Genotypic variation among Staphylococcus aureus isolates from cases of clinical mastitis in Swedish dairy cows

Capurro, A.; Aspán, Anna; Artursson, Karin; Waller, Karin Persson

doi:10.1016/j.tvjl.2009.05.007

Cited by 17 publications

(24 citation statements)

References 26 publications

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“…On the other hand, other PFGE clusters (except for cluster IX) were typically found only on one of the farms. Clonal spread of S. aureus mastitis isolates has been previously described at both the regional and farm level (Capurro et al 2010;Fessler et al 2010;Castelani et al 2013). Such clonal spread indicates that certain lineages of S. aureus mastitis strains have proven to be particularly successful.…”

Section: Discussionmentioning

confidence: 90%

Prevalence of mastitis pathogens in milk from clinically healthy cows

Cervinkova¹,

Vlkova²,

Borodacova³

et al. 2013

Vet. Med.

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A total of 669 individual cow milk samples originating from asymptomatic cows from 16 dairy farms were examined for the presence of microorganisms with the potential to cause mastitis. Coagulase-negative staphylococci clearly predominated (53.5% positive samples) followed by streptococci and enterococci (both occurring in 16.1% samples). Among streptococci, so-called mastitis streptococci (S. uberis, S. dysgalactiae and S. agalactiae) prevailed (11.7% positive samples). Enterobacteriaceae were found in 10.0% samples, most of which (6.6% samples) were positive for Escherichia coli. Yeasts (mainly Candida spp.) were found in 8.2% samples. One of the major mastitis pathogens, Staphylococcus aureus subsp. aureus, was isolated from 9.0% of samples. S. aureus isolates were further characterised in terms of their capability to form biofilm, antimicrobial susceptibility and clonality (PFGE). All S. aureus isolates were capable of biofilm formation and were generally susceptible to the majority of tested antibiotics. The exception was ampicillin, resistance to which was observed in 27.7% isolates. Therefore, the relatively frequent occurrence of S. aureus could be attributed to persistent intramammary infections due to biofilm formation rather than low efficacy of particular antibiotics. PFGE analysis revealed clonal spread of certain S. aureus isolates within and between farms indicating that certain lineages of S. aureus mastitis strains are particularly successful.

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Section: Discussionmentioning

confidence: 90%

Prevalence of mastitis pathogens in milk from clinically healthy cows

Cervinkova¹,

Vlkova²,

Borodacova³

et al. 2013

Vet. Med.

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“…aureus mastitis. Some CC occur worldwide (Fitzgerald et al, 1997;Smith et al, 2005b), whereas others may have a local or regional distribution (Guidry et al, 1998;Capurro et al, 2010). Clonal complex CC97 was frequently identified among isolates from Brazil (Aires- de-Sousa et al, 2007;Rabello et al, 2007), and the United Kingdom, United States, and Chile (Smith et al, 2005b), but was uncommon in bulk milk samples from Norway (Jørgensen et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Host adaptation of bovine Staphylococcus aureus seems associated with bacteriological cure after lactational antimicrobial treatment

Borne

Nielen

Schaik

et al. 2010

Journal of Dairy Science

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Staphylococcus aureus causes a wide range of diseases in multiple species. Some sequence types (ST) are observed in a variety of hosts, whereas other strains are mainly associated with bovine mastitis, suggesting host adaptation. We propose that host adaptation of Staph. aureus may influence bacteriological cure of bovine subclinical mastitis after antimicrobial treatment. To test this hypothesis, multilocus sequence typing was performed on Staph. aureus isolates from 60 treated and 79 untreated control quarters that were obtained from well-defined cohorts of dairy cows from a recently conducted randomized field trial on early treatment of subclinical mastitis. Bovine-associated ST were distinguished from non-bovine-associated ST based on the literature and public databases. The association between host adaptation and bacteriological cure was investigated using population-averaged logistic regression models. Thirteen ST were identified, with approximately 80% of isolates belonging to bovine-associated ST. The odds for cure were around 2.5 times as high for non-bovine-associated ST as for bovine ST in treated quarters, whereas no difference in spontaneous cure was observed in untreated control quarters. In addition, host adaptation was related to known predictors of cure, such as penicillin susceptibility and somatic cell count. All isolates belonging to non-bovine-associated ST were resistant to penicillin, whereas the majority of isolates belonging to bovine-associated ST were penicillin susceptible. Penicillin-resistant bovine-associated strains were associated with high somatic cell counts compared with other strains. The correlation between penicillin resistance, cell counts, and host adaptation may affect the association between host adaptation and cure. For diagnostic purposes, a simple and fast alternative to multilocus sequence typing of Staph. aureus to determine host adaptation may be valuable.

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“…Staphylococcus aureus is regarded as enterotoxins, leukocidins and coagulase (Hata et al 2006, Peles et al 2007, Capurro et al 2010. α-haemolysin has haemolytic, dermonecrotic and neurotoxic activity and is considered main pathogenicity factor of S. aureus (Dinges et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Virulence factors and ability of staphylococci from bovine milk and the cowshed environment to biofilm formation

Kot¹,

Binek²,

Piechota³

et al. 2013

Polish Journal of Veterinary Sciences

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The aim of this study was to examine virulence factors and the ability of S. aureus and CNS species isolated from milk of cows with mastitis to form biofilm, and to compare them with virulence factors of staphylococci from milk of cows without mastitis and cowshed environment. Most of S. aureus strains from cows with mastitis showed haemolytic activity (93.9%), among them 72.7% and 21.2% produced α-and β-haemolysin, respectively. S. aureus from cows with mastitis symptoms produced proteases (above 48%) and esterase (42.4%). The highly significant relationship between the number of S. xylosus strains producing haemolysins (62%) and the origin of these strains from milk of cows with mastitis was observed. The ability to produce proteases was significantly associated with S. sciuri from milk of cows with mastitis. The ability of biofilm formation by staphylococcal strains from milk of cows with mastitis was greater than in strains from milk of cows without mastitis and the difference was significant (p≤0.05). The highest percentage of strains from milk of cows with mastitis were weak biofilm formers (48.6%), while 40% and 11.4% of strains were moderate and strong biofilm producers, respectively. S. xylosus showed the highest ability to form biofilm, while the lowest ability to form biofilm was observed in S. aureus and S. epidermidis.In conclusion, production of exotoxins and enzymes, and ability of biofilm formation shown by many CNS isolated from milk of cows with mastitis symptoms indicates that these features are important in pathogenesis of this disease.

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Genotypic variation among Staphylococcus aureus isolates from cases of clinical mastitis in Swedish dairy cows

Cited by 17 publications

References 26 publications

Prevalence of mastitis pathogens in milk from clinically healthy cows

Prevalence of mastitis pathogens in milk from clinically healthy cows

Host adaptation of bovine Staphylococcus aureus seems associated with bacteriological cure after lactational antimicrobial treatment

Virulence factors and ability of staphylococci from bovine milk and the cowshed environment to biofilm formation

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