Comparison of the sequences and expression levels of genes related to follicular development and atresia between prolific and nonprolific goat breeds

Zi, Xiang-Dong; Hu, Liang; Lü, Jing; Liu, Shuang; Zheng, Yusheng

doi:10.1002/vms3.225

Cited by 7 publications

(7 citation statements)

References 55 publications

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“…In this case, the overexpression of B4GALNT2 in the ovary leads to atypical glycosylation of inhibin (inhibin is an important hormone that regulates ovarian function), which promotes high fecundity in sheep 54 . In addition, previous studies have shown that this gene is related to follicular development and atresia 55 . EEPD1 maintains the genome stability during embryonic replication stress by promoting homologous recombination (HR) and inhibiting non-homologous end joining (NHEJ) 56 .…”

Section: Resultsmentioning

confidence: 92%

Analysis on the desert adaptability of indigenous sheep in the southern edge of Taklimakan Desert

Chenglong

Liu

Zhang

et al. 2022

Sci Rep

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The southern margin of the Taklimakan Desert is characterized by low rainfall, heavy sandstorms, sparse vegetation and harsh ecological environment. The indigenous sheep in this area are rich in resources, with the advantages of perennial estrus and good resistance to stress in most sheep. Exploring the molecular markers of livestock adaptability in this environment will provide the molecular basis for breeding research to cope with extreme future changes in the desert environment. In this study, we analyzed the population genetic structure and linkage imbalance of five sheep breeds with three different agricultural geographic characteristics using four complementary genomic selection signals: fixation index (FST), cross-population extended haplotype homozygosity (xp-EHH), Rsb (extended haplotype homozygosity between-populations) and iHS (integrated haplotype homozygosity score). We used Illumina Ovine SNP 50K Genotyping BeadChip Array, and gene annotation and enrichment analysis were performed on selected regions of the obtained genome. The ovary of Qira Black sheep (Follicular phase, Luteal phase, 30th day of pregnancy, 45th day of pregnancy) was collected, and the differentially expressed genes were screened by transcriptomic sequencing. Genome-wide selective sweep results and transcriptome data were combined for association analysis to obtain candidate genes associated with perennial estrus and stable reproduction. In order to verify the significance of the results, 15 resulting genes were randomly selected for fluorescence quantitative analysis. The results showed that Dolang sheep and Qira Black sheep evolved from Kazak sheep. Linkage disequilibrium analysis showed that the decay rate of sheep breeds in the Taklimakan Desert was higher than that in Yili grassland. The signals of FST, xp-EHH, Rsb and iHS detected 526, 332, 308 and 408 genes, respectively, under the threshold of 1% and 17 overlapping genes under the threshold of 5%. A total of 29 genes were detected in association analysis of whole-genome and transcriptome data. This study reveals the genetic mechanism of perennial estrus and environmental adaptability of indigenous sheep breeds in the Taklimakan Desert. It provides a theoretical basis for the conservation and exploitation of genetic resources of indigenous sheep breeds in extreme desert environment. This provides a new perspective for the quick adaptation of sheep and other mammals to extreme environments and future climate changes.

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Section: Resultsmentioning

confidence: 92%

Analysis on the desert adaptability of indigenous sheep in the southern edge of Taklimakan Desert

Chenglong

Liu

Zhang

et al. 2022

Sci Rep

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“…follicular development, egg maturation, and hormonal regulation of endometrial development. It has been shown that the expression of genes responsible for follicular development and atresia differs in different goat breeds, and thus is genetically determined [21]. However, the results of other groups of scientists also highlight the influence of environmental conditions, such as husbandry, climate, and feeding, on the pregnancy rate [10].…”

Section: Resultsmentioning

confidence: 99%

Reproductive characteristics of Saanen and Alpine bucks

Bogdaniuk,

Garkavii,

Petrushko

2023

Bìol. Tvarin

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Genetic variations among breeds within a species can impact not only productivity traits, such as milk yield and quality, but also animal health, including fertility. This study aimed to compare the reproductive characteristics of bucks from the Saanen and Alpine breeds. Sperm concentration and motility were assessed using light microscopy, viability was determined using eosin-nigrosin staining, and morphological parameters were evaluated using the Spermac Stain method. DNA fragmentation was measured using the Halosperm kit. Artificial insemination of goats was conducted with fresh semen during natural estrus. Statistical analysis was performed using the Graph Pad Prism software. The results revealed that Alpine bucks exhibited significantly higher semen volume, sperm concentration, viability, and motility (P<0.05). No significant differences (P≥0,05) were observed between the breeds regarding the number of spermatozoa with normal morphology and the rate of DNA fragmentation. Cryobiological analysis of spermatozoa from Saanen bucks suggested a higher cryoresistance compared to the Alpine breed. Following artificial insemination of goats, the pregnancy rate for the Saanen breed was 61.8%, which was twice as high as that observed in Alpine goats — 28.8% (P<0.05). These findings demonstrate significant differences in reproductive characteristics between Saanen and Alpine goats. Despite superior sperm characteristics, the pregnancy rate after artificial insemination was significantly lower in the Alpine breed compared to the Saanen breed. Consequently, it is crucial to consider these variations in essential reproductive characteristics when implementing breeding programs and employing reproductive biotechnology in animal husbandry to ensure their successful application and effectiveness.

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“…Growth factors play an important role in the development and maintenance of follicles. Bone morphogenetic protein receptor 1B (BMPR-1B), bone morphogenetic protein 15 (BMP15), growth differentiation factor 9 (GDF9), and follicle-stimulating hormone β subunit (FSHB) are essential genes related to goat reproduction (Ghoreishi et al, 2019;Lan et al, 2003;Qi et al, 2019;Zi et al, 2020), and they are necessary growth factors in the ovary to initiate follicle development and regulate ovulation (Kumar et al, 2007). BMPR-1B affects the differentiation of granulosa cells and follicle development and can promote ovulation (Davis et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

TIMP1 may affect goat prolificacy by regulating biological function of granulosa cells

et al. 2022

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Abstract. Tissue inhibitor of metalloproteinase 1 (TIMP1) is associated with animal reproductive processes, such as follicular growth, ovulation, luteinization, and embryo development in mammals. The purposes of this study were to explore the expression and localization of TIMP1 in the ovarian tissues and determine the effect of TIMP1 on the function of granulosa cells and the association of TIMP1 with lambing-related genes of the goats. Immunohistochemical analysis showed that TIMP1 protein was strongly expressed by granulosa cells. Enzyme-linked immunosorbent assay (ELISA) results showed that TIMP1 overexpression promoted the secretion of estradiol of granulosa cells after 12, 24, and 48 h of transfection. Moreover, in vitro experiments indicated that TIMP1 had the ability to promote the cell proliferation and elevate the transcriptional levels of four genes associated with goat prolificacy, including BMPR-1B, BMP15, GDF9, and FSHB, in granulosa cells. In conclusion, TIMP1 could be an important molecule in regulating reproductive performance of the goats by affecting estrogen secretion and cell proliferation, as well as the expression of lambing-related genes of granulosa cells in the goats.

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Comparison of the sequences and expression levels of genes related to follicular development and atresia between prolific and nonprolific goat breeds

Cited by 7 publications

References 55 publications

Analysis on the desert adaptability of indigenous sheep in the southern edge of Taklimakan Desert

Analysis on the desert adaptability of indigenous sheep in the southern edge of Taklimakan Desert

Reproductive characteristics of Saanen and Alpine bucks

TIMP1 may affect goat prolificacy by regulating biological function of granulosa cells

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