A gypsy moth (Lymantria dispar, Lepidoptera: Lymantriidae) multinucleocapsid nuclear polyhedrosis virus from France: comparison with a North American and a Korean strain

Narang, Neelam; Hérard, Franck; Dougherty, Edward M.; Chen, Kim; Vega, Fernando E.

doi:10.14411/eje.2001.035

Cited by 13 publications

(8 citation statements)

References 26 publications

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“…There are also two subspecies, L. dispar asiatica and L. dispar japonica, which are part of the Asian gypsy moth complex (Pogue and Schaefer, 2007) The isolate Lymantria dispar multiple nucleopolyhedrovirus LDP-67 (LdMNPV LDP-67) has formed the basis for Gypchek, a biocontrol product currently produced by the USDA Forest Service and Sylvar Technologies Inc. (Canada) for use against outbreaks of gypsy moth in North America (Reardon et al, 2012). Other LdMNPV isolates have been tested for activity against gypsy moth populations in North America and Asia (Duan et al, 2012;Lewis et al, 1984;Narang et al, 2001;Shapiro et al, 1984). An isolate has also been used to develop the product Virin-ENSh for use against gypsy moth in the former Soviet Union (Alyoshina, 1980).…”

Section: Introductionmentioning

confidence: 99%

Geographic isolates of Lymantria dispar multiple nucleopolyhedrovirus: Genome sequence analysis and pathogenicity against European and Asian gypsy moth strains

Harrison

Rowley

Keena

2016

Journal of Invertebrate Pathology

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Isolates of the baculovirus species Lymantria dispar multiple nucleopolyhedrovirus have been formulated and applied to suppress outbreaks of the gypsy moth, L. dispar. To evaluate the genetic diversity in this species at the genomic level, the genomes of three isolates from Massachusetts, USA (LdMNPV-Ab-a624), Spain (LdMNPV-3054), and Japan (LdMNPV-3041) were sequenced and compared with four previously determined LdMNPV genome sequences. The LdMNPV genome sequences were collinear and contained the same homologous repeats (hrs) and clusters of baculovirus repeat orf (bro) gene family members in the same relative positions in their genomes, although sequence identities in these regions were low. Of 146 non-bro ORFs annotated in the genome of the representative isolate LdMNPV 5-6, 135 ORFs were found in every other LdMNPV genome, including the 37 core genes of Baculoviridae and other genes conserved in genus Alphabaculovirus. Phylogenetic inference with an alignment of the core gene nucleotide sequences grouped isolates 3041 (Japan) and 2161 (Korea) separately from a cluster containing isolates from Europe, North America, and Russia. To examine phenotypic diversity, bioassays were carried out with a selection of isolates against neonate larvae from three European gypsy moth (Lymantria dispar dispar) and three Asian gypsy moth (Lymantria dispar asiatica and Lymantria dispar japonica) colonies. LdMNPV isolates 2161 (Korea), 3029 (Russia), and 3041 (Japan) exhibited a greater degree of pathogenicity against all L. dispar strains than LdMNPV from a sample of Gypchek. This study provides additional information on the genetic diversity of LdMNPV isolates and their activity against the Asian gypsy moth, a potential invasive pest of North American trees and forests.

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Section: Introductionmentioning

confidence: 99%

Geographic isolates of Lymantria dispar multiple nucleopolyhedrovirus: Genome sequence analysis and pathogenicity against European and Asian gypsy moth strains

Harrison

Rowley

Keena

2016

Journal of Invertebrate Pathology

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“…The Lymantria dispar multinucleopolyhedrovirus (LdMNPV, Baculoviridae) is a specialized virus inducing natural epizootics in gypsy moth populations and is widely used to control L . dispar in many countries [ 28 – 29 ]. To substantiate the potential resistance of larvae against the virus, we estimated the level of its actual resistance to the baculovirus.…”

