Genetic relatedness of subgingival and buccalCandida dubliniensisisolates in immunocompetent subjects assessed by RAPD-PCR

Abstract

BackgroundIt is recognized that Candida dubliniensis commonly colonizes oral and subgingival sites in immunocompetent subjects with periodontal disease.ObjectiveSince there are few data available on genetic characterization of C. dubliniensis in periodontal pockets and other oral sites, the aim of this study was to characterize subgingival and mucosal C. dubliniensis isolates recovered from immunocompetent subjects and to assay the genetic similarity of such isolates from both niches in the same patient by random amplified polymorphic DNA (RAPD).Design C. dubliniensis recovered from subgingival plaque and from buccal cavity samples were studied in 240 immunocompetent non-smoking individuals. Arbitrary amplification was carried out by RAPD-polymerase chain reaction (PCR).ResultsRAPD analysis showed identical genotypes of C. dubliniensis in different sampling sites (buccal cavity and subgingival areas) in eight of 10 patients except for those derived from two participants who presented presumably unrelated isolates.ConclusionsOn the basis of the findings presented, the origin of the colonization of C. dubliniensis in subgingival biofilm seems to be the buccal cavity in a single patient. Consequently, it may be assumed that most of C. dubliniensis in these sites arise from the endogenous commensal strains.

The Effect of Psychoactive Substances (Drugs) on the Presence and Frequency of Oral Candida Species and Candida Dubliniensis

“…Jewtuchowicz et al identified Candida dubliniensis in a sample of 240 patients who were alcohol and drug addicts. The incidence of Candida dubliniensis was 4.6% in the subgingival biofilm ( 18 , 19 ). Our results concur with the results of the aforementioned authors on the incidence of Candida dubliniensis in HIV-negative patients addicted to psychoactive substances.…”

Escherichia coliATCC 8739 Adapts to the Presence of Sodium Chloride, Monosodium Glutamate, and Benzoic Acid after Extended Culture

“…Primers 5, 6, and 7 amplified a random sample amounting to 0.37% of the whole genome which is a limitation of this study. However, several studies had demonstrated that PCR-based DNA fingerprinting using a small number of primers is suitable to examine genetic diversity in E. coli [60], Candida dubliniensis [61], and mackerel [62]. Another limitation is that DNA fingerprinting was performed on the entire population and not on isolated colonies.…”

Candida albicans and Candida dubliniensis in Periodontitis in Adolescents and Young Adults

“…Cuesta et al [27] reported a value of 13% of the genus Candida of which 76.2% were Candida albicans in a population of 82 with 56 chronic periodontitis, which is confirmed by several studies and which also reported that Candida albicans was the most frequent species in periodontal pockets by 21.7% to 24.4% [12,28].…”

“…According to previous studies, the distribution of Candida albicans isolates from the subgingival biofilm according to periodontal health status varied between 9,5% and 40% in subjects with chronic periodontitis, whereas in healthy subjects, the presence of Candida albicans was estimated between 3.8% and 36% [11,26,[28][29][30][31][32] and 20% in patients with aggressive periodontitis [6]. In our study, Candida albicans was recovered in the periodontal pockets of 25 (37%) subjects with periodontitis versus 17% without periodontitis (Table 2).…”

“…Few epidemiological studies have evaluated the prevalence of colonization by this species in periodontal pockets and its possible role in the oral environment of healthy and periodontitis patients [33]. A few studies have reported Candida dubliniensis colonization in the subgingival plaque of immunocompetent subjects with periodontal disease, with a prevalence of 4.4% [11] to 4.6% [28].…”