Evaluation of molecular diversity of central European maize cultivars

Balážová, Želmí­ra; Vivodík, Martin; Gálová, Zdenka

doi:10.9755/ejfa.2015-05-204

Cited by 5 publications

(1 citation statement)

References 16 publications

(25 reference statements)

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“…These features, coupled with their ease of detection, make them ideal for identifying and distinguishing between accessions that are genetically very similar (Saker et al, 2005). For the analysis of genetic diversity of maize genotypes were used several dominant molecular markers: amplified fragment length polymorphism (AFLP) (Roy and Kim, 2016), random amplified polymorphic DNA (RAPD) (Balážová et al, 2016;Vivodík et al, 2017a), start codon targeted (SCoT) (Vivodík et al, 2017b), inter-simple sequence repeat (ISSR) (Žiarovská et al, 2013) and sequence-related amplified polymorphism (SRAP) (Abd El-Azeem et al, 2015). And codominant molecular markers were also used for the analysis of maize genotypes: simple sequence repeat (SSR) (Shiri et al, 2014), expressed sequence tag (EST)-SSR (Galvão et al, 2015) and using protein markers (SDS-PAGE) (Vivodík et al, 2017c).…”

Section: Introductionmentioning

confidence: 99%

Molecular Variability of Old Maize (Zea Mays L.) Based on Gene Specific Markers

Vivodík¹,

Petrovičová²,

Balážová³

et al. 2019

Agrobiodiversity for Improving of Nutrition, Health and Life Quality

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Section: Introductionmentioning

confidence: 99%

Molecular Variability of Old Maize (Zea Mays L.) Based on Gene Specific Markers

Vivodík¹,

Petrovičová²,

Balážová³

et al. 2019

Agrobiodiversity for Improving of Nutrition, Health and Life Quality

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Genetic diversity of maize resources revealed by different molecular markers

Chňapek,

Balážová,

Špaleková

et al. 2024

Genet Resour Crop Evol

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Maize (Zea mays L.) is the third most important cereal crop in the world because of its nutritional value and industrial benefits. Molecular markers are used mainly by the breeders to study the genetic variability of genotypes and its application in the breeding process. Two types of molecular markers, 10 random amplified polymorphic DNA (RAPD) primers and 10 start codon target (SCoT) primers, were assayed to determine the genetic diversity of 25 Slovak maize lines and 25 maize cultivars. A high level of polymorphism was found with both RAPD and SCoT markers, which was confirmed by high average polymorphism information content (PIC) values using both techniques. The efficiency of individual marker techniques in the detection of genotype diversity can be compared by calculating the marker index (MI), detecting diversity index (DDI), discriminating power, resolving power (RP) and other indices. A higher MI (11.788), DDI (2.358) and RP (53.08) value was achieved by the SCoT technique compared to the RAPD method. Three joint dendrograms and PCoA plots constructed based on RAPD, SCoT and both methods combined confirmed the unambiguous separation of maize lines and cultivars from each other. The results obtained from the RAPD and SCoT analysis can be used for the selection of potentially suitable biological sources for further marker-assisted breeding.

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Start codon targeted (scot) polymorphism reveals genetic diversity in european old maize (Zea mays L.) Genotypes

Vivodík

Gálová

Balážová

et al. 2016

Potr. S. J. F. Sci.

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Maize (Zea mays L.) is one of the world's most important crop plants following wheat and rice, which provides staple food to large number of human population in the world. It is cultivated in a wider range of environments than wheat and rice because of its greater adaptability. Molecular characterization is frequently used by maize breeders as an alternative method for selecting more promising genotypes and reducing the cost and time needed to develop hybrid combinations. In the present investigation 40 genotypes of maize from Czechoslovakia, Hungary, Poland, Union of Soviet Socialist Republics, Slovakia and Yugoslavia were analysed using 20 Start codon targeted (SCoT) markers. These primers produced total 114 fragments across 40 maize genotypes, of which 86 (76.43%) were polymorphic with an average of 4.30 polymorphic fragments per primer and number of amplified fragments ranged from 2 (SCoT 45) to 8 (SCoT 28 and SCoT 63). The polymorphic information content (PIC) value ranged from 0.374 (ScoT 45) to 0.846 (SCoT 28) with an average of 0.739. The dendrogram based on hierarchical cluster analysis using UPGMA algorithm was prepared. The hierarchical cluster analysis showed that the maize genotypes were divided into two main clusters. Unique maize genotype (cluster 1), Zuta Brzica, originating from Yugoslavia separated from others. Cluster 2 was divided into two main clusters (2a and 2b). Subcluster 2a contained one Yugoslavian genotype Juhoslavanska and subcluster 2b was divided in two subclusters 2ba and 2bb. The present study shows effectiveness of employing SCoT markers in analysis of maize, and would be useful for further studies in population genetics, conservation genetics and genotypes improvement.

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Evaluation of molecular diversity of central European maize cultivars

Cited by 5 publications

References 16 publications

Molecular Variability of Old Maize (Zea Mays L.) Based on Gene Specific Markers

Molecular Variability of Old Maize (Zea Mays L.) Based on Gene Specific Markers

Genetic diversity of maize resources revealed by different molecular markers

Start codon targeted (scot) polymorphism reveals genetic diversity in european old maize (Zea mays L.) Genotypes

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