Genotypic variation in wheat grain fructan content revealed by a simplified HPLC method

Huynh, Bao‐Lam; Palmer, Lachlan; Mather, D. E.; Wallwork, H.; Graham, Robin D.; Welch, Ross M.; Stangoulis, James

doi:10.1016/j.jcs.2007.10.004

Cited by 98 publications

(100 citation statements)

References 34 publications

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“…Sugar and phytate analyses were performed using High Performance Liquid Chromatography (HPLC) on a Dionex ICS-3000 unit as described by Huynh et al [25] for fructans, Nguyen et al [24] for AXs and by Lehrfeld et al [5] for phytate. The micronutrient concentration of samples was determined by inductively coupled plasma optical emission spectrometry (ICP-OES) [26] using an ARL 35B ICP analyser.…”

Section: Methodsmentioning

confidence: 99%

The effect of wheat prebiotics on the gut bacterial population and iron status of iron deficient broiler chickens

Tako

Glahn

Knez

et al. 2014

Nutr J

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BackgroundCurrently, there is a lot of interest in improving gut health, and consequently increasing Fe absorption, by managing the colonic microbial population. This is traditionally done by the consumption of probiotics, live microbial food supplements. However, an alternative, and often very effective approach, is the consumption of food ingredients known as prebiotics. Fructans and arabinoxylans are naturally occurring non-digestible oligosaccharides in wheat that exhibit prebiotic properties and may enhance intestinal iron (Fe) absorption. The aim of this study was to assess the effect of prebiotics from wheat on Fe bioavailability in vitro (Caco-2 cells) and in vivo (broiler chickens, Gallus gallus).MethodsIn the current study, the effect of intra-amniotic administration of wheat samples extracts at 17 d of embryonic incubation on the Fe status and possible changes in the bacterial population in intestinal content of broiler hatchlings were investigated. A group of 144 eggs were injected with the specified solution (1 ml per egg) into the amniotic fluid. Immediately after hatch (21 d) and from each treatment group, 10 chicks were euthanized and their small intestine, liver and cecum were removed for relative mRNA abundance of intestinal Fe related transporters, relative liver ferritin amounts and bacterial analysis of cecal content, respectively.ResultsThe in vivo results are in agreement with the in vitro observations, showing no differences in the hatchling Fe status between the treatment groups, as Fe bioavailability was not increased in vitro and no significant differences were measured in the intestinal expression of DMT1, Ferroportin and DcytB in vivo. However, there was significant variation in relative amounts of bifidobacteria and lactobacilli in the intestinal content between the treatments groups, with generally more bifidobacteria being produced with increased prebiotic content.ConclusionsIn this study we showed that prebiotics naturally found in wheat grains/bread products significantly increased intestinal beneficial bacterial population in Fe deficient broiler chickens. With this short-term feeding trial we were not able to show differences in the Fe-status of broilers. Nevertheless, the increase in relative amounts of bifidobacteria and lactobacilli in the presence of wheat prebiotics is an important finding as these bacterial populations may affect Fe bioavailability in long-term studies.

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Section: Methodsmentioning

confidence: 99%

The effect of wheat prebiotics on the gut bacterial population and iron status of iron deficient broiler chickens

Tako

Glahn

Knez

et al. 2014

Nutr J

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“…Grain fructan concentration was measured by enzymatic hydrolysis followed by high-performance liquid chromatography (HPLC) using procedures described by Huynh et al (2008). After extraction of wheat flour, the hydrolysates (glucose, fructose and sucrose) were separated as anions with KOH eluent through a CarboPac PA20 guard column (3 Â 30 mm) coupled to a CarboPac PA20 analytical column (3 Â 150 mm) and detected by pulsed amperometry on a disposable gold working electrode.…”

Section: Grain Fructanmentioning

confidence: 99%

Wheat grain quality under increasing atmospheric CO2 concentrations in a semi-arid cropping system

Fernando

Panozzo²,

Tausz

et al. 2012

Journal of Cereal Science

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“…They also stimulate blood flow to the colon and the autonomic nervous system [16]. Lentils have 13gr/100gr probiotic carbohydrates [17]. Fructooligosaccarids and galactooligosaccharids are well-known for their prebiotic action [18].…”

Section: Carbohydratementioning

confidence: 99%

Lentil’s (Lens Culinaris L.) Functional Properties in Prevention and Treatment of Non-Communicable Chronic Diseases: A Review

Aslani¹,

Alipour

Mirmiran³

et al. 2015

IJNFS

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Abstract:Background: using functional food in diet planning and diet therapy is one of the newest approaches in prevention and treatment of non-communicable chronic diseases. Lentil has many bioactive and functional compounds and we have reviewed the influence of lentil in prevention and treatment of chronic diseases in this review study. Materials and methods: In order to do search about mentioned objective key words including Lentils (Lens culinarisL.), functional foods, bioactive peptides, nutritional value, health polyphenol in combination with glycemic index (GI), insulin resistance, diabetes, cancer and hypertension, the sources in PubMed database were examined in the years between1986-2013. Key words such as lentils (Lens culinarisL.), functional foods, bioactive peptides, nutritional value, diabetes, cancer and hypertension were studied with access to Persian sources of Scientific Information Database (SID). Cell studies, animal models, clinical studies and review articles were used with favorable quality. Results: Lentils are rich sources of fibers, resistant starches, prebiotic compounds, phytochemicals, proteins and bioactive peptides, phenolic acids and antioxidants. Lentil has the highest total antioxidant capacity among other foods including apples, dates, raspberries, cherries, figs, oranges, garlic, cabbage and peanuts. High content of fibers and other phytochemicals are found in lentils that could improve glycemic response in diabetic patients, lipid and lipoprotein metabolism and weight management. Lentils may have favorable effects in prevention of diabetes, cancer and cardiovascular diseases (CVD s) prevalence. Conclusion: Increased dietary intake of lentils is considered as an important key in prevention and treatment of chronic diseases, especially type 2 diabetes, cardiovascular and cancer diseases.

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Genotypic variation in wheat grain fructan content revealed by a simplified HPLC method

Cited by 98 publications

References 34 publications

The effect of wheat prebiotics on the gut bacterial population and iron status of iron deficient broiler chickens

The effect of wheat prebiotics on the gut bacterial population and iron status of iron deficient broiler chickens

Wheat grain quality under increasing atmospheric CO2 concentrations in a semi-arid cropping system

Lentil’s (Lens Culinaris L.) Functional Properties in Prevention and Treatment of Non-Communicable Chronic Diseases: A Review

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