Genotyping and Phenotyping of the Potential Clones, Biotypes and Variants of Grapevine Cultivar Korinthiaki Staphis (Vitis vinifera L.)

Abstract

This study presents the results regarding the identification and discrimination of twenty seven possible clones of grapevine cultivar Korinthiaki staphis (Vitis vinifera L.), three biotypes of Korinthiaki lefki and the related cultivar Staphidampelo using the ampelographic description and the molecular method AFLP. The results from the statistical analysis showed that all the biotypes of cultivar Korinthiaki staphis show small distance and are grouped in the same cluster, depending on their origin, while Staphidampelo and Korinthiaki lefki are neither variants nor biotypes of the cultivar Korinthiaki staphis but different cultivars since they are very distant compared to the other biotypes and moreover, Korinthiaki lefki is in a separate cluster of the dendrogram. The ampelographic description in combination with the molecular method AFLP are effective for the study of the between and within genetic diversity of grapevine cultivars as well as for their identification and discrimination. The results of this study can constitute the base for the implementation of the clonal selection for grapevine cultivar Korinthiaki staphis and the seclusion of the desired clones.

Morphometric and Molecular Analysis of the Three Arbutus Species of Greece

“…However the suggested descriptors in combination with molecular markers proved a useful tool to assess the hybrid identity of these individuals. Combination of morphological traits with RAPD has been successfully used for differentiation of other natural hybrids and clones, too (Yüzbaşıoğlu et al, 2008;Stavrakaki and Biniari, 2016). The results of this research are supported by the fact that plants derived by micropropagation of individuals with intermediate characteristics defined as A.…”

Polyphenol content and antioxidant capacity of the skin extracts of berries from seven biotypes of the Greek grapevine cultivar Korinthiaki Staphis (Vitis vinifera L.)

“…Due to its ancient lineage and cultivation, Korinthiaki Staphis goes by numerous synonyms (Guillon, 1895;Molon, 1906;Viala and Vermorel, 1909;Krimbas, 1943;Davidis, 1982;Vlachos, 1986;Stavrakakis, 2010;Robinson et al, 2012). Its polyclonal synthesis has been confirmed by a study relying on the ampelographic description and the AFLP molecular method through the identification/discrimination of twenty (20) biotypes/possible clones (Stavrakaki and Biniari, 2016). Most studies investigating the polyphenolic content of grapevine varieties turn to plant material -grapes, wines, raisins-usually originating in productive vineyards where several clones of usually polyclonal varieties are cultivated together.…”

“…All biotypes were identified by means of the ampelographic description and of molecular methods. The experimental vineyard, where the biotypes are preserved and where form the samples were collected (Stavrakaki and Biniari, 2016), is located in Nemea (alt: 195-200 m), northeastern Peloponnese, Greece. The seven-year-old vines were all grafted on rootstock 1103 Paulsen; were bilateral cordontrained (bilateral Royat) at 2.2m x 1.2m intervals; and were spur-pruned to 2-node spurs.…”