Genotyping of a nosocomial outbreak of pandemic influenza A/H1N1 2009

Nosocomial vs community-acquired pandemic influenza A (H1N1) 2009: a nested case–control study

“…1,7 Molecular genotyping can help describe the outbreaks of nosocomial pH1N1, although its wider applicability requires further assessment. 27 Finally, it is important that all available preventive measures, whether pharmaceutical or non-pharmaceutical, are used to prevent nosocomial influenza in children with atrisk medical conditions. These include annual influenza vaccination of eligible children (children aged six months or more with a chronic medical condition predisposing to severe influenza illness that requires regular medical follow-up or hospitalization), vaccination of HCWs, use of personal protective measures, hand hygiene and tight hospital infection control measures.…”

Sequence-based identification and characterization of nosocomial influenza A(H1N1)pdm09 virus infections

“…Although characterization of nosocomial A(H1N1)pdm09 cases by a combined molecular and epidemiological approach has been reported, the evaluation of this approach to guide the identification of nosocomial influenza clusters to our knowledge has not previously been reported. 16,17 The present analysis identified nine nosocomial influenza infections during the 2009 influenza epidemic in a tertiary referral hospital, which comprises 15% of all the diagnoses during the study period. Although transmission from an index case to a hospitalized patient was observed four times, no onward transmissions could be demonstrated.…”

Nosocomial transmission of influenza: A retrospective cross‐sectional study using next generation sequencing at a hospital in England (2012‐2014)

“…Previous applications of next generation sequencing of influenza have included elucidating zoonosis and describing transmission of seasonal and pandemic strains . In the context of nosocomial transmission, several studies have used next generation sequencing to assess differences between sequences of specific influenza genome segments (HA, NA and/or PB2) or to investigate small outbreaks . These results have highlighted the importance of multiple introductions of community strains.…”