Genotyping and identification of six date palm (Phoenix dactylifera L.) cultivars<br>of the Gaza Strip by random amplification of polymorphic DNA

Abstract

Date palm (Phoenix dactylifera L.) is one of the major fruit crops in the Gaza Strip. The main cultivars are Hayani, Bentaisha, Barhi, Zahedi, Ameri and Halawy. Because of the difficulty associated with morphological identification of date palm cultivars, development of cultivar specific genetic markers have been extensively studied. The purpose of this study was to optimize and apply a reliable molecular marker protocol for genotyping and identification of these cultivars. Random amplification of polymorphic DNA (RAPD) was used to study the genetic diversity among the six cultivars using 42 primers. Genetic similarity matrices were constructed for the six cultivars using the Nei and Li formula and clustered with the UPGMA to determine the relationships between the six cultivars. Nine primers were informative and reproducible, and gave a number of polymorphic bands (on average, 7.2 bands per primer). The overall level of polymorphism was 49%. Genetic similarity among the six cultivars ranged from 76.3 to 93.5%. Some primers gave reproducible cultivar-specific bands that may be reliable to identify that cultivar. Our study introduces for the first time a molecular marker approach, capable of distinguishing and studying the genetic diversity among the six date palm cultivars in the Gaza Strip.

The current status of the date palm (Phoenix dactylifera) and its uses in the Gaza Strip, Palestine

“…The 19 cultivars of date palm grown in the Gaza Strip are very difficult to be distinguished by morphology as was indicated by El Kichaoui et al (2013). They are mostly distinguished by the characters of the fruits, produced only after 4-5 years as indicated by Sedra et al (1998).…”

“…They revealed many threats facing the local cultivation of date palms, which is more concentrated in the Middle Governorate of the Gaza Strip. El Kichaoui et al (2013) described the genetic variation and molecular characterization of six date palm cultivars in the Gaza Strip. More recently, El-Hindi (2017) and El Kichaoui et al (2017) investigated the evaluation, isolation and molecular identification of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana against the RPW in the Gaza Strip.…”

EKSTRAKSI MINYAK DARI BIJI KURMA (Phoenix dactylifera L.) DENGAN METODE SOXHLET EXTRACTION DENGAN MENGGUNAKAN ETIL ASETAT

“…Biji kurma berpotensi sebagai edible oil [11]. Terdapat lebih dari 3000 varietas kurma di seluruh dunia [18]. Negara penghasil kurma terbanyak yaitu Mesir, Arab Saudi, Iran, Uni Emirat Arab, Aljazair, Irak, Pakistan, Oman, Tunisia dan Libia [24].…”

Evaluation of molecular diversity of central European maize cultivars

“…RAPD technique requires only small amounts of DNA sample without involving radioactive labels and are simpler as well as faster. RAPD has proven to be quite efficient in detecting genetic variations and used for diversity assessment and for identifying germplasm in a number of plant species (Gajeraa et al, 2010;El Kichaoui et al, 2013;Srivashtav et al, 2013;Omalsaad et al, 2014;Vivodík et al, 2014;Žiarovská et al, 2014). Suitability of RAPD markers for the construction of genetic maps, fingerprinting and phylogenetic studies has been proved by many authors.…”