Genotyping of Kappa-Casein Gene of Buffalo in Indonesian

Margawati, Endang Tri; Volkandari, Slamet Diah; Indriawati,; Talib, Chalid

doi:10.14334/proc.intsem.lpvt-2016-p.37-44

Cited by 7 publications

(4 citation statements)

References 11 publications

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“…PCR-RFLP results using AcuI enzyme disagreed with the majority of reviewed PCR-RFLP studies conducted by HindIII , HinfI or MaeII restriction enzymes which concluded that, all Egyptian buffaloes were monomorphic and genotyped as homozygous "BB" animals as reported by Othman (2005); El-Rafey and Darwish (2008); and Abdel Dayem et al (2009). Similar results were also noticed, in Pakistani buffalo (Riaz et al, 2008;Ghafoor et al, 2015), in Indian buffalo (Gangaraj et al, 2008;Shende et al, 2009;Nair et al, 2011;George et al, 2018), in Brazilian buffalo (Otaviano et al, 2005), in Chinese water buffalo (Ren et al, 2011) , in Iraqi buffalo (Jaayid et al, 2013), in Anatolian water buffalo breed (Cinar et al, 2016), in swamp and river Indonesian buffaloes (Margawati et al, 2016), in Cuban buffalo (Marrero et al, 2016), and in Iranian buffalo (Abbasi et al, 2009).…”

Section: Detection Of Polymorphism and Genetic Variants Of κ-Cn And β-Lgsupporting

confidence: 53%

Polymorphism, Allelic and Genotypic Frequencies of κ-Casein and β-LG genes in Egyptian Buffaloes

Al-Shawa¹,

El-Zarei²,

Ghazy³

et al. 2020

JAS

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With a view to detecting the genotypes of both κ-CN and β-LG genes in native populations of Egyptian buffalo using PCR-RFLP technique, 80 randomly, individuals were selected from five geographical locations of some Egyptian provinces. Also, to estimate the population genetic parameters such as, allelic and genotypic frequencies, heterozygosity, and inbreeding coefficient (F IS ) of these studied genes. For genotyping, 453 bp PCR product of κ-CN was digested with AcuI and HpyCH4IV (Isoschizomer for MaeII) restriction enzymes while the 247 bp PCR product of β-LG was digested with HaeIII restriction enzyme. PCR-RFLP results discovered polymorphism at the level of κ-CN gene in all studied Egyptian buffaloes with two distinct alleles "A" and "B". PCR-RFLP analysis for κ-CN gene using both restriction enzymes successfully detected that polymorphic status of the studied populations. We recommended using AcuI enzyme which was more capable for differentiating between homozygous (17%) and heterozygous (83%) individuals than HpyCH4IV enzyme which defined only 4% of homozygous individuals and the remaining was heterozygous (96%) individuals. Existence of heterozygosity excess in all studied populations referred to higher degree of genetic variability between individuals within these populations. On contrary, results of PCR-RFLP at the level of β-LG gene revealed a monomorphic pattern of Egyptian buffaloes and genotyped as "AA" animals which signified that PCR-RFLP assay with HaeIII enzyme for β-LG gene failed to discover any evidence of polymorphism in Egyptian buffalo under the circumstances of this study or all studied animals possess only one allele.

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Section: Detection Of Polymorphism and Genetic Variants Of κ-Cn And β-Lgsupporting

confidence: 53%

Polymorphism, Allelic and Genotypic Frequencies of κ-Casein and β-LG genes in Egyptian Buffaloes

Al-Shawa¹,

El-Zarei²,

Ghazy³

et al. 2020

JAS

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“…Many researchers have reported that genotype in kappa casein gene associated with milk yield (Vidović et al, 2013;Deb et al, 2014), milk fat yield (Djedović et al, 2015), and cheese yield (Azevedo et al, 2008). In buffalo, kappa casein gene was observed for milk production traits (Patel et al, 2007b;Margawati et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Genetic polymorphism of kappa-casein gene in Friesian Holstein: a basic selection of dairy cattle superiority

