Genotypic diversity, antimicrobial resistance and biofilm-forming abilities of Campylobacter isolated from chicken in Central China

Abstract

Background Campylobacter is considered to be the leading cause of human bacterial gastroenteritis, of which poultry is the main reservoir. Campylobacter contaminated chicken products are a major cause of human Campylobacter infection. In this study, the prevalence of Campylobacter in chicken in central China was investigated, and the genotypic diversity, antimicrobial resistance and biofilm of these isolates were characterized.ResultsA total of 206 Campylobacter isolates, including 166 C. jejuni and 40 C. coli, were isolated from chicken farms and live poultry markets in central China. Multilocus sequence typing and phylogenetic analysis showed that the Campylobacter isolates had diverse genetic backgrounds, which covered most of the dominant clone complexes (CCs) reported throughout China. The most prevalent CCs were CC-464, CC-1150, CC-353, and CC-828. All the isolates showed resistance to norfloxacin, ciprofloxacin and Cefazolin, and a prevalent resistance to fluoroquinolones, β-lactams and tetracyclines was also observed. Among all the isolates, 133 strains showed the ability to form biofilm, thereinto, the isolates in two genetic branches, mainly including CC-21, CC-48, CC-677 and CC-45, showed a significantly lower ability to form biofilm than other genetic branches (p < 0.05). However, in general, the ability to form biofilm varied among different genetic branches, suggesting a complex genetic background to biofilm formation, but not only the genetic lineages. Compared with the strains unable to form biofilm, biofilm-producing strains possessed a significantly higher resistance to ampicillin, neomycin, sulfamethoxazole, amikacin, clindamycin and erythromycin (p < 0.05).ConclusionsTo the best of our knowledge, this is the first report on the relationship of the genotypic diversity, antimicrobial resistance and biofilm-forming abilities of Campylobacter isolated from chicken in Central China, which showed the potential importance of biofilm in antimicrobial resistance. This study will help us better understand the epidemiology and antimicrobial resistance of Campylobacter.Electronic supplementary materialThe online version of this article (10.1186/s13099-017-0209-6) contains supplementary material, which is available to authorized users.

Molecular Characterization and Antibiotic Resistant Profiles of Campylobacter Species Isolated From Poultry and Diarrheal Patients in Southeastern China 2017–2019

“…The dominant CCs in this study were CC-21 and CC-45 and were in agreement with prior findings from other countries in which CC-21 and CC-45 appear to be the largest complexes (Sheppard et al, 2010;Guyard-Nicodème et al, 2015;An et al, 2018). The prevalence of CC-353, CC-464, and CC-828 was lower than those in other regions of China (Zhang et al, 2017), supporting the idea that Campylobacter subtype diversity may differ by region and sampled area. The association between CCs and hosts was previously noted and was reaffirmed by the present survey.…”

“…To quantify Campylobacter diversity and aid in controlling Campylobacter-borne infection, we characterized the distribution, subtype diversity, and antimicrobial susceptibility of 195 Campylobacter isolates collected from representative poultry products and humans in Southeastern China. A total of 55.8% of representative poultry samples were contaminated with Campylobacter, which was higher than that detected in other regions of China (Huang et al, 2009;Ma et al, 2017;Zhang et al, 2017). Chickens (64%) were identified as the major source of infection in the study area, while the contamination rate of Campylobacter spp.…”

Molecular Identification of Multidrug-Resistant Campylobacter Species From Diarrheal Patients and Poultry Meat in Shanghai, China

“…Antibiotic usage in both animal agriculture and human medicine can influence the development of antibiotic resistance ( Luangtongkum et al, 2009 ). Unfortunately, multidrug resistant Campylobacter isolates are now creeping from food supply chain ( Ma et al, 2014 , 2017 ; Zhang et al, 2014 , 2016 ; Wang et al, 2016 ; Wei et al, 2016 ; Zhong et al, 2016 ) into our community ( Pan et al, 2015 ; Zhang et al, 2017 ). Multidrug resistant Campylobacter isolates are big risks to public health.…”

Point Deletion or Insertion in CmeR-Box, A2075G Substitution in 23S rRNA, and Presence of erm(B) Are Key Factors of Erythromycin Resistance in Campylobacter jejuni and Campylobacter coli Isolated From Central China

“…Contaminated poultry products are recognized as the main source of human infection (Zhang T. et al, 2016). In view of the high incidence of fluoroquinolone resistance, including in China (Zhang et al, 2017b), macrolides, such as erythromycin, are the first-line choice for treatment of campylobacteriosis (Bolinger and Kathariou, 2017). Although erythromycin has been limited for use in animal production in China in 2000, the incidence of erythromycin resistance in Campylobacter continues to increase Du et al, 2018).…”