

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“…The A959C and G1189A were genotyped by Ahlawat et al. () using RFLP‐PCR and by Ahlawat et al. () and Maitra, Sharma, Ahlawat, Borana, and Tantia () using T‐ARMS‐PCR in Indian goats.…”

Section: Discussionmentioning

confidence: 99%

“…Ahlawat et al. () found a T(‐242)C in BMPR1B gene of seven Indian goat breeds. In another study, Ahlawat et al.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Polymorphism of GDF9 and BMPR1B genes and their association with litter size in Markhoz goats

Shokrollahi

Morammazi

2018

Reprod Domestic Animals

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“…[31] reported that the FecB gene had no significant effect on prolificacy of Jining Gray goats whereas Ahlawat et al . [32] have shown that FecB , BMP15, and GDF9 genes have no effect on prolificacy of seven Indian goat breeds including the highly prolific Black Bengal goats. Findings of Ghaffari et al .…”

Section: Resultsmentioning

confidence: 99%

Allelic and genotypic frequencies in polymorphic Booroola fecundity gene and their association with multiple birth and postnatal growth in Chhotanagpuri sheep

Oraon¹,

Singh²,

Ghosh³

et al. 2016

Vet World

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Aim:Chhotanagpuri breed of sheep reared for mutton in Jharkhand, India, having problem of low litter size and body weight. The response of genetic improvement for traits with low heritability through traditional selection method is time-consuming. Therefore, marker-assisted selection based on a polymorphism study of suitable candidate gene can response quickly. Thus, this study was aimed at identification of different allelic and genotypic frequencies of Booroola fecundity (FecB) gene and its association with multiple birth and postnatal growth in Chhotanagpuri sheep.Materials and Methods:DNA isolation and gene-specific amplification of FecB gene was performed from blood samples of from 92 Chhotanagpuri lambs maintained under similar feeding and management conditions. Custom nucleotide sequencing and single-strand conformational polymorphism analysis were performed to identify different genotypes with respect to the target gene. Statistical analysis was performed for determination of allelic and genotypic frequencies of FecB gene polymorphisms and its association with multiple birth and postnatal growth of lambs from birth to 52 weeks age.Results:“AA,” “AB,” and “BB” genotypes were found at locus-1 as it is polymorphic for FecB gene while locus-2 was found to be monomorphic for FecB gene. Higher frequency of “A” allele at locus-1 was found in single born lambs, whereas “B” allele was predominant among multiple born lambs. The lambs having “BB” genotype weighed significantly (p≤0.01) heavier than those of “AB” and “AA” genotype at 52 weeks of age.Conclusion:“BB” genotype has emerged as favored genotype for multiple births and better growth indicator. Therefore, homozygous lambs for “B” allele should be selected and utilized in breeding program for better growth rate.

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Comparison of the sequences and expression levels of genes related to follicular development and atresia between prolific and nonprolific goat breeds

Lü

et al. 2019

Veterinary Medicine & Sci

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This study investigated the variations of the nucleotide sequences and ovarian expression levels of genes related to follicular development and atresia in prolific Jintang black goats and nonprolific Tibetan goats. Eight genes, FSHB, LHB, FSHR, LHCGR, ESR2, B4GANT2, BCL2 and BAX, were examined using reverse transcription‐polymerase chain reaction and quantitative real‐time PCR. The results showed that the nucleotide and deduced amino acid sequences of the LHB and BAX genes were not different, but there was one base change in the FSHR genes between the two breeds. There was one base change in the FSHB gene, which resulted in one amino acid substitution; there were nine base changes in the LHCGR gene, which resulted in five amino acid substitutions; and there were six base changes in the B4GANT2 gene, which resulted in four amino acid substitutions. The expression levels of the FSHR, LHCGR, ESR2, B4GANT2, BCL2 and BAX genes in the ovaries were not different between the two breeds. The plasma concentrations of FSH were not different, but the plasma concentrations of LH, P4 and E2 were lower in prolific Jintang black goats than in nonprolific Tibetan goats (P ˂ 0.05) at 40 hr after removal of the Controlled Internal Drug Release Devices. These results provide some foundations elucidating the endocrine and molecular mechanisms controlling ovulation rate in goats, but these need to be further verified.

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Genotyping of Novel SNPs in BMPR1B, BMP15, and GDF9 Genes for Association with Prolificacy in Seven Indian Goat Breeds

Cited by 37 publications

References 35 publications

Polymorphism of GDF9 and BMPR1B genes and their association with litter size in Markhoz goats

Polymorphism of GDF9 and BMPR1B genes and their association with litter size in Markhoz goats

Allelic and genotypic frequencies in polymorphic Booroola fecundity gene and their association with multiple birth and postnatal growth in Chhotanagpuri sheep

Comparison of the sequences and expression levels of genes related to follicular development and atresia between prolific and nonprolific goat breeds

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