Genotyping and antifungal susceptibility of human subgingival Candida albicans isolates

Pizzo, Giuseppe; Barchiesi, Francesco; Francesco, Luigi Falconi Di; Giuliana, G; Arzeni, Daniela; Milici, Maria Eleonora; D’Angelo, Matteo; Scalise, Giorgio

doi:10.1016/s0003-9969(01)00114-5

Cited by 23 publications

(29 citation statements)

References 43 publications

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“…Such genetic heterogeneity within isolates was reported from other oral and non-oral sources in C. albicans (25, 26, 56). …”

Section: Discussionsupporting

confidence: 66%

“…These methods have greatly enhanced knowledge about the epidemiology of oral and subgingival Candida species , and they can provide valuable information by their capacity to distinguish distinct isolates of the same species. Some studies have demonstrated that commensal yeasts dominate in oral candidiasis, whereas controversial evidence shows that genetically homogeneous, hypervirulent strains of C. albicans are involved in the disease (25–28). …”

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confidence: 99%

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Genetic relatedness of subgingival and buccalCandida dubliniensisisolates in immunocompetent subjects assessed by RAPD-PCR

Jewtuchowicz

Mujica

Malzone

et al. 2009

Journal of Oral Microbiology

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BackgroundIt is recognized that Candida dubliniensis commonly colonizes oral and subgingival sites in immunocompetent subjects with periodontal disease.ObjectiveSince there are few data available on genetic characterization of C. dubliniensis in periodontal pockets and other oral sites, the aim of this study was to characterize subgingival and mucosal C. dubliniensis isolates recovered from immunocompetent subjects and to assay the genetic similarity of such isolates from both niches in the same patient by random amplified polymorphic DNA (RAPD).Design C. dubliniensis recovered from subgingival plaque and from buccal cavity samples were studied in 240 immunocompetent non-smoking individuals. Arbitrary amplification was carried out by RAPD-polymerase chain reaction (PCR).ResultsRAPD analysis showed identical genotypes of C. dubliniensis in different sampling sites (buccal cavity and subgingival areas) in eight of 10 patients except for those derived from two participants who presented presumably unrelated isolates.ConclusionsOn the basis of the findings presented, the origin of the colonization of C. dubliniensis in subgingival biofilm seems to be the buccal cavity in a single patient. Consequently, it may be assumed that most of C. dubliniensis in these sites arise from the endogenous commensal strains.

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“…Such genetic heterogeneity within isolates was reported from other oral and non-oral sources in C. albicans (25, 26, 56). …”

Section: Discussionsupporting

confidence: 66%

mentioning

confidence: 99%

Genetic relatedness of subgingival and buccalCandida dubliniensisisolates in immunocompetent subjects assessed by RAPD-PCR

Jewtuchowicz

Mujica

Malzone

et al. 2009

Journal of Oral Microbiology

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“…To our knowledge, this is the first study to examine C. albicans and C. dubliniensis isolates recovered from periodontal pockets by MLST, which is now considered the gold standard for molecular typing and population analysis of Candida species. Previous studies of subgingival Candida isolates relied on less-reproducible, subjective techniques such as RAPD and electrophoretic karyotyping (1,24,33). The advantage of MLST is that it relies on species-specific DNA-sequence-based comparisons of isolates, and the data can be stored electronically in databases.…”

Section: Discussionmentioning

confidence: 99%

“…Several previous studies investigated the genetic relatedness of C. albicans isolates recovered from the periodontal pockets, gingival sulci, and oral mucosae of patients with periodontitis by using molecular typing techniques such as random amplified polymorphic DNA (RAPD) fingerprinting, electrophoretic karyotyping, and ABC genotyping, with the latter technique being based on the presence or absence of an intron in the 25S ribosomal DNA (rDNA) (1,24,32,33). Pizzo et al (24) previously used electrophoretic karyotyping to show that some C. albicans genotypes are unique to subgingival isolates, suggesting some adaptation to this environment.…”

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Enrichment of Multilocus Sequence Typing Clade 1 with Oral Candida albicans Isolates in Patients with Untreated Periodontitis

et al. 2012

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cThis study investigated the prevalence and cell density of Candida species in periodontal pockets, healthy subgingival sites, and oral rinse samples of patients with untreated periodontitis. Twenty-one periodontitis patients underwent sampling at two periodontitis sites, and 19/21 of these patients underwent sampling at one periodontally healthy site. Both paper point and curette sampling techniques were employed. The periodontitis patients and 50 healthy subjects were also sampled by oral rinse. Candida isolates were recovered on CHROMagar Candida medium, and representative isolates were identified. Candida spp. were recovered from 10/21 (46.7%) periodontitis patients and from 16/50 (32%) healthy subjects. C. albicans predominated in both groups and was recovered from all Candida-positive subjects. Candida-positive periodontitis patients yielded Candida from periodontal pockets with average densities of 3,528 and 3,910 CFU/sample from curette and paper point samples, respectively, and 1,536 CFU/ml from oral rinse samples. The majority (18/19) of the healthy sites sampled from periodontitis patients were Candida negative. The 16 Candida-positive healthy subjects yielded an average of 279 CFU/ml from oral rinse samples. C. albicans isolates were investigated by multilocus sequence typing (MLST) to determine if specific clonal groups were associated with periodontitis. MLST analysis of 31 C. albicans isolates from periodontitis patients yielded 19 sequence types (STs), 13 of which were novel. Eleven STs belonged to MLST clade 1. In contrast, 16 C. albicans isolates from separate healthy subjects belonged to 16 STs, with 4 isolates belonging to clade 1. The distributions of STs between both groups were significantly different (P ‫؍‬ 0.04) and indicated an enrichment of C. albicans isolates in periodontal pockets, which warrants a larger study.

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Fungi in Endodontic Infections

Şen¹,

Baksı²

2017

Endodontic Microbiology

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Genotyping and antifungal susceptibility of human subgingival Candida albicans isolates

Cited by 23 publications

References 43 publications

Genetic relatedness of subgingival and buccalCandida dubliniensisisolates in immunocompetent subjects assessed by RAPD-PCR

Genetic relatedness of subgingival and buccalCandida dubliniensisisolates in immunocompetent subjects assessed by RAPD-PCR

Enrichment of Multilocus Sequence Typing Clade 1 with Oral Candida albicans Isolates in Patients with Untreated Periodontitis

Fungi in Endodontic Infections

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