Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers

Abstract

We examined 18 combinations of SRAP primers with resistance gene analog (RGA) and chitinase degenerate primers in order to determine their utility for genotyping L. angustifolius. Primer pairs ResAn51-f/Me8, p-loop/Em5, TM/Me8, Chit3-r/Em5 were the most effective for detection of genetic polymorphism of different narrow-leaved lupine varieties.