Genotyping of Low β-ODAP Grass Pea (Lathyrus sativus L.) Germplasm with EST-SSR Markers

Abstract

Grass pea (Lathyrus sativus L.) is an important protein source in arid regions as both human and animal food. Despite its significance, the use of grass pea is limited by the presence of β-N-oxalyl-L-a,bdiaminopropionic acid (β-ODAP) which can cause neurological disorders. Breeding studies in grass pea have therefore focused on developing high-yielding varieties with low β-ODAP content. However, the narrow range of genetic diversity and the restricted genomic tools in grass pea have slowed progress in such breeding. The present investigation was conducted to explore the genetic diversity of low β-ODAP germplasm consisting of 22 accessions with 31 EST-SSR markers. The molecular analyses revealed a total of 133 alleles ranging from 142 to 330 bp with a mean number of alleles per locus of 4.29. The mean polymorphic information content (PIC) value was calculated as 0.49, and the EST-SSRs in loci S5, S6 and S116 were of the most informative PICs. A dendrogram based on Nei's genetic distance matrix revealed that breeding lines were grouped in two main clusters. Genetic distances were higher between GP6/GP11, GP4/GP11 and GP5/GP8 accessions which could be further used in crop improvement studies for developing wider genetic diversity.

Morpho-molecular genetic diversity and population structure analysis in garden pea (Pisum sativum L.) genotypes using simple sequence repeat markers

“…The clustering of the genotypes indicated no parallelism between genetic diversity and geographical diversity, since the genotypes of various geographic regions were grouped in different clusters. Similar results were reported in pea by earlier workers [52] who reported that 35 pea accessions were grouped into two major clusters by using 15 polymorphic SSR markers, Wang et al [47] found that 10 species of Lathyrus genus clustered into two major groups, Arslan et al [53] have found two major groups of 22 grass pea genotypes. The result of cluster analysis based on the morphological and molecular markers was not similar and it may be due to the environmental influence on the morphological traits.…”

Deciphering Genetic Diversity in Grass Pea (Lathyrus sativus L.) Collections Using Agronomic and Forage Quality Traits and SSR Markers

“…Out of the 27 primer pairs screened, 11 (40.7%) were polymorphic compared to 33% polymorphic primers reported in Medicago (Chandra, 2011). The average number of alleles obtained in our collections (5.36) was higher than 4.0 and 3.2 reported by previous studies (Gupta et al, 2018) and lower than alleles reported in a diverse collection of grass pea (Wang et al, 2015;Arslan et al, 2020). These differences in the alleles might be due to genotypes collected from diverse geographical regions.…”

“…Several studies have been conducted to assess genetic diversity using morphological and molecular markers in range legumes Majidi, 2015, Irani et al, 2016). However, such efforts in grass pea were mainly related with ODAP levels and seed yield using agromorphological traits, biochemical and molecular markers (Wang et Arslan et al, 2020). However, only few studies investigated the forage nutritional aspects of grass pea (Basaran et al, 2011).…”

Rediscovering the Potential of Multifaceted Orphan Legume Grasspea- a Sustainable Resource With High Nutritional Values

“…In future improvement programmes, the use of molecular markers for the genetic diversity studies and their utilization in markertrait association for plant phenology and yield-related traits are expected to play a crucial role in understanding the association of novel alleles in trait expression (116,117). The development and use of simple sequence repeats (SSRs) (45,(118)(119)(120), EST-SSR (111), Restriction Fragment Length Polymorphism (RFLP), and Random Amplified Polymorphic DNA (RAPD) (10,121,122) markers as a conventional molecular tool and recent development of SSR markers by In silico mining of nucleotide sequences (117) has enhanced our understanding in genetic linkage mapping, QTL mapping, association mapping, DNA fingerprinting and genetic diversity studies. The above mentioned research on the topics related to grasspea breeding has given a promising way of exploring the genetic potential of this species.…”

“…This will allow for the development of genetic novelties with intriguing agronomic properties such as stress tolerance and rusticity from grasspea and grain quality from peas ( 110 ). The use of genetically distant grasspea accessions could give possible superior recombination with low β-ODAP content compared to carrying out crosses among or between the genetically closer species ( 111 ).…”