Genotyping of Indigenous Cattle Breeds and their Exotic Crosses for beta -casein Milk Type using PCR-RFLP and Sequencing

Patel, A. D.; Patel, C. G.; Zala, Hinal; Sheikh, Yasin; Dadheech, Tripti; Mankad, Pooja; Shah, S. V.; Rank, D. N.

doi:10.5455/ijlr.20190216104000

Cited by 3 publications

(4 citation statements)

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“…In AS-PCR genotyping, all individuals possess a common frag- It is notable to mention that limited information is available on Bos taurus × Bos indicus crossbred cattle for comparison with present results. Similar to our findings, Patel et al (2019) reported that the frequencies of A1A1, A1A2 and A2A2 genotypes were 0.09, 0.56 and 0.34, respectively in the HF × Kankrej crossbred population and the corresponding A1 and A2 allelic frequency were 0.38 and 0.62, respectively. Likewise, Malarmathi et al (2014) reported the proportion of A1A1, A1A2 and A2A2 genotypes to be 0.17, 0.46 and 0.37, respectively, in HF crossbred cattle of Tamil Nadu, India.…”

Section: Genotyping Of A1 and A2 Allelic Variantssupporting

confidence: 91%

“…Similarly, Sodhi et al (2018) reported the frequencies of A1A2 and A2A2 genotypes to be 0.21 and 0.79, respectively, in Ladakhi cattle. However, Patel et al (2019) identified three different genotypes as A1A1 (0.01), A1A2 (0.34) and A2A2 (0.65) in Gir cattle of India while the corresponding A1 allele frequency (0.18) was found much higher than in the earlier reports. In addition, previous reports mentioned the existence of low frequent A1 allele in different zebu populations that ranged between 0.00 and 0.11 in Ongole, Malanad Gidda, Kherigarh, Kasargod, Achai and Lohani (Mishra et al 2009;Ganguly et al 2013;Ramesha et al 2016;Ayaz et al 2022) that support the present findings.…”

Section: Sequence Characterization Of Csn2 Genecontrasting

confidence: 67%

“…Different methods like PCR-RFLP, AS-PCR and sequencing have been employed for genotyping of the most frequent A1 and A2 allelic variants of CSN2 gene (Dai et al 2016;Massella et al 2017;Kumar et al 2019;Patel et al 2019). Among them, AS-PCR is one of the rapid, cost effective and precise methods for the screening of A1 and A2 genotypes in different cattle populations worldwide.…”

Section: Genotyping Of A1 and A2 Allelic Variantsmentioning

confidence: 99%

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Molecular characterization and A1/A2 genotyping of casein beta gene in zebu and crossbred cattle of Bangladesh

Pabitra¹,

Maruf²,

Mony³

et al. 2022

CZECH J ANIM SCI

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Casein beta (CSN2) is the most explored gene in cattle due to its potential impact on human health. Here, we investigated the entire coding sequence of CSN2 gene except the last two codons for detection of polymorphisms in different cattle populations of Bangladesh as well as genotyping of A1 and A2 allelic variants using a newly developed allele specific polymerase chain reaction (AS-PCR) based the genotyping protocol. Five primer pairs were used to amplify the coding sequences of CSN2 gene. This study included a total of 258 DNA samples from six Bangladeshi zebu populations and one Holstein Friesian (HF) derived crossbred cattle population. Sequence analysis detected five nonsynonymous mutations in the coding sequence of CSN2 gene that defined five allelic variants as A1, A2, B, F and I. In addition, substitution of GTA (Val) by GCA (Ala) at position 197 resulted in an undefined allele in the zebu cattle population of Bangladesh that has not yet been reported elsewhere. Like other Bos indicus cattle populations, A2 allele is predominant in the studied zebu populations. The mean frequencies of A1A1, A1A2 and A2A2 genotypes were 0.02, 0.16 and 0.82, respectively, in zebu cattle populations while the corresponding allele frequencies were A1 (0.10) and A2 (0.90). In opposite, the aforesaid genotype frequencies were 0.14 (A1A1), 0.50 (A1A2) and 0.36 (A2A2) in the HF crossbred population with allele frequencies of 0.39 (A1) and 0.61 (A2). The adopted AS-PCR method was found cost-effective, rapid and had high specificity for genotyping of A1 and A2 allelic variants. Altogether, this study provides information for the selection of desired zebu and crossbred individuals in order to produce premium quality milk as well as to design a breeding plan in the crossbreeding program.

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Section: Genotyping Of A1 and A2 Allelic Variantssupporting

confidence: 91%

Section: Sequence Characterization Of Csn2 Genecontrasting

confidence: 67%

Section: Genotyping Of A1 and A2 Allelic Variantsmentioning

confidence: 99%

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Molecular characterization and A1/A2 genotyping of casein beta gene in zebu and crossbred cattle of Bangladesh

Pabitra¹,

Maruf²,

Mony³

et al. 2022

CZECH J ANIM SCI

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“…Acta fytotechn zootechn, 26, 2023(1): 39-45 http://www.acta.fapz.uniag.sk and Kankrej, the allele frequency are 0.82 and 0.92, respectively. In crossbreeds of the first generation with Holstein sires, the frequency of the A2 allele was, on average, 0.63 (Patel et al, 2019;Gorkhali et al, 2020).…”

Section: Identification Of Polymorphism Of the Csn2 Gene Encoding Bet...mentioning

confidence: 99%

Identification of Polymorphism of the CSN2 Gene Encoding Beta-Casein in Ukrainian Black and White Breeds of Cattle

Skliarenko

2023

Acta fytotechn zootechn

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Licensed under a Creative Commons Attribution 4.0 International License Genotyping of 606 animals of the Ukrainian Black-and-White dairy breed was carried out in order to establish the features of genotype formation by beta-casein. It is established that the modern population of this breed has been formed using stud bulls of the Holstein breed, mainly of American and German breeding. It is found that the original breed has determined the features of polymorphism of the beta-casein gene. According to the research results, it is proved that the existing genetic structure of stud bulls of the Holstein and Ukrainian Black-and-White dairy breeds enables the formation of populations homozygous by these characteristics in subsequent generations. Among the breeding stock of the Ukrainian Black-and-White dairy breed, the frequency of the desired A2A2 genotype was 33-53%. It is expected to increase the frequency of occurrence of individuals with the A2A2 genotype by beta-casein in subsequent generations, especially if stud bulls homozygous by A2A2 beta-casein is used.

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Demographic pattern of A1/A2 beta casein variants indicates conservation of A2 type haplotype across native cattle breeds (Bos indicus) of India

et al. 2022

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Genotyping of Indigenous Cattle Breeds and their Exotic Crosses for beta -casein Milk Type using PCR-RFLP and Sequencing

Cited by 3 publications

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Molecular characterization and A1/A2 genotyping of casein beta gene in zebu and crossbred cattle of Bangladesh

Molecular characterization and A1/A2 genotyping of casein beta gene in zebu and crossbred cattle of Bangladesh

Identification of Polymorphism of the CSN2 Gene Encoding Beta-Casein in Ukrainian Black and White Breeds of Cattle

Demographic pattern of A1/A2 beta casein variants indicates conservation of A2 type haplotype across native cattle breeds (Bos indicus) of India

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