Volkandari

Indriawati

Margawati

2017

J. Indonesian Trop. Anim. Agric.

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ABSTRAKKasein adalah protein susu yang terbagi dalam empat grup yaitu αS1, αS2, β-casein dan kappacasein (CSN3). Gen kappa-kasein berpengaruh terhadap pembentukan komponen dalam susu. Tujuan dari penelitian adalah mengidentifikasi polimorfisme gen kappa-kasein pada sapi perah Friesian Holstein (FH). Sebanyak 59 sampel sapi FH yaitu 32 asal Malang, 10 asal Sukahati Bogor dan 17 asal Pusat Penelitian Bioteknologi-Lembaga Ilmu Pengetahuan Indonesia (LIPI) (ternak koleksi) digunakan dalam penelitian. Sampel DNA diekstraksi dengan metode garam pekat dan dikuantifikasi menggunakan Spektrofotometer. Fragmen DNA spesifik gen kappa-kasein sepanjang 379 pb diamplifikasi menggunakan metode Polymerase Chain Reaction (PCR) dengan suhu annealing 56 o C dan 30 siklus. Teknik PCR-RFLP dengan enzim restriksi HindIII digunakan untuk analisis genotip. Hasil analisis menunjukkan bahwa terdapat tiga varian genotip yaitu AA, AB dan BB pada populasi Malang dan ternak koleksi Puslit Bioteknologi-LIPI sementara dua varian (AA dan BB) ditemukan pada populasi Sukahati. Rata-rata frekuensi genotip AA sebesar 65,28%, AB 31,72% dan BB 3,00% dengan rata-rata frekuensi alel A sebesar 0,81 dan B sebesar 0,19. Populasi sapi FH dalam keseimbangan genetik. Kesimpulan, polimorfisme gen kappa-kasein ditemukan pada ketiga populasi sapi FH dengan frekuensi alel A lebih tinggi dibanding alel B. Alel B diketahui berhubungan dengan produksi susu, komposisi susu dan cheese yield. Peningkatan alel B pada populasi FH berdampak terhadap performans produksi dan komponen susu. Eksplorasi (kuantitatif, kualitatif, dan molekuler) perlu dilakukan untuk aplikasi genetika molekuler pada seleksi sapi perah.Kata Kunci: gen kappa casein, polimorfisme, sapi perah, Friesian Holstein ABSTRACTCaseins are milk protein subdivided into four main groups which are αS1, αS2, β-casein and kappacasein (CSN3). Kappa-casein gene influences the manufacturing of milk properties. The aim of this study was to identify the kappa-casein gene polymorphism in Friesian Holstein (FH) cattle. Fifty nine (59) samples consisted of 32 (Malang), 10 (Sukahati Bogor) and 17 (Research Center for Biotechnology-Indonesian Institute of Sciences's collections) were applied in this study. DNA samples were extracted by high concentrated NaCl and quantified by spectrophotometer. The kappa-casein gene was amplified at 379 bp fragment by PCR method using a pair primer of kappa-casein at 56 o C annealing for 30 cycles. PCR-RFLP technique with HindIII was used for genotyping analysis. The result showed that there were three variants of genotypes (AA, AB and BB) in two populations from Malang and RC for Biotechnology-LIPI's collection while cattle from Sukahati had only AA and AB genotypes. The averages of genotype frequencies were 65.28%; 65.28%; and 3.00% for AA, AB and BB genotypes Polymorphism of Kappa-casein gene in FH cattle (S.D.Volkandari et al.) 213 respectively while frequencies of 0.81 and 0.19 were for A and B alleles, respectively. FH cattle populations were in equilibrium genetics. This finding...

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“…The study of CSN3 gene reported by Margawati et al . [ 84 ] showed that all samples possessed BB genotypes, which merged the pattern of the digested fragments with sizes of 255 and 154 bp. This finding suggests that genotyping using HindIII reveals monomorphic BB types associated with milk content of casein.…”

Section: Development Of Genetic Studies In Indonesian Indigenous Buffalomentioning

confidence: 99%

Biodiversity of Indonesian indigenous buffalo: First review of the status, challenges, and development opportunities

Prihandini,

Tribudi,

Hariyono

et al. 2023

Vet World

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In Indonesia, the buffalo is important for small and marginal farmers’ livelihood and economic development as a source of food, working animal, and tourist attraction. Therefore, an in-depth study is needed to examine challenges and opportunities for buffalo development in Indonesia. In Indonesia, the buffalo is divided into two types: swamp buffalo and river buffalo. The buffalo population in Indonesia has declined significantly. A decrease of approximately 39.35% was recorded from 2022 to 2017. The decline occurred due to low reproduction rate and suboptimal rearing management systems. There are three buffalo-rearing systems: Intensive, semi-intensive, and extensive. The productivity of buffalo is diverse and closely related to the characteristics of the regional agroecosystem, consistent with existing natural resources and rearing management systems. The diversity of buffalo productivity provides a good opportunity to improve productivity. Improvement of buffalo genetics is urgently needed, by improving mating management, etc., especially to reduce potential inbreeding. In recent years, genetic and molecular research on Indonesian buffalo has made progress, including use of molecular markers, such as microsatellites and single-nucleotide polymorphisms, to evaluate genetic diversity within and among buffalo populations across Indonesia. In addition, studies are being conducted on the relationship of genotype mutations that contribute to appearance and phenotypic performance (heat stress, reproduction, behavior, coat color, and production attributes) in buffaloes. Identification of genetic diversity in local buffaloes can be improved using various genetic and genomic techniques. These findings will form a basis for the targeted conservation of local buffaloes in Indonesia. This study aimed to collect information on the genetic resources of the local buffalo, particularly its status and production system and provide recommendations for developing buffalo production in Indonesia. Keywords: Bubalus bubalis, diversity, production system, zoogenetic resources.

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Genotyping of Kappa-Casein Gene of Buffalo in Indonesian

Cited by 7 publications

References 11 publications

Polymorphism, Allelic and Genotypic Frequencies of κ-Casein and β-LG genes in Egyptian Buffaloes

Polymorphism, Allelic and Genotypic Frequencies of κ-Casein and β-LG genes in Egyptian Buffaloes

Genetic polymorphism of kappa-casein gene in Friesian Holstein: a basic selection of dairy cattle superiority

Biodiversity of Indonesian indigenous buffalo: First review of the status, challenges, and development opportunities

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