Section: Introductionmentioning

confidence: 99%

Asynchrony between Host Plant and Insects-Defoliator within a Tritrophic System: The Role of Herbivore Innate Immunity

et al. 2015

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The effects of asynchrony in the phenology of spring-feeding insect-defoliators and their host plants on insects’ fitness, as well as the importance of this effect for the population dynamics of outbreaking species of insects, is a widespread and well-documented phenomenon. However, the spreading of this phenomenon through the food chain, and especially those mechanisms operating this spreading, are still unclear. In this paper, we study the effect of seasonally declined leafquality (estimated in terms of phenolics and nitrogen content) on herbivore fitness, immune parameters and resistance against pathogen by using the silver birch Betula pendula—gypsy moth Lymantria dispar—nucleopolyhedrovirus as the tritrophic system. We show that a phenological mismatch induced by the delay in the emergence of gypsy moth larvae and following feeding on mature leaves has negative effects on the female pupal weight, on the rate of larval development and on the activity of phenoloxidase in the plasma of haemolymph. In addition, the larval susceptibility to exogenous nucleopolyhydrovirus infection as well as covert virus activation were both enhanced due to the phenological mismatch. The observed effects of phenological mismatch on insect-baculovirus interaction may partially explain the strong and fast fluctuations in the population dynamics of the gypsy moth that is often observed in the studied part of the defoliator area. This study also reveals some indirect mechanisms of effect related to host plant quality, which operate through the insect innate immune status and affect resistance to both exogenous and endogenous virus.

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“…On the other hand, in the Murillo et al (2) study; some strains of nucleopolyhedrovirus obtained from different geographical areas showed different insecticidal activities. For instance, the LdMNPV strain from France was the least active, whereas the North American strain had the highest activity (1). Additionally, the Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) is a relatively virulent species.…”

Section: Discussionmentioning

confidence: 99%

“…The gypsy moth, Lymantria dispar L. (Lepidoptera, Lymantriidae) is a common pest of forests and fruit trees throughout the world (1). This insect is also a major serious pest in Turkey.…”

Section: Introductionmentioning

confidence: 99%

The First Record of Nucleopolyhedrovirus Isolated from the Gypsy Moth, Lymantria dispar (Lepidoptera, Lymantriidae) in Turkey

Yaman¹,

Bekircan²,

Radek³

et al. 2012

Turkiye Parazitol Derg

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Objective: The gypsy moth, Lymantria dispar L. (Lepidoptera, Lymantriidae) is a common pest of forests and fruit trees throughout the world. This insect is also a major serious pest in Turkey. Nowadays L. dispar can be managed by biological control methods especially, using entomopathogenic viruses. The aim of this study is to characterize entomopathogenic viruses and is the first record of nucleopolyhedrovirus isolated from the gypsy moth in Turkey. Methods: PIBs obtained from infected larvae were measured and photographed using an Olympus BX51 microscope with a DP-25 digital camera and a DP2-BSW Soft Imaging System and examined with a Philips 208 electron microscope (TEM). Results: The virus had the typical characteristics of nucleopolyhedroviruses. The dimension of the polyhedral inclusion bodies (PIBs) was 2.03±0.25 µm. PIBs varied in size from 1.65 to 2.21 µm and were usually polygonal in shape. Virions in PIBs contained 1 to 8 nucleocapsids per virion. The size of the viral particles was 366.67±54.72 (312-500) x 42.95±6.12 (30-47) nm. Conclusion: The isolation and characterization of a pure isolate of Lymantria dispar multinucleopolyhedrovirus (LdMNPV-TR) from Turkey is presented for the first time. (Turkiye Parazitol Derg 2012; 36: 92-5)

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A gypsy moth (Lymantria dispar, Lepidoptera: Lymantriidae) multinucleocapsid nuclear polyhedrosis virus from France: comparison with a North American and a Korean strain

Abstract: Abstract. As part of a search for natural enemies of the gypsy moth (Lymantria dispar), virus-infected samples were collected near Toulouse, France. Light and electron microscope studies confirmed that the French strain is a multinucleocapsid nuclear polyhe drosis virus (MNPV).

Cited by 13 publications

References 26 publications

Geographic isolates of Lymantria dispar multiple nucleopolyhedrovirus: Genome sequence analysis and pathogenicity against European and Asian gypsy moth strains

Geographic isolates of Lymantria dispar multiple nucleopolyhedrovirus: Genome sequence analysis and pathogenicity against European and Asian gypsy moth strains

Asynchrony between Host Plant and Insects-Defoliator within a Tritrophic System: The Role of Herbivore Innate Immunity

The First Record of Nucleopolyhedrovirus Isolated from the Gypsy Moth, Lymantria dispar (Lepidoptera, Lymantriidae) in Turkey

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