Genotyping of ESBL Producing UropathogenicEscherichia coliin West of Iran

Abstract

Background and Objective. Urinary tract infection (UTI) is one of the most common bacterial infections in the world. Molecular fingerprinting of UTI isolates such as pulsed-Field Gel Electrophoresis using for Clonal distribution and determine of predominant type. The aim of the study was to determine genotyping of ESBL producing UPECs. Material and Methods. 200 UPEC isolates from outpatients with UTI were obtained. Antimicrobial susceptibility and interpretation were performed by disk diffusion. Virulence factors for UPECs were screened by using PCR. UPECs were analyzed by Pulsed-Field Gel Electrophoresis and images analyzed by Phoretix1DPro software. Results. A total of 200 isolates of UPECs, 24.5% (n = 49) of isolates, were positive for ESBL production. Resistance ranged from 0% for amikacin and imipenem to over 93.9% for carbenicillin and ampicillin. Frequencies of haemagglutination, haemolysin, and hydrophobicity were 51%, 18.3%, and 14.28%, respectively. A total of 10 different genotypes were obtained, which include nine common clones and one single clone. Conclusion. We confirmed the prevalence of virulence phenotyping especially Haemagglutination among UPEC strains and that it can also contribute to virulence in these strains. Large diversity in genotypes was observed in the isolates that could be indicative of different sources of infection in community acquired.

The role of parC, parE, and qnrB Genes in Ciprofloxacin-Resistant Escherichia coli Isolates from Urinary Tract Infections

“…Moreover, Mohajeri et al introduced piperacillin and ampicillin as resistant antibiotics and imipenem and nitrofurantoin as sensitive drugs (25). This finding was in line with the results of the present study; however, minor differences in the resistance level to other antibiotics were observed.…”

“…Mohajeri et al also reported the resistance rate of E. coli isolates to ciprofloxacin as 43% (25), which was lower than the resistance rate reported in the present study (77.5%). This could be attributed to the increasing emergence of resistant bacteria to wide-spectrum antibiotics.…”

Relationships between Virulence Factors and Antimicrobial Resistance among <italic>Escherichia coli</italic> Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran

“…Although the spectrum and frequency of antibiotic resistance among UPEC isolates is variable [ 24 – 26 ], most studies report that amikacin, nitrofurantoin and imipenem are highly efficacious against such strains [ 27 – 29 ]. Our study revealed co-resistance to third generation cephalosporins, quinolones, and aminoglycoside among UPEC isolates; this is similar to that of the study by Karlowsky et al [ 30 ], which also reported a high degree of co-resistance to ampicillin and SXT in UPEC isolates.…”

Multidrug- and Extensively Drug-Resistant Uropathogenic Escherichia coli Clinical Strains: Phylogenetic Groups Widely Associated with Integrons Maintain High Genetic Diversity

“…These data support the hypothesis that the TosA protein is a potential virulence marker in UPEC strains (Vigil et al, 2011a). Nonetheless, ESBL-producing clones associated with phylogenetic group B2 were only localized into the 532U clonal group of cluster III; likewise, the antibiotic resistance profiles showed that resistance to penicillin, beta-lactams, and cephalosporin was included in clonal group B2 (Mohajeri et al, 2014). …”