Genotypic variation among wild isolates of two nuclear polyhedrosis viruses isolated from Spodoptera littoralis

Cherry, Carol L.; Summers, Max D.

doi:10.1016/0022-2011(85)90071-0

Cited by 62 publications

(35 citation statements)

References 18 publications

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“…Comparisons of restriction endonuclease fragment pro files is an important tool to identify molecular differences among various viral species and strains ( Smith & Sum mers, 1978;McCarthy et al, 1979;;Maruniak et al, 1984;Cherry & Summer, 1985;Shapiro & Dougherty, 1985;Kuzio et al, 1999). The viral genome DNA of LdMNPVF, a624, and LdMNPVK digested with BamHI, EcoRI, HindlII, and Notl showed similarities (Fig.…”

Section: Resultsmentioning

confidence: 99%

A gypsy moth (Lymantria dispar, Lepidoptera: Lymantriidae) multinucleocapsid nuclear polyhedrosis virus from France: comparison with a North American and a Korean strain

Narang¹,

Hérard²,

Dougherty³

et al. 2001

Eur. J. Entomol.

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Section: Resultsmentioning

confidence: 99%

A gypsy moth (Lymantria dispar, Lepidoptera: Lymantriidae) multinucleocapsid nuclear polyhedrosis virus from France: comparison with a North American and a Korean strain

Narang¹,

Hérard²,

Dougherty³

et al. 2001

Eur. J. Entomol.

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“…Especially, SpliNPV-B was reported as a suitable candidate for use as a microbial pest control agent . SpliNPV-B variants have been isolated from diseased S. littoralis larvae collected in Israel, Egypt and Morocco and from diseased Spodoptera litura Fabricius larvae collected in Japan to date (Cherry and Summers 1985;Maeda et al 1990;Kislev and Edelman 1982). The newest SpliNPV-B variant was recently isolated from field-infected S. littoralis larvae in Mersin, Turkey, and defined as the first Turkish isolate (TR1) and the fifth isolate of SpliNPV-B worldwide (Toprak and Gürkan 2004).…”

Section: Introductionmentioning

confidence: 99%

Norduz Erkek Kuzularında Testis Özellikleri ve Serum Testosteron Konsantrasyonun Yaşa Göre Değişimi1

Ayhan;CENGİZ¹

2006

Tarım Bilimleri Dergisi

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Spodoptera littoralis Boisd. (Lepidoptera:Noctuidae) nucleopolyhedrovirus (SpliNPV) is a member of Baculoviridae and belongs to the group II NPVs. Lef-8 is a well-conserved gene that is characterized in most of the lepidopteran NPVs. Nucleotide and aminoacid sequences of the encoded proteins and gene content may be phylogenetically informative. In this study, lef-8 gene in the Turkish SpliNPV-B (SpliNPV-TR1) genome was partially amplified by PCR and analyzed by amino-acid sequences with several baculoviruses. Amino-acid sequences of partial SpliNPV-TR1 lef-8 gene was 84 % identical to the lef-8 of SpltNPV while revealed it to have about only 57 %, 55 %, 51 %, 50 %, 50 %, 50 % and 49 % of its amino-acid sequence identity with AgipNPV, McNPV, RoNPV, HaNPV-G4, AcNPV, HzNPV and BufuNPV, respectively. The sequences between SpliNPV-TR1 and granuloviruses also produced low levels of sequence identities (maximum 46 %). Although group II NPVs were rather isolated from noctuid hosts, the low level identity values detected between these noctuid NPVs (except the high idendity value between SpliNPV-TR1 and SpltNPV) revealed that these isolates can not have a monophyletic origin.Key Words: Baculovirus, spodoptera littoralis, noctuidae, spliNPV-TR1, lef-8, sequence, comparison. Türk SpliNPV-B İzolatından elde edilen lef-8 Geninin diğer Bakulovirüslerle Kısmi Sekans KarşılaştırmasıÖz: Spodoptera littoralis Boisd. (Lepidoptera:Noctuidae) nukleopolihedrovirus (SpliNPV) Baculoviridae'nin bir üyesi olup grup II NPV'leri arasında yer almaktadır. Lef-8 iyi korunmuş bir gen olup pek çok lepidopter NPV'sinde tanımlanmıştır. Kodlanan proteinlerin nükleotid ve aminoasit dizinleri ve gen içerikleri filogenetik olarak aydınlatıcı olabilmektedir. Bu çalışmada, Türk SpliNPV-B (SpliNPV-TR1) genomundaki lef-8 geni PCR'la kısmi olarak çoğaltılmış ve aminoasit dizinlerine göre bazı bakulovirüslerle analiz edilmiştir. SpliNPV-TR1 kısmi lef-8 geninin aminoasit dizinleri SpltNPV ile % 84 benzelik gösterirken AgipNPV, McNPV, RoNPV, HaNPV-G4, AcNPV, HzNPV ve BufuNPV ile sırasıyla sadece % 57, % 55 , % 51, % 50, % 50, % 50 ve % 49 aminoasit benzerliği göstermiştir. SpliNPV-TR1 ve granulovirüsler arasındaki sekanslar da düşük dizin benzerlikleri üretmiştir (en fazla % 46). Grup II NPV'leri esas olarak noctuid konukçulardan izole edilse de, bu noctuid NPV'leri arasında saptanan düşük dereceli benzerlikler (SpliNPV-TR1 ve SpltNPV arasındaki yüksek benzerlik oranı hariç), bu izolatların monofiletik bir orijine sahip olamayacağını göstermektedir.

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“…NPVs cause lethal epizootic diseases in their host-insect populations and because of their widespread occurence among economically important insect pests, they have received considerable attention as microbial pesticides (Payne 1988). Different SpliNPV isolates have also been isolated from egyptian cotton worm, S. littoralis to date and these isolates were designated SpliMNPV-A, SpliMNPV-B (Cherry and Summers 1985) and SpliMNPV-C (Maeda et al 1990) according to their restriction-fragment profiles. The ability of SpliMNPV-B to successfully infect several Spodoptera species including Spodoptera exigua Hübner (Merdan et al 1977), Spodoptera exempta Walker, Spodoptera frugiperda Smith (McKinley et al 1981) and Spodoptera litura Fabricius (Okada 1977), make it a suitable candidate for use as a microbial pest control agent (Faktor et al 1997).…”

Section: Introductionmentioning

confidence: 99%

“…The ability of SpliMNPV-B to successfully infect several Spodoptera species including Spodoptera exigua Hübner (Merdan et al 1977), Spodoptera exempta Walker, Spodoptera frugiperda Smith (McKinley et al 1981) and Spodoptera litura Fabricius (Okada 1977), make it a suitable candidate for use as a microbial pest control agent (Faktor et al 1997). SpliNPV-B variants have been isolated from diseased S. littoralis larvae collected in Israel, Egypt and Morocco and Japan (Cherry and Summers 1985, Kislev and Edelman, 1982, Maeda et al 1990). The fifth sample of a SpliNPV-B variant was isolated from diseased S. littoralis larvae in 2002 in Turkey.…”

Section: Introductionmentioning

confidence: 99%

Gross Pathology of SpliNPVs and Alterations in Spodoptera littoralis Boisd. (Lepidoptera:Noctuidae) Morphology Due to Baculoviral Infection*

;BAYRAM¹

2005

Tarım Bilimleri Dergisi

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Baculoviruses are invertebrate-specific pathogens and baculoviral infections cause alterations in the physiology, metabolism and morphology of insects. It is important to recognize these physiological and symptomatologic changes to understand baculovirus infection cycle and biology. For this reason, in our study Spodoptera littoralis Boisd. (Lepidoptera: Noctuidae) larvae were inoculated with the SpliNPV to doses of 3000 and 20,000 OBs for third instars and to concentrations of 10 6 and 3x 10 6 OBs/ml for neonates and the alterations due to NPV infection in larvae were then examined. Bioassays carried out with the third instar S. littoralis larvae revealed that no symptoms were detected during the first three days post-inoculation due to SpliNPV infection. Approximately, in the 4th day, infected larvae began to respond much more slowly than healthy larvae. This symptom was followed by whitening and slimming of cuticula, failure in molting, swelling of the body, diarrhoea, climbing to high places and hanging, liquefaction of the body and death. Infected neonates exhibited limited symptoms of distension and the failure of molting. The larvae died in approximately 8-8.5 days for third instars and in approximately 3-3.5 days for neonates due to baculoviral infection for the both doses and concentrations. On the other hand, different doses or concentrations did not cause an alteration in the occurence time of symptoms. To associate the NPV biology with the genes involved in baculovirus genome and understand their life cycle will improve their efficacy as biopesticides and help to the effective use of baculoviruses. 6 and 3x 10 6 OBs/ml konsantrasyonlarında SpliNPV ile inokule edilmiş ve daha sonra larvalarda NPV enfeksiyonundan kaynaklanan değişimler irdelenmiştir. Üçüncü dönem S. littoralis larvalarıyla yapılan denemelerde inokulasyonu takip eden ilk üç gün boyunca SpliNPV enfeksiyonundan kaynaklı simptom görülmediği saptanmıştır. Yaklaşık olarak dördüncü günle birlikte hastalıklı larvalar sağlıklı larvalara göre daha yavaş tepkiler vermiştir. Bu simptomu, kutikulanın beyazlaşması ve incelmesi, deri değiştirememe, vücudun şişmesi, ishal durumu, yüksek yerlere tırmanma ve asma, vücudun sıvılaşması ve ölüm takip etmiştir. Enfekteli birinci dönem larvalar ise şişme ve deri değiştirememeyi içeren kısıtlı simptomlar vermiştir. Bakulovirüs enfeksiyonundan dolayı 3. dönem larvalar 8-8.5 günde, 1. dönem larvalar ise 3-3.5 günde her iki doz ve konsantrasyonda ölmüştür. Diğer taraftan farklı doz ya da konsantrasyonlar, simptomların ortaya çıkış zamanında bir değişikliğe neden olmamıştır. NPV bioyolojisini bakulovirus genomunda yer alan genlerle ilişkilendirmek ve hayat döngülerini anlamak bunların birer biyopestisit olarak etkinliğini artıracak ve daha etkin kullanımlarına yardımcı olacaktır.

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Genotypic variation among wild isolates of two nuclear polyhedrosis viruses isolated from Spodoptera littoralis

Cited by 62 publications

References 18 publications

A gypsy moth (Lymantria dispar, Lepidoptera: Lymantriidae) multinucleocapsid nuclear polyhedrosis virus from France: comparison with a North American and a Korean strain

A gypsy moth (Lymantria dispar, Lepidoptera: Lymantriidae) multinucleocapsid nuclear polyhedrosis virus from France: comparison with a North American and a Korean strain

Norduz Erkek Kuzularında Testis Özellikleri ve Serum Testosteron Konsantrasyonun Yaşa Göre Değişimi1

Gross Pathology of SpliNPVs and Alterations in Spodoptera littoralis Boisd. (Lepidoptera:Noctuidae) Morphology Due to Baculoviral Infection*

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