Nosocomial transmission of influenza: A retrospective cross‐sectional study using next generation sequencing at a hospital in England (2012‐2014)

Blackburn, Ruth; Frampton, Dan; Smith, Catherine M.; Fragaszy, Ellen; Watson, Simon J.; Ferns, R. Bridget; Binter, Špela; Coen, Pietro G.; Grant, Paul; Shallcross, Laura; Kozlakidis, Zisis; Pillay, Deenan; Kellam, Paul; Hué, Stéphane; Nastouli, Eleni; Hayward, Andrew; Kinghorn, Jane; Wurie, Fatima; Rahman, Saadia; Johnson, Anne; Dunn, David; Leigh-Brown, Andrew J.; Morris, Steven; Irving, William L.; Clark, Duncan A.; Zambon, Maria

doi:10.1111/irv.12679

Cited by 23 publications

(16 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For influenza, WGS offer superior resolution for molecular epidemiology compared to single segment analysis [193] . In a recent report from U.K, WGS data confirmed nosocomial transmission for approximately 16% of cases [194] . Equally to the impact of DNA-techniques on criminology, outbreak investigations need to include and integrate laboratory data with epidemiologic data to obtain full value [195,196] .…”

Section: Outbreak Analysismentioning

confidence: 93%

Extensive Hospital In-Ward Clustering Revealed By Molecular Characterization of Influenza A Virus Infection

Sansone

Andersson

Gustavsson

et al. 2020

Clinical Infectious Diseases

View full text Add to dashboard Cite

Background Nosocomial transmission of influenza A virus (InfA) infection is not fully recognized. The aim of this study was to describe the characteristics of hospitalized patients with InfA infections during an entire season and to investigate in-ward transmission at a large, acute-care hospital. Methods During the 2016–17 season, all hospitalized patients ≥18 years old with laboratory-verified (real-time polymerase chain reaction) InfA were identified. Cases were characterized according to age; sex; comorbidity; antiviral therapy; viral load, expressed as cycle threshold values; length of hospital stay; 30-day mortality; and whether the InfA infection met criteria for a health care–associated influenza A infection (HCAI). Respiratory samples positive for InfA that were collected at the same wards within 7 days were chosen for whole-genome sequencing (WGS) and a phylogenetic analysis was performed to detect clustering. For reference, concurrent InfA strains from patients with community-acquired infection were included. Results We identified a total of 435 InfA cases, of which 114 (26%) met the HCAI criteria. The overall 30-day mortality rate was higher among patients with HCAI (9.6% vs 4.6% among non-HCAI patients), although the difference was not statistically significant in a multivariable analysis, where age was the only independent risk factor for death (P < .05). We identified 8 closely related clusters (involving ≥3 cases) and another 10 pairs of strains, supporting in-ward transmission. Conclusions We found that the in-ward transmission of InfA occurs frequently and that HCAI may have severe outcomes. WGS may be used for outbreak investigations, as well as for evaluations of the effects of preventive measures.

show abstract

Section: Outbreak Analysismentioning

confidence: 93%

Extensive Hospital In-Ward Clustering Revealed By Molecular Characterization of Influenza A Virus Infection

Sansone

Andersson

Gustavsson

et al. 2020

Clinical Infectious Diseases

View full text Add to dashboard Cite

show abstract

“…In 2017/8 this was estimated to be 15 % of influenza cases diagnosed during the influenza season. A recent study using phylogenetic analysis of influenza isolates demonstrated genetic clustering in 15.8 % of hospital influenza cases indicating nosocomial acquisition [ 19 ]. Nosocomial acquisition of influenza has knock-on effects in terms of morbidity and delays to discharge, the financial impact of which has not been clarified and further studies are needed to understand the economic effects of these aspects in more detail.…”

Section: Discussionmentioning

confidence: 99%

Point of care testing of Influenza A/B and RSV in an adult respiratory assessment unit is associated with improvement in isolation practices and reduction in hospital length of stay

Berry

Lansbury

Gale

et al. 2020

Journal of Medical Microbiology

View full text Add to dashboard Cite

Introduction. Every winter seasonal influenza and other viral respiratory infections increase pressure on the health services and are associated with nosocomial infection and morbidity. Aim. To compare provision of point-of-care (POC) testing with laboratory-based testing for influenza and RSV detection on an adult respiratory assessment unit to assess the impact on isolation practices and length of stay (LOS). Methodology. Prospective interrupted ‘on-off’ study in adults admitted to the respiratory unit between December 2018 and April 2019 with a suspected respiratory tract infection. Nasopharyngeal samples were tested using either the GeneXpert rapid POC test for influenza and RSV (on-period), or were sent to the laboratory for multiplex PCR testing against a panel of 12 respiratory viruses (off-period). Outcome measures were time to patient isolation for infection control, LOS and turnaround time from admission to test results. Results. Of 1145 patients evaluated, 755 were tested with POC and 390 with laboratory multiplex; a respiratory virus was identified in 164 (21.7 %) and 138 (35.4 %) patients respectively. A positive POC test was associated with a shorter time to isolation (mean difference 16.9 h, P <0.001), shorter LOS (mean difference 15.5 h, P =0.05,) and shorter turnaround time (mean difference 28.3 h, P <0.001), compared to laboratory testing. Conclusion. Use of GeneXpert POC testing for Flu/RSV is associated with rapid reporting of results with significant improvements in isolation practices and reductions in LOS.

show abstract

“…Algunas soluciones convencionales como limpiadores de aires se encuentran bajo evaluación para probar su capacidad en escenarios relevantes [4], sin embargo, esos sistemas no han sido probados en entornos tales como hospitales, con carga viral confirmada de SARS-CoV2. La diseminación de patógenos infecciosos en hospitales puede ser significativa y, en varios escenarios, el requisito es contar con un ambiente libre de patógenos (por ejemplo, en quirófanos) no sólo para protección de pacientes sino también del personal del hospital [5]. Se ha demostrado que la generación de iones negativos puede reducir la transmisión de enfermedades debido a patógenos como virus [6] y bacterias [7].…”

Section: Planteamiento Del Problemaunclassified

Dispositivo Basado en Ionización Para Limitar La Transmisión Aérea Del Covid-19

Ramírez

Baltazar²,

Cureño³

et al. 2021

Epistemus

View full text Add to dashboard Cite

Esta investigación busca demostrar la efectividad de un dispositivo de ionización de aire para limitar la transmisión aérea del COVID-19. Para probar la efectividad del dispositivo, primero se probó en un ambiente controlado y después en una institución de salud pública con casos confirmados de COVID-19. Algunos microorganismos (no incluido SARS-CoV-2) fueron diseminados en el ambiente controlado, donde se colocaron marcadores en diferentes lugares para verificar el comportamiento en condiciones normales y después bajo la influencia del dispositivo para comparar la carga ambiental de microorganismos. Una aproximación similar se utilizó en la institución de salud, donde el SARS-CoV-2 fue primeramente confirmado colocándose después el dispositivo. Los resultados muestran que, en el ambiente controlado, el dispositivo puede reducir la carga de microorganismos hasta en un 99% en 1/6 del tiempo mientras que, en la institución de salud, la carga viral se puede reducir en hasta el 80% en pocos minutos.

show abstract

Nosocomial transmission of influenza: A retrospective cross‐sectional study using next generation sequencing at a hospital in England (2012‐2014)

Cited by 23 publications

References 29 publications

Extensive Hospital In-Ward Clustering Revealed By Molecular Characterization of Influenza A Virus Infection

Extensive Hospital In-Ward Clustering Revealed By Molecular Characterization of Influenza A Virus Infection

Point of care testing of Influenza A/B and RSV in an adult respiratory assessment unit is associated with improvement in isolation practices and reduction in hospital length of stay

Dispositivo Basado en Ionización Para Limitar La Transmisión Aérea Del Covid-19

Contact Info

Product

Resources